Abstract: A method for manufacturing a high-affinity antibody including purifying an antibody to which a treatment of reducing the affinity for an antigen is not applied by a temperature responsive protein A medium, in which dissociation constant (KD value) to an antigen is smaller than the KD value of an antibody purified by an acid elution type protein A medium.

Abstract: It is an object of the present invention to provide an affinity chromatographic adsorbent using temperature-responsive protein A, wherein the adsorbent is capable of improving the culture productivity of the temperature-responsive protein A and the stability of the temperature-responsive protein A in cell disruption solution. According to the present invention, there is provided an adsorbent consisting of a carrier, to which a polypeptide comprising a tag peptide, a linker sequence, and a B-domain mutant derived from protein A from the N-terminal side thereof binds, wherein the linker sequence is an amino acid sequence that does not comprise a Val-Pro-Arg sequence and is composed of 7 to 12 amino acid residues; and the binding property of the B-domain mutant derived from protein A to an immunoglobulin can vary depending on temperature under conditions of pH 5 to 9 and a temperature of lower than 60° C.

Abstract: A method for manufacturing a carrier having temperature responsive protein A, which is protein A mutated such that a binding property to an antibody changes depending upon temperature, immobilized thereto, wherein the temperature responsive protein A has the binding property to the antibody in a first temperature region and no binding property to the antibody in a second temperature region; and the method comprises a step of immobilizing the temperature responsive protein A to a carrier surface at a temperature within the first temperature region in which the temperature responsive protein A has the binding property to the antibody.

Abstract: A mutant of the polypeptide Protein A, wherein immunoglobulin binding properties can be altered by changing temperature under the conditions of pH 5-9, below 60° C. The use of the mutant Protein A include the use thereof as a ligand coupled to an affinity chromatography support for the purification of immunoglobulins by affinity chromatography, wherein the immunoglobulins is eluted by changing temperature and thereby the conformation of the mutant Protein A.

Abstract: A mutant of the polypeptide Protein A, wherein immunoglobulin binding properties can be altered by changing temperature under the conditions of pH 5-9, below 60° C. The use of the mutant Protein A include the use thereof as a ligand coupled to an affinity chromatography support for the purification of immunoglobulins by affinity chromatography, wherein the immunoglobulins is eluted by changing temperature and thereby the conformation of the mutant Protein A.

Abstract: A mutant of the polypeptide Protein A, wherein immunoglobulin binding properties can be altered by changing temperature under the conditions of pH 5-9, below 60° C. The use of the mutant Protein A include the use thereof as a ligand coupled to an affinity chromatography support for the purification of immunoglobulins by affinity chromatography, wherein the immunoglobulins is eluted by changing temperature and thereby the conformation of the mutant Protein A.

Abstract: [Object] The aim of the present invention is to develop a novel immunoglobulin binding polypeptide, the loss and formation of whose native three dimensional structure can be controlled under the conditions where unwanted effects such as damages, loss of functions or unintended functions do not occur to immunoglobulin (pH 5-9, lower than 60° C.), and use thereof as a ligand coupled to an affinity chromatography support. [Means for solving the problems] A mutant of the polypeptide set forth in SEQ ID NO 1 or SEQ ID NO 2, wherein immunoglobulin binding properties can be altered by changing temperature under the conditions of pH 5-9, below 60° C., and the use thereof.