Patents Assigned to Norgen Biotek Corp
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Patent number: 11767522Abstract: Provided is a composition for preserving cell-free nucleic acids and/or cells in a biological sample and methods for use thereof. The composition comprises at least one volume excluding polymer, at least one osmotic agent and at least one enzyme inhibitor. The composition optionally further comprises at least one metabolic inhibitor. Further, provided is a kit comprising the composition, preferably in a blood collection tube, or the components of the composition.Type: GrantFiled: May 16, 2017Date of Patent: September 26, 2023Assignee: Norgen Biotek Corp.Inventors: Taha Alexander Haj-Ahmad, Yousef Haj-Ahmad
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Publication number: 20220090169Abstract: Disclosed are methods and kits for improving global gene expression analysis for a population of RNA molecules derived from a human urine sample. In an embodiment, the method comprises the step of selectively depleting miR-10a-5p fragments from the population of RNA molecules or selectively blocking miR-10a-5p fragments within the RNA population. The miR-10a-5p depleted or miR-10a-5p blocked population of RNA can be used in a variety of global gene expression analysis protocols, including next generation sequencing. In a further embodiment, the method comprises selectively depleting or blocking miR-10b-5p fragments within the RNA population. The miR-10a-5p and/or miR-10b-5p depleted or blocked populations of RNA can also be used in global gene expression analysis protocols, including next generation sequencing.Type: ApplicationFiled: December 8, 2021Publication date: March 24, 2022Applicant: Norgen Biotek Corp.Inventor: Yousef Haj-Ahmad
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Publication number: 20220080404Abstract: An apparatus for collecting and combining a sample, and in particular, a biological sample, with a reagent, and uses thereof. The apparatus comprises a sample collection container and a complementary sealing cap. A sealed reagent reservoir containing a reagent, such as a preservative reagent, is provided in a lower portion of the collection container. A piercing insert is nested within the collection container, above the seal of the reagent reservoir. Following the collection of the sample into the collection container, the action of sealing the collection container with the cap causes the piercing insert to engage with and disrupt the seal on the reagent reservoir, thereby exposing the collected sample to the reagent.Type: ApplicationFiled: September 14, 2021Publication date: March 17, 2022Applicant: Norgen Biotek Corp.Inventor: Yousef Haj-Ahmad
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Patent number: 11208678Abstract: Disclosed are methods and kits for improving global gene expression analysis for a population of RNA molecules derived from a human urine sample. In an embodiment, the method comprises the step of selectively depleting miR-10a-5p fragments from the population of RNA molecules or selectively blocking miR-10a-5p fragments within the RNA population. The miR-10a-5p depleted or miR-10a-5p blocked population of RNA can be used in a variety of global gene expression analysis protocols, including next generation sequencing. In a further embodiment, the method comprises selectively depleting or blocking miR-10b-5p fragments within the RNA population. The miR-10a-5p and/or miR-10b-5p depleted or blocked populations of RNA can also be used in global gene expression analysis protocols, including next generation sequencing.Type: GrantFiled: July 23, 2018Date of Patent: December 28, 2021Assignee: Norgen Biotek Corp.Inventor: Yousef Haj-Ahmad
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Patent number: 10876105Abstract: Disclosed is a method for the isolation of extracellular vesicles, including exosomes, from a liquid sample, the method comprising the steps of: adjusting the pH of a liquid sample comprising extracellular vesicles to a preselected, binding pH; contacting the liquid sample with silicon carbide, wherein at the preselected, binding pH, the extracellular vesicles bind to the silicon carbide; and eluting the bound extracellular vesicles from the silicon carbide. The liquid samples can comprise bodily fluids. Further disclosed is a method for producing a liquid sample, substantially depleted of extracellular vesicles, including exosomes.Type: GrantFiled: November 14, 2018Date of Patent: December 29, 2020Assignee: Norgen Biotek Corp.Inventor: Yousef Haj-Ahmad
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Publication number: 20200224189Abstract: Provided is a composition for preserving cell-free nucleic acids and/or cells in a biological sample and methods for use thereof. The composition comprises at least one volume excluding polymer, at least one osmotic agent and at least one enzyme inhibitor. The composition optionally further comprises at least one metabolic inhibitor. Further, provided is a kit comprising the composition, preferably in a blood collection tube, or the components of the composition.Type: ApplicationFiled: May 16, 2017Publication date: July 16, 2020Applicant: Norgen Biotek Corp.Inventors: Taha Alexander HAJ-AHMAD, Yousef HAJ-AHMAD
