Abstract: The present invention provides an approach for the determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, expression markers and other criteria, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of modulators of cellular activation allows for characterization of pathways and cell populations.
Type:
Application
Filed:
August 3, 2012
Publication date:
February 7, 2013
Applicant:
Nodality, Inc. A Delaware Corporation
Inventors:
David B. Rosen, Santosh K. Putta, Brent Louie, Norman Purvis
Abstract: An embodiment of the present invention is a method for measuring the post translational states and expression levels of proteins in the PI3K and/or mTor for use in diagnosis, prognosis and drug screening applications.
Abstract: An embodiment of the present invention is a method for subjecting a hematopoetic cell to a JAK/STAT inhibitor, determining the activity of gain-of-function mutations of a Jak family kinase, determining the expression levels and activity of JAK/STAT regulatory proteins, correlating the expression levels and the activity of JAK/STAT regulatory proteins with the activity of gain-of-function mutations of a Jak family kinase and with a response to the JAK/STAT inhibitor, and then classifying the cells. A further embodiment of the invention includes determining the clinical outcome based on the cell classification, determining a method of treatment, determining dosing and scheduling of at least one of the JAK/STAT inhibitors or other compounds.
Type:
Application
Filed:
January 14, 2010
Publication date:
August 19, 2010
Applicant:
Nodality, Inc., A Delaware Corporation
Inventors:
Wendy J. Fantl, Alessandra Casano, Todd Covey
Abstract: The invention provides systems, compositions, kits and methods for automated processing of biological samples and analysis using a flow cytometer.
Type:
Application
Filed:
October 27, 2009
Publication date:
April 29, 2010
Applicant:
Nodality, Inc. a Delaware Corporation
Inventors:
Todd Covey, Adam Palazzo, Norman Purvis, James Cordiero, David Rosen