Abstract: The present invention relates to a method for determining a slight amount of NADH or XTP which is present in a solution, with high sensitivity by the use of an NADH kinase specific for NADH by utilizing a cycling reaction, and this method permits highly sensitive determination of NADH without any influence of NAD.sup.+ and hence is useful for diagnoses of diseases and the like.
Type:
Grant
Filed:
January 21, 1992
Date of Patent:
October 5, 1993
Assignees:
Noda Institute for Scientific Rsearch, International Reagents Corp.
Inventors:
Tsuyoshi Ohno, Masaru Suzuki, Tatsuo Horiuchi, Yasushi Shirahase, Koji Kishi, Yoshifumi Watazu