Abstract: A method for preparing a cell suspension for analysis includes at least: taking a sample of the cell suspension using a pipetting-dispensing device, which includes at least one pipe, and depositing the sample present in the pipetting-dispensing device in an analysis container. Between the taking of the sample and the depositing, the method includes at least one pre-analysis of the sample, which is carried out by a pre-analysis device placed on the pipe of the pipetting-dispensing device.

Abstract: The present invention relates to a process for preparing and analyzing a plurality of cell suspensions (5) comprising at least the following successive steps: (a) loading a plurality of bottles (4) onto a reception plate (6), each bottle (4) comprising a cell suspension (5) to be analyzed; (b) loading a plurality of analysis containers (32, 34, 36) onto the reception plate (6); and (c) taking a sample of a cell suspension (5) from a bottle (4) and depositing this sample in an analysis container (34, 36); wherein step (c) is repeated for each bottle (4) to be analyzed. Step (c) of taking a sample of a cell suspension from a bottle (4) and depositing this sample in an analysis container (34, 36) comprises at least one step of breaking up the cell clusters by virtue of pipetting distribution means (20). The invention also relates to an automated device for implementing this process.

Abstract: A system for depositing cells on an analysis plate, the cells being contained in a cell suspension including a fixing agent and the cells, included, for receiving the suspension, a chamber (5) which is positioned above the analysis plate and whose base is open and extends opposite a cell depositing zone of the plate and a material for absorbing the fixing agent placed around the chamber and the depositing zone of the plate. The chamber is mounted so as to be able to be moved between a first position in abutment against the analysis plate in order to allow the cells to be deposited on the depositing zone of the plate via decantation and a second position remote from this analysis plate in order to place the chamber in a fluid relationship with the absorption material in order to allow this material to absorb the fixing agent.

Abstract: A tray for carrying at least one bottle that has to be positioned and held precisely on the tray, includes a support on which the bottle is intended to be placed, at least two rails running substantially parallel to one another along the support, the rails including at least one shoulder extending above the support toward the other rail so that a lower edge of the bottle can fit between and be immobilized in the rails in a longitudinal or transverse direction and in an upwards direction, each rail having at least one imprint, the imprints being positioned facing one another, each imprint being designed to accommodate an immobilizing element provided on the bottle so that the bottle can be immobilized in the rails in a longitudinal or transverse direction. A bottle used with a tray such as this is also described.

Abstract: This method includes the following steps of: taking a sample of a cell solution from a sample flask, placing the sampled solution in a decantation chamber (2) arranged above the analysis plate (4), allowing the solution to decant in order to obtain a thin layer of cells on the analysis plate (4). It includes a step of measuring the cell density of the sampled solution, the measurement being carried out in the decantation chamber (2). A device for producing a cell analysis plate allowing such a method to be carried out is also described.

Abstract: The invention relates to a method that includes the following steps: carrying out a treatment of a sample, the treatment being carried out for differentiating diseased cells from healthy cells in the sample (4); carrying out at least one first image acquisition of the sample (4) provided on an analysis plate (8) so as to obtain a plurality of images, each representing an area (18) of the analysis plate, said images being arranged side by side so as to form an image of the entirety of the sample in order to create a virtual analysis plate (2), locating a reference plane of the analysis plate including a slide and a lamella provided above the slide for image acquisition, said reference plane being defined by the surface of the slide or the lamella; and carrying out at least one second image acquisition, said second acquisition being carried out at a different thickness of the sample relative to the first acquisition so as to obtain a plurality of images corresponding to a section of the sample with a differe

Abstract: A method includes carrying out at least a first processing of a sample (2), the processing being adapted to mark pathological cells among the sample cells; and carrying out several image acquisitions of the sample (2) provided on an analysis plate (6) in order to obtain images each representing an area of the analysis plate (6) wherein the images set side by side define an image (20) of the entire sample. The method further includes automatically running all the acquired images of the sample (2) on a display at a predetermined running speed, the speed being adapted for enabling an observer to analyze the image and to determine if the represented sample area includes potentially pathological cells or not; and stopping the running if at least one abnormality is detected that may indicate the presence of a pathological cell.

Abstract: A system for preparing a cytological suspension is based on a fixing solution. The system containing a cytological sample-taking brush and a flask having an opening for receiving the cytological sample-taking brush are detachably fastened to a handle including a brush-receiving filter basket that is at least partially immersed in the a suspension.

