Abstract: The methods of the present invention include noninvasive methods for the isolation of antibody-producing cells from the gut and the use of the cells so-isolated to generate antibodies responsive to biomarkers for a number of health conditions. The responsive antibodies are chimeric secretory antibodies comprising IgA and IgG moieties that may be useful in the diagnosis and treatment of various health conditions after challenged with biomarkers thereof. In a preferred embodiment, stool samples may be obtained from patients suffering from HIV infection and cells may be isolated from the samples according to the methods described herein and reacted with antibodies responsive to HIV biomarkers. The diagnostic methods described herein allow for room temperature sample isolation for up to five days prior to diagnosis and are useful in detecting latent HIV that cannot be detected in blood samples. It is another object of the invention to generate therapeutic antibodies.