Abstract: A window burner for creating windows by selective removal of polymer type coatings from capillary columns and very small diameter tubes. The burner includes an electric circuit which provides a predetermined, controlled current from a standard 120 or 220 volt AC outlet. The predetermined, controlled current electrically heats a resistive wire for a predetermined period of time. The resistive wire may have a predetermined configuration to facilitate mounting of the capillary column for selective burning. The size of the resistive wire and the predetermined period of time during which the predetermined, controlled current is provided thereto are factors determining the size of the window formed by selectively burning off the polymer coating.

Abstract: Enzyme amplification is achieved by covalently bonding enzyme to a supporting material via a molecular chain which is a substrate for the enzyme, then introducing a small amount of enzyme in the free state to this system, causing release of a large amount of bound enzyme. In an alternative embodiment, complementary enzymatically inactive fragments of an active enzyme, which fragments can recombine to form active enzyme, are covalently attached to separate support materials by a molecular chain material which is a substrate for the active enzyme, and these two fragment-support conjugates are connected in series. Upon application of free enzyme or free complementary enzyme to one of these fragment-support conjugates, followed by application of the resulting product mixture to the second fragment-support conjugate, a large amount of free enzyme is ultimately produced.

Abstract: A method for forming a single-phase, homogeneous and high surface area metal alloy by reducing a polyheterometallic complex at a low temperature in hydrogen-containing gas.

Abstract: An integrated temperature control/alignment system for use with a high performance capillary electrophoretic apparatus is disclosed. In one exemplary embodiment the integrated temperature control/alignment system comprises a pair of capillary column mounting plates for mounting a capillary column as a constituent of the electrophoretic apparatus, a pair of secondary support plates, a pair of thermoelectric plates and external heat sink plates. Detection openings are formed in each of the plates as required so that a continuous detection path exists through the integrated temperature control/alignment system. The capillary column is seated and locked in grooves formed in the capillary column mounting plates so that the detection windows of the capillary column are coordinated with the aligned detection openings formed in the applicable plates of the integrated temperature control/alignment system.

Abstract: A microcapillary column for high performance electrophoresis includes a fused silica microcapillary, a gel of crosslinked polyacrylamide polymerized in the tube, and a thin layer of connecting material covalently bonded to the inner surface of the microcapillary wall and to the polymeric gel. The gel-containing microcapillary is prepared by first covalently bonding a suitable bifunctional reagent to the inner surface of the microcapillary wall, and then causing a mixture of monomer, crosslinking agent, and polymerization catalyst to react in the bore of the microcapillary to form a polymeric matrix which is covalently bonded to the microcapillary wall via the bifunctional reagent. In electrophoresis, the gel-containing microcapillary provides peak efficiencies in excess of 100,000 theoretical plates within separation times of less than thirty minutes, and permits trace level determinations of molecular weights.

Abstract: An improved microcapillary column for high performance electrophoresis includes a microcapillary, a hydrophilic polymer within a gel of crosslinked polyacrylamide polymerized in the tube, and preferably, a thin layer of connecting material covalently bonded to the inner surface of the microcapillary wall and to the polymeric gel. The microcapillary is prepared by first covalently bonding a suitable bifunctional reagent to the inner surface of the microcapillary wall, and then causing a mixture of the hydrophilic polymer, monomer, crosslinking agent, and polymerization catalyst to react in the bore of the microcapillary to form a hydrophilic polymer-containing gel matrix which is covalently bonded to the microcapillary wall via the bifunctional reagent.

Abstract: A repetitive immunoassay analytical method for determination of a free analyte is carried out by loading an affinity column of covalently bound analyte with tagged antibody, passing a continuous aqueous stream of carrier liquid over the column, introducing an aliquot of a sample to be analyzed for free analyte into the carrier stream upstream of the column, and monitoring the eluting carrier stream for a signal spike resulting from the presence of tagged antibody material released from the column by the application of free analyte in the analytical sample. Many samples may be analyzed by this method before the antibody-loaded affinity column needs to be regenerated. It is also disclosed that substrate-analyte conjugates of superior stability are produced by linking a substrate to a hydroxyalkyl analyte via an amino, hydrazide, or sulfide linking group replacing a hydroxy group.

Abstract: Fast high resolution separations of biopolymers with retention of biological activity have been achieved by hydrophobic interaction chromatography using trialkoxy silyl ethers of the general formula(RO).sub.3 Si--(CH.sub.2).sub.m --O--(CH.sub.2 CH.sub.2 O).sub.n --(CH.sub.2).sub.p R'chemically bonded to silica-based chromatographic supports. In the formula R is alkyl of from one to five carbons, m is an integer from two to five, n is an integer from one to five, p is an integer from zero of ten, and R' is methyl, phenyl, or substituted phenyl. Stable and reproducible bonded phases are prepared in a novel solventless procedure by a bonding process which uses a defined and controlled amount of water on the silica surface and a gaseous or volatile basic catalyst such as ammonia to produce a controlled amount of silane polymerization and cross-linking in addition to extensive bonding between silane and silica.

Abstract: A new class of chemical reagents called release tags which comprise signal, release and reactivity groups is disclosed and a release tag involving a pentafluorobenzoyl signal group, a methionylamide release group, and an active ester reactivity group is used to analyze the hormone, thyroxine, in serum, involving quantitation of the released signal group by gas chromatography with electron capture detection.

