Abstract: Methods of diagnosis or of quantitation of pathological conditions comprise conducting an immunoassay to measure neo-epitope containing protein fragments naturally present in a biofluid sample, and associating an elevation of said measure in said patient above a normal level with the presence or extent of pathology. The immunoassay is conducted by a method comprising: contacting protein fragments naturally present in said sample with an immunological binding partner reactive with a neo-epitope formed by cleavage of a protein by a proteinase and measuring the extent of binding of peptide fragments to said immunological binding partner to measure therein protein fragments comprising said neo-epitope. Neo-epitopes from, collagen type I, collagen type III, collagen type IV, collagen type V, collagen type VI, elastin, biglycan, decorin, lumican, versican, C-reactive protein, ApoE and laminins are described.
Type:
Application
Filed:
July 20, 2011
Publication date:
February 23, 2012
Applicant:
Nordic Bioscience A/S
Inventors:
Sanne S. Veidal, Morten A. Karsdal, Diana J. Leeming, Natasha Barascuk, Helene Skjøt-Arkil, Efstathios Vassiliadis
Abstract: A method of immunoassay for fragments of a protein such as type II collagen in a biological sample detects fragments having a first epitope containing an isomerised amino acid residue and a second epitope generated by cleavage of the protein by the use of respective antibodies binding each of the two epitopes.
Type:
Application
Filed:
November 11, 2009
Publication date:
October 20, 2011
Applicant:
Nordic Biosciences A/S
Inventors:
Diana J. Leeming, Inger Byrjalsen, Per Qvist, Morten A. Karsdal
Abstract: A method of deriving a quantitative measure of a degree of calcification of a blood vessel such as an aorta by processing an image such as an X-ray image of at least a part of the blood vessel containing said calcification comprises: taking a starting set of digital data representative of an image of at least part of a blood vessel containing a calcification set against a background; estimating the boundary of the calcification; using inpainting to replace digital data in said starting set representing the calcification with data extrapolating the boundary of the background to extend over the area of calcification, and so generating an inpainted set of digital data; and computing the difference between the starting set of digital data and the inpainted set of digital data to obtain a quantitative measure of the degree of calcification of the blood vessel.
Type:
Application
Filed:
June 2, 2006
Publication date:
August 27, 2009
Applicant:
Nordic Bioscience A/S
Inventors:
Marleen De Bruijne, Mads Nielsen, Claus Christiansen, Lars A. Conrad-Hansen, Francois B. Lauze
Abstract: A method for the analysis of three dimensional scan data representing an articular cartilage is provided to extract a quantitative parameter indicative of joint pathology. A measure of local curvature of the cartilage is determined within a region of interest. The value of the quantitative parameter of this joint derived from this measure is compared with the value of a similar quantitative parameter previously established in respect of healthy joints and/or joints characterised by a pathology.
Type:
Application
Filed:
September 4, 2006
Publication date:
July 30, 2009
Applicant:
Nordic Bioscience A/S
Inventors:
Erik B. Dam, Jenny Folkesson, Paola Petterson, Ole F. Olsen, Mads Nielsen, Claus Christiansen