Abstract: A sterile aqueous specimen collection fluid containing a buffer, an aliphatic aldehyde at a concentration of 0.15M to 3.4M, and one or more heavy metal salts at a concentration of 0.2×10−3M to 0.2M, and having a pH of 6.8 to 8.0. Also disclosed is a method of colleting a specimen with such a fluid for haematological analysis.
Type:
Grant
Filed:
April 26, 1999
Date of Patent:
June 17, 2003
Assignees:
Northern General Hospital, Central Sheffield University Hospitals NHS Trust Royal
Hallamshire Hospital
Abstract: The invention is directed to novel methods of stabilizing blood compositions for use in the quality control of analytical techniques. The novel method comprises removing leucocytes from a blood composition to yield a depleted blood composition, stabilizing the leucocytes by treatment with an effective amount of a stabilizing agent comprising an aged transition metal solution, and then adding the resultant stabilized leucocytes to the depleted blood composition or a second blood component composition.
Type:
Grant
Filed:
June 24, 1996
Date of Patent:
March 6, 2001
Assignee:
Northern General Hospital N.H.S. Trust
Inventors:
Vivian Granger, David Barnett, John T. Reilly, Petra S. M. Mayr
Abstract: The invention is directed to novel stabilized blood compositions and methods of stabilizing blood compositions for use in the quality control of analytical techniques. The novel blood compositions comprise leucocytes and an effective amount of an aged transition metal solution. The novel method comprises removing leucocytes from a blood composition to yield a depleted blood composition, stabilizing the leucocytes by treatment with an effective amount of a stabilizing agent comprising an aged transition metal solution, and then, adding the resultant stabilized leucocytes to the depleted blood composition or a second blood component composition.
Type:
Grant
Filed:
June 21, 1996
Date of Patent:
March 6, 2001
Assignee:
Northern General Hospital N.H.S. Trust
Inventors:
Vivian Granger, David Barnett, John T. Reilly, Petra S. M. Mayr, Shawn P. Fay
Abstract: A stabilized cell suspension is prepared by treating the cell suspension with a solution of a heavy metal compound, which has been aged at pH 6.5-7.5 prior to use to allow a precipitate to form, removing the precipitate from the heavy metal solution, mixing the aged heavy metal solution with the cell suspension to form a first mixture, and mixing the first mixture with a paraformaldehyde solution to form a stabilized cell suspension which is still capable of lysis.