Abstract: The present disclosure provides the use of BAZ1B_K426hy in the preparation of a product for tumor detection and belongs to the field of biotechnology. The present disclosure further provides a group of immunogenic polypeptides, including polypeptide A and polypeptide B. An anti-BAZ1B_K426hy polyclonal antibody is prepared by conducting mixed immunization on an animal with the immunogenic polypeptide. The polyclonal antibody can specifically recognize an endogenous protein BAZ1B_K426hy by enzyme-linked immunosorbent assay (ELISA)/Dot blot/Western blot, which is used for preparation of detection products for tumors and Williams syndrome.

Abstract: Disclosed is a stereophotogrammetric method based on binocular vision, including the following steps: image acquisition, image correction and stereo matching are performed; cost matching and cost aggregation are performed on images of different sizes after correction; image segmentation is performed on the corrected image to determine edge pixel points of the object to be measured; and a pixel distance at an edge of the object to be measured is calculated to measure the size of the object. The method of the present invention enhances the matching accuracy of contour pixels of the object to be measured and improves the measurement accuracy.

Abstract: The present disclosure relates to a fabricated concrete connection structure and a construction method, the structure including shear wall members. Tops of the shear wall members are fixedly connected to a plurality of connection female heads, and the plurality of connection female heads are arranged at equal intervals along length directions of the shear wall members. Reinforcing cage pre-formed holes penetrate through the tops and bottoms of the shear wall members. A plurality of connection male heads are arranged at equal intervals along the length directions of the shear wall members, and the plurality of connection male heads are in one-to-one correspondence with the plurality of connection female heads. One sides of the bottoms of the shear wall members are fixedly connected to folding plates. The present disclosure can achieve the purpose of connecting the shear wall members conveniently and quickly, improving the efficiency and quality of construction.

Abstract: The present invention discloses additive for reducing the roasting temperature of fluxed magnetite pellets and a method of using it, consisting of components: B2O3, Mn2O3, the B2O3 and Mn2O3 are pure chemical reagents, the mass of the additive is 0.8%, 4% of the dry basis mass of the magnetite concentrate, respectively, the magnetite concentrate, bentonite clay, calcium flux and additives will be dosed with 12-14% water of the dry base mass ratio of the mixture, prepared into green pellets of 10-12.5 mm in diameter in a disc ball making machine, After the pellets are completely dried, preheat them for 15˜20 min at 600˜1000° C. to ensure that Mn2O3 is fully decomposed, then roasting is carried out for 15 min at 1200° C., and after roasting, the pellets are cooled to room temperature to obtain the finished pellets.

Abstract: The present invention discloses a high nitrogen steel with high strength, low yield ratio and high corrosion resistance for ocean engineering, comprising the following chemical components by weight percentage: C?0.01%, Si?0.1%, Cr 17%-19%, Mn 14%-16%, Mo 1%-1.5%, Ti?0.05%, N 0.45%-0.6%, P?0.01%, S?0.01%, O?0.02%, and the balance of iron. The present invention also discloses a preparation method as follows: (1) raw material weighing; (2) ingot preparation, remelting and smelting; (3) solution and forging treatments; and (4) hot rolling and post-rolling treatment. A product provided by the present invention has high tensile strength, low yield ratio and high corrosion resistance. At the same time, the present invention does not need pressurized equipment in the preparation process, therefore the preparation method is simple, the cost is low, and the present invention is suitable for industrial popularization in China.

Abstract: A drilling machine tool with a cooling liquid purifying and recycling mechanism. The drilling machine tool comprises a platform in which a blanking hole is formed, wherein air cylinders are fixedly mounted at the top of a transverse plate located above the platform, a lifting plate is mounted after the output shaft ends of the air cylinders penetrate through the transverse plate, an isolation cylinder is arranged below the lifting plate, an opening is formed in the bottom of the isolation cylinder, the bottom end of the vertical shaft slidably penetrates through the isolation cylinder and is fixedly provided with a drill bit, the isolation cylinder is connected with the lifting plate through pressing mechanisms, and a cooling liquid conveying mechanism communicating with the interior of the isolation cylinder is arranged on the platform; and a cooling liquid purifying and recycling mechanism is arranged at the bottom of the platform.

Abstract: The present invention discloses a high nitrogen steel with high strength, low yield ratio and high corrosion resistance for ocean engineering, comprising the following chemical components by weight percentage: C?0.01%, Si?0.1%, Cr 17%-19%, Mn 14%-16%, Mo 1%-1.5%, Ti?0.05%, N 0.45%-0.6%, P?0.01%, S?0.01%, O?0.02%, and the balance of iron. The present invention also discloses a preparation method as follows: (1) raw material weighing; (2) ingot preparation, remelting and smelting; (3) solution and forging treatments; and (4) hot rolling and post-rolling treatment. A product provided by the present invention has high tensile strength, low yield ratio and high corrosion resistance. At the same time, the present invention does not need pressurized equipment in the preparation process, therefore the preparation method is simple, the cost is low, and the present invention is suitable for industrial popularization in China.

Abstract: The present invention provides a recombinant adipose-derived stem cell and a recombinant method thereof, and belongs to the technical field of genetic engineering, where an adenovirus carrying an hFIX gene is transfected into an adipose-derived stem cell to obtain the recombinant adipose-derived stem cell. In the present invention, an adenovirus carrying an hFIX gene is transfected into an adipose-derived stem cell, and the recombinant adipose-derived stem cell obtained after the transfection can express an hFIX protein.

Abstract: A protein assay method, including: 1) dissolving 4-hydroxybenzenesulfonic acid in an ethanol, methanol, or acetonitrile aqueous solution to prepare a hydrolysis reagent, adding a protein sample and the hydrolysis reagent to a hydrolysis tube, and uniformly mixing the protein sample and the hydrolysis reagent, charging argon or nitrogen into the hydrolysis tube to remove dissolved oxygen, sealing and drying the hydrolysis tube, and hydrolyzing the protein sample; 2) cooling the hydrolysis tube to room temperature, filtering and adding a resulting hydrolysate to a volumetric flask, washing the hydrolysis tube and a filter paper, collecting and adding a resulting washing solution to the volumetric flask to a constant volume; 3) drawing a hydrolysate solution from the volumetric flask, completely drying the hydrolysate solution to yield a solid product; and 4) diluting the solid product using a diluent, and analyzing amino acids of the solid product.

Abstract: The present invention relates to a mineral processing technology and method for refractory chalcopyrite ores, particularly to a mineral processing technology and method for the separation of chalcopyrite from multiple natural types of copper ores containing chalcopyrite, pyrrhotite, talc and serpentine, which belongs to the technical field of mineral processing. It's characterized by: conducting a two-stage grinding on the chalcopyrite ore, with each grinding stage followed by size grading, and treating ores in different size fractions separately, wherein coarse-grain ores are separated in the presence of xanthate, with calcium oxide and CMC controlling the pH and acting as depressor respectively, while fine-grained ores are subjected to rougher in the presence of kerosene, and subsequently subjected to cleaner in the presence of xanthate.