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Patent number: 10577645Abstract: Disclosed are methods and kits for improving global gene expression analysis for a population of RNA molecules derived from a human blood, plasma and/or serum sample. In an embodiment, the method comprises the step of selectively depleting 5?-RNAY4 fragments from the population of RNA molecules or selectively blocking 5?-RNAY4 fragments within the RNA population. The 5?-RNAY4 depleted or 5?-RNAY4 blocked population of RNA can be used in a variety of global gene expression analysis protocols, including next generation sequencing. In a further embodiment, the method comprises selectively depleting or blocking miR-486-5p fragments within the RNA population. The miR-486-5p depleted or miR-486-5p blocked population of RNA can also be used in global gene expression analysis protocols, including next generation sequencing.Type: GrantFiled: March 16, 2017Date of Patent: March 3, 2020Assignee: Norgen Biotek Corp.Inventor: Yousef Haj-Ahmad
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Publication number: 20200024642Abstract: Disclosed are methods and kits for improving global gene expression analysis for a population of RNA molecules derived from a human urine sample. In an embodiment, the method comprises the step of selectively depleting miR-10a-5p fragments from the population of RNA molecules or selectively blocking miR-10a-5p fragments within the RNA population. The miR-10a-5p depleted or miR-10a-5p blocked population of RNA can be used in a variety of global gene expression analysis protocols, including next generation sequencing. In a further embodiment, the method comprises selectively depleting or blocking miR-10b-5p fragments within the RNA population. The miR-10a-5p and/or miR-10b-5p depleted or blocked populations of RNA can also be used in global gene expression analysis protocols, including next generation sequencing.Type: ApplicationFiled: July 23, 2018Publication date: January 23, 2020Applicant: Norgen Biotek Corp.Inventor: Yousef Haj-Ahmad
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Patent number: 10287625Abstract: Disclosed are methods and kits for isolating nucleic acids having a size above a desired cut-off size from a nucleic acid containing sample. The method comprises combining the sample with a binding buffer, alcohol and silicon carbide to provide a binding mixture. Nucleic acids having a size above the desired cut-off size are selectively bound to the silicon carbide. The cut-off size for selective binding to the silicon carbide is determined by the alcohol concentration of the binding mixture. The bound nucleic acids are separated from the remaining sample. The bound nucleic acids are optionally washed and then eluted from the silicon carbide. The kit comprises a buffer binding to be diluted with alcohol to provide an alcohol concentration of about 1 to about 50% (v/v), a wash solution, an elution solution, silicon carbide and instructions for adjusting the alcohol concentration to selectively bind nucleic acids having a size above the desired cut-off size.Type: GrantFiled: March 16, 2017Date of Patent: May 14, 2019Assignee: Norgen Biotek Corp.Inventor: Yousef Haj-Ahmad
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Publication number: 20190078078Abstract: Disclosed is a method for the isolation of extracellular vesicles, including exosomes, from a liquid sample, the method comprising the steps of: adjusting the pH of a liquid sample comprising extracellular vesicles to a preselected, binding pH; contacting the liquid sample with silicon carbide, wherein at the preselected, binding pH, the extracellular vesicles bind to the silicon carbide; and eluting the bound extracellular vesicles from the silicon carbide. The liquid samples can comprise bodily fluids. Further disclosed is a method for producing a liquid sample, substantially depleted of extracellular vesicles, including exosomes.Type: ApplicationFiled: November 14, 2018Publication date: March 14, 2019Applicant: Norgen Biotek Corp.Inventor: Yousef Haj-Ahmad
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Patent number: 10160964Abstract: Disclosed is a method for the isolation of extracellular vesicles, including exosomes, from a liquid sample, the method comprising the steps of: adjusting the pH of a liquid sample comprising extracellular vesicles to a preselected, binding pH; contacting the liquid sample with silicon carbide, wherein at the preselected, binding pH, the extracellular vesicles bind to the silicon carbide; and eluting the bound extracellular vesicles from the silicon carbide. The liquid samples can comprise bodily fluids. Further disclosed is a method for producing a liquid sample, substantially depleted of extracellular vesicles, including exosomes.Type: GrantFiled: May 6, 2016Date of Patent: December 25, 2018Assignee: Norgen Biotek Corp.Inventor: Yousef Haj-Ahmad
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Patent number: 9845463Abstract: Provided are methods and columns employing a solid support comprising silica and silicon carbide for the isolation and purification of nucleic acids, and in particular, the isolation and purification of both high and low molecular weight RNA.Type: GrantFiled: July 19, 2016Date of Patent: December 19, 2017Assignee: NORGEN BIOTEK CORP.Inventor: Yousef Haj-Ahmad
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Publication number: 20170268040Abstract: Disclosed are methods and kits for improving global gene expression analysis for a population of RNA molecules derived from a human blood, plasma and/or serum sample. In an embodiment, the method comprises the step of selectively depleting 5?-RNAY4 fragments from the population of RNA molecules or selectively blocking 5?-RNAY4 fragments within the RNA population. The 5?-RNAY4 depleted or 5?-RNAY4 blocked population of RNA can be used in a variety of global gene expression analysis protocols, including next generation sequencing. In a further embodiment, the method comprises selectively depleting or blocking miR-486-5p fragments within the RNA population. The miR-486-5p depleted or miR-486-5p blocked population of RNA can also be used in global gene expression analysis protocols, including next generation sequencing.Type: ApplicationFiled: March 16, 2017Publication date: September 21, 2017Applicant: Norgen Biotek Corp.Inventor: Yousef Haj-Ahmad
