Abstract: A combination of gonadotrophins and growth hormone is useful for treating infertility in higher mammals and humans since the probability of pregnancy is enhanced. In the treatment, gonadotrophins and growth hormone are injected separately, or a combined preparation containing both of these active substances may be used.

Abstract: A DNA sequence containing a ribosome binding site with a SD sequence AGGA and a start codon ATG, comprising a nucleotide sequence having the formula Y.sub.1 . . . Y.sub.m ZVAGGA X.sub.1, X.sub.2 . . . X.sub.n ATG wherein Y.sub.1 . . . Y.sub.m represents a promoter with one or more restriction enzyme sites, m being an integer corresponding to the number of base pairs in the promoter, each X, Y, Z and V is A, T, C or G; n is 9 to 13, and the sequence X.sub.1 . . . X.sub.n cannot contain a start codon.

Abstract: Methods and compositions are provided for recombinant DNA production of Factor VIIIC and truncated derivatives therfeof. Based on amino acid sequences, probes are developed for isolating messenger RNA and/or chromosomal DNA encoding for Factor VIIIC. The Factor VIIIC gene in its entirety or encoding for a fragment thereof is then used for expression of Factor VIIIC in a host.The bacteriophage .lambda.FVIII23D containing the 14.43kb EcoRI fragment was deposited at the A.T.C.C. on Jan. 4, 1984 and given Accession No. 40094.

Abstract: An injection apparatus has a jacket having a front end and a cartridge for containing a liquid having a rubber membrane closing a front end of the cartridge at the front end of the jacket. A double-ended needle is held in a needle holder such that both ends thereof are exposed and oriented, one end at the membrane for piercing the membrane to obtain the liquid from the cartridge, and the opposite end for injecting the liquid. First cooperative threads on a cap and the jacket are for screwing the cap onto the jacket and unscrewing the cap therefrom, the first cooperative threads having a first pitch and a first rotational hand.

Abstract: Hybridomas producing monoclonal antibodies specific for polypeptide fragments derived from human Factor VIIIC are provided. Class I hybridomas produce monoclonal antibodies reactive with a 80/77 kd doublet fragment or with both the 80/77 kd doublet and a 240 kd polypeptide. Class III hybridomas produce monoclonal antibodies reactive with the 240 kd polypeptide as well as a 92.5 kd fragment and its precursors. Class III antibodies show additional reactivity with a 40 kd thrombin digestion product. The monoclonal antibodies are useful for the separation of Factor VIIIC and its constituent polypeptides, as well as for the immunoassay of Factor VIIIC in biological samples.

Abstract: Production of a high purity concentrate of the antihemophilic Factor VIII (AHF) by precipitation of an aqueous solution of cryoprecipitate from blood plasma in a first step with such an amount of polyethylene glycol (PEG), preferably about 4% by weight, as will precipitate a substantial amount of the present fibrinogen, subjecting the fibrinogen-free solution to a second precipitation step with preferably about 12% by weight of PEG in the presence of a salting-in agent, such as an amino acid, in particular lysine or arginine, or a carbohydrate, and then recovering the precipitate with a concentrated content of the present Factor VIII. The obtained Factor VIII concentrate with a very low content of immunoglobulins and other plasma proteins has a solubility in an aqueous injection medium of 45 to 500 units/ml and a high specific activity of up to 50 units/mg protein.