Abstract: Coal liquid fractions to be used as fuels are stabilized against gum formation and viscosity increases during storage, permitting the fuel to be burned as is, without further expensive treatments to remove gums or gum-forming materials. Stabilization is accomplished by addition of cyclohexanol or other simple inexpensive secondary and tertiary alcohols, secondary and tertiary amines, and ketones to such coal liquids at levels of 5-25% by weight with respect to the coal liquid being treated. Cyclohexanol is a particularly effective and cost-efficient stabilizer. Other stabilizers are isopropanol, diphenylmethanol, tertiary butanol, dipropylamine, triethylamine, diphenylamine, ethylmethylketone, cyclohexanone, methylphenylketone, and benzophenone. Experimental data indicate that stabilization is achieved by breaking hydrogen bonds between phenols in the coal liquid, thereby preventing or retarding oxidative coupling.

Abstract: Antidopaminergic agents having selective limbic activity, comprising (S)-enantiomers of aporphine compounds of either the following structures: ##STR1## wherein R.sub.1 is lower alkyl, alkyl substituted lower alkyl, alkyl substituted cycloalkyl, lower alkenyl, alkyl substituted lower alkenyl, lower alkynyl, alkyl substituted lower alkynyl, phenyl lower alkyl, phenyl lower alkenyl, and phenyl lower alkynyl, and the following structure where R.sub.1 is above and R.sub.2 and R.sub.3 are hydrogen, methyl, or R.sub.1 ##STR2## R.sub.1 is as above R.sub.4 is H, CH.sub.3 lower alkyl, or R.sup.1 C0--where R.sup.1 =CH.sub.3 or lower alkyl.

Abstract: Coal liquid fractions to be used as fuels are stabilized against gum formation and viscosity increases during storage, permitting the fuel to be burned as is, without further expensive treatments to remove gums or gum-forming materials. Stabilization is accomplished by addition of cyclohexanol or other simple inexpensive secondary and tertiary alcohols, secondary and tertiary amines, and ketones to such coal liquids at levels of 5-25% by weight with respect to the coal liquid being treated. Cyclohexanol is a particularly effective and cost-efficient stabilizer. Other stabilizers are isopropanol, diphenylmethanol, tertiary butanol, dipropylamine, triethylamine, diphenylamine, ethylmethylketone, cyclohexanone, methylphenylketone, and benzophenone. Experimental data indicate that stabilization is achieved by breaking hydrogen bonds between phenols in the coal liquid, thereby preventing or retarding oxidative coupling.

Abstract: Cold-hearth melt-spinning apparatus for adapted improved continuous casting of refractory and reactive alloys possesses (1) a cold crucible having a selectively grooved surface operative to reduce both melt "freezing" at the melt/crucible interface and input heating power requirements, and (2) a replaceable nozzle of a refractory material that is friction-fit in an aperture provided therefor in the crucible. The confronting nozzle/crucible walls define a thermally impeding interface which maintains the nozzle at a temperature sufficient to substantially eliminate melt "freezing" in and across the nozzle orifice. The melt is ejected as a molten jet from the crucible by pressure, rapidly quenched on a suitably configured spinning disk into either filaments or flakes, and passed to an environmentally protected processing compartment. Arc heating of the melt is provided by a water-cooled and selectively movable electrode.

Abstract: A method for providing orally effective aporphine compounds, and new compounds which are orally effective in the prevention and treatment of duodenal ulcers and in the treatment of neurological and psychiatric disorders having the following formula ##STR1## wherein R.sub.1 is lower alkyl, substituted lower alkyl, cycloalkyl, substituted cycloalkyl, lower alkenyl, substituted lower alkenyl, lower alkynyl, substituted lower alkynyl, phenyl lower alkyl, phenyl lower alkenyl and phenyl lower alkynyl, R.sub.2 and R.sub.3 are hydrogen, methyl, lower alkyl, substituted lower alkyl, cycloalkyl, substituted cycloalkyl, lower alkenyl, substituted lower alkenyl, lower alkynyl, substituted lower alkynyl, phenyl lowerThe invention described herein was made in the course of work under a grant or award from the Department of Health and Human Services.

Abstract: Rapid solidification processing by liquid quenching is made practical for multiphase alloys having relatively large liquidus-solidus temperature intervals by a new processing technique termed "melt mix reaction" involving chemically reacting two starting alloys in a mixing nozzle in which a melt mix reaction takes place between the chemically reactable components of the starting alloys to form submicron particles of the resultant compound in the final alloy. The mixing and chemical reaction is performed at a temperature which is at or above the highest liquidus temperature of the starting alloys but which is also substantially below the liquidus temperature of the final alloy, and as close to the solidus temperature of the final alloy as possible.

Abstract: Small amounts of rare earth elements are added to an .alpha. or near-.alpha. alloy of titanium and IIIA, IVA metals to provide a rare earth/IIIA, IVA metal precipitate during an age hardening heat treatment after rapid solidification of the alloy. Age hardening of the rapidly solidified alloy of .alpha. or near .alpha.-titanium creates a fine, uniform, equidistant, high density, spherical 50-100 Angstrom precipitate of the resulting rare earth/IIIA, IVA metal compound to produce a precipitate-hardened allow with twice the strength of the alloy without rare earth additives. The precipitate hardening which predominates the other hardening processes occurs during the heat treating step after rapid solidification, with the quenching associated with the rapid solidification producing solid solution hardening.

Abstract: N-substituted haloalkyl apomorphines of the formula ##STR1## wherein R.sub.1 is hydrogen or R.sub.2 O; R.sub.2 is hydrogen, acyl, lower alkyl or ##STR2## R.sub.3 and R.sub.4 are lower alkyl; and X is halogen; and their acid addition salts. Representative compounds have neuroleptic properties.