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Publication number: 20170268047Abstract: Disclosed are methods and kits for isolating nucleic acids having a size above a desired cut-off size from a nucleic acid containing sample. The method comprises combining the sample with a binding buffer, alcohol and silicon carbide to provide a binding mixture. Nucleic acids having a size above the desired cut-off size are selectively bound to the silicon carbide. The cut-off size for selective binding to the silicon carbide is determined by the alcohol concentration of the binding mixture. The bound nucleic acids are separated from the remaining sample. The bound nucleic acids are optionally washed and then eluted from the silicon carbide. The kit comprises a buffer binding to be diluted with alcohol to provide an alcohol concentration of about 1 to about 50% (v/v), a wash solution, an elution solution, silicon carbide and instructions for adjusting the alcohol concentration to selectively bind nucleic acids having a size above the desired cut-off size.Type: ApplicationFiled: March 16, 2017Publication date: September 21, 2017Applicant: Norgen Biotek Corp.Inventor: Yousef Haj-Ahmad
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Publication number: 20160333338Abstract: Disclosed is a method for the isolation of extracellular vesicles, including exosomes, from a liquid sample, the method comprising the steps of: adjusting the pH of a liquid sample comprising extracellular vesicles to a preselected, binding pH; contacting the liquid sample with silicon carbide, wherein at the preselected, binding pH, the extracellular vesicles bind to the silicon carbide; and eluting the bound extracellular vesicles from the silicon carbide. The liquid samples can comprise bodily fluids. Further disclosed is a method for producing a liquid sample, substantially depleted of extracellular vesicles, including exosomes.Type: ApplicationFiled: May 6, 2016Publication date: November 17, 2016Applicant: Norgen Biotek Corp.Inventor: Yousef Haj-Ahmad
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Publication number: 20160326512Abstract: Provided are methods and columns employing a solid support comprising silica and silicon carbide for the isolation and purification of nucleic acids, and in particular, the isolation and purification of both high and low molecular weight RNA.Type: ApplicationFiled: July 19, 2016Publication date: November 10, 2016Applicant: Norgen Biotek Corp.Inventor: Yousef Haj-Ahmad
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Patent number: 9422596Abstract: Provided are methods and columns employing a solid support comprising silica and silicon carbide for the isolation and purification of nucleic acids, and in particular, the isolation and purification of both high and low molecular weight RNA.Type: GrantFiled: April 27, 2015Date of Patent: August 23, 2016Assignee: Norgen Biotek Corp.Inventor: Yousef Haj-Ahmad
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Publication number: 20140255271Abstract: The present invention provides a device for the concentration of one or more target analytes contained in a urine sample. The device comprises a tube comprising an upper portion defining an opening for receiving the urine sample and a lower tapered portion terminating in a collection reservoir. The tube contains a predetermined amount of a particulate binding agent which specifically binds the one or more target analytes and of a predetermined amount of a binding buffer. The device comprises means for sealing the opening of the tube. The present invention further provides methods and kits for concentrating one or more target analytes in a urine sample.Type: ApplicationFiled: April 30, 2014Publication date: September 11, 2014Applicant: Norgen Biotek Corp.Inventor: Yousef Haj-Ahmad
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Publication number: 20130149691Abstract: Provided is a nucleic acid preservative comprising at least one reducing agent, at least one chaotropic substance, at least one polyamine substance and at least one chelating agent and uses thereof, and a method for the preservation of nucleic acids in a biological sample. Further provided are kits for use in the preservation of nucleic acids in a biological sample, and more particularly, a blood sample.Type: ApplicationFiled: June 15, 2012Publication date: June 13, 2013Applicant: Norgen Biotek Corp.Inventor: Yousef Haj-Ahmad
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Publication number: 20120021407Abstract: The present invention provides a device for the concentration of one or more target analytes contained in a urine sample. The device comprises a tube comprising an upper portion defining an opening for receiving the urine sample and a lower tapered portion terminating in collection reservoir. The tube contains a predetermined amount of a particulate binding agent which specifically binds the one or more target analytes and of a predetermined amount of a binding buffer. The device comprises means for seating the opening of the tube. The present invention further provides methods and kits for concentrating one or more target analytes in murine sample.Type: ApplicationFiled: July 22, 2011Publication date: January 26, 2012Applicant: Norgen Biotek Corp.Inventor: Yousef Haj-Ahmad