Abstract: This fixing solution is intended to preserve in vitro a cytological sample, comprising nucleated cells and erythrocytes and comprises alcohol for fixing the cells. It comprises physiological saline in order to avoid osmotic shocks at the wall of the cells, formalin and polyethylene glycol for retaining the size and the integrity of the nucleated cells and erythrocytes, which are fixed in said solution. The solution does not contain acetone or any compounds of the ketone or acetic acid family.

Abstract: The present invention relates to a process for preparing and analysing a plurality of cell suspensions (5) comprising at least the following successive steps: (a) loading a plurality of bottles (4) onto a reception plate (6), each bottle (4) comprising a cell suspension (5) to be analysed; (b) loading a plurality of analysis containers (32, 34, 36) onto the reception plate (6); and (c) taking a sample of a cell suspension (5) from a bottle (4) and depositing this sample in an analysis container (34, 36); wherein step (c) is repeated for each bottle (4) to be analysed. Step (c) of taking a sample of a cell suspension from a bottle (4) and depositing this sample in an analysis container (34, 36) comprises at least one step of breaking up the cell clusters by virtue of pipetting distribution means (20). The invention also relates to an automated device for implementing this process.

Abstract: A device includes a reception chamber (5) for a cell suspension having an open bottom (6) extending opposite a cell deposition area (7) of the analysis plate (1), the bottom (6) of the chamber (5) being in fluid communication with an absorption material (14) for cell preservation liquid for absorbing the latter and enabling a homogeneous decantation deposition on the deposition area (7), the absorption material (14) being provided about the cell deposition area (7). The absorption material (14) includes a compressed area (16) extending between the bottom (6) of the reception chamber (5) and the analysis plate (1) about the cell deposition area (7), and a non-compressed area (17) extending about the compressed area (16).

Abstract: A system for analyzing a cell suspension includes at least one well for decanting (1) the suspension and elements (2, 3) for analyzing the cell deposits at the bottom of the well over time during the decanting, so as to allow an analysis of the cells by strata (T1, T2, T3) as a function of their rate of decantation.

Abstract: A system for depositing cells on an analysis plate, the cells being contained in a cell suspension including a fixing agent and the cells, included, for receiving the suspension, a chamber (5) which is positioned above the analysis plate and whose base is open and extends opposite a cell depositing zone of the plate and a material for absorbing the fixing agent placed around the chamber and the depositing zone of the plate. The chamber is mounted so as to be able to be moved between a first position in abutment against the analysis plate in order to allow the cells to be deposited on the depositing zone of the plate via decantation and a second position remote from this analysis plate in order to place the chamber in a fluid relationship with the absorption material in order to allow this material to absorb the fixing agent.

Abstract: The invention relates to a method that includes the following steps: carrying out a treatment of a sample, the treatment being carried out for differentiating diseased cells from healthy cells in the sample (4); carrying out at least one first image acquisition of the sample (4) provided on an analysis plate (8) so as to obtain a plurality of images, each representing an area (18) of the analysis plate, said images being arranged side by side so as to form an image of the entirety of the sample in order to create a virtual analysis plate (2), locating a reference plane of the analysis plate including a slide and a lamella provided above the slide for image acquisition, said reference plane being defined by the surface of the slide or the lamella; and carrying out at least one second image acquisition, said second acquisition being carried out at a different thickness of the sample relative to the first acquisition so as to obtain a plurality of images corresponding to a section of the sample with a differe

Abstract: This method includes the following steps: carrying out a processing of the specimen so as to make it possible to differentiate the pathological cells from the healthy cells of the specimen; and performing an acquisition of images of the specimen disposed on an analysis plate so as to obtain a plurality of images each representing a zone of the analysis plate, the images placed side by side forming an image of the whole of the specimen so as to create a virtual analysis plate. The method furthermore includes the following step: performing on the virtual analysis plate a processing of the images acquired so as to obtain a virtual restitution of the colors and of the intensity of the colors of the cytoplasm and/or of the nucleus, the colors and the intensity being able to be modified according to the preferences of the person in charge of the analysis.

Abstract: A method includes carrying out at least a first processing of a sample (2), the processing being adapted to mark pathological cells among the sample cells; and carrying out several image acquisitions of the sample (2) provided on an analysis plate (6) in order to obtain images each representing an area of the analysis plate (6) wherein the images set side by side define an image (20) of the entire sample. The method further includes automatically running all the acquired images of the sample (2) on a display at a predetermined running speed, the speed being adapted for enabling an observer to analyze the image and to determine if the represented sample area includes potentially pathological cells or not; and stopping the running if at least one abnormality is detected that may indicate the presence of a pathological cell.

Abstract: This method includes the following steps of: taking a sample of a cell solution from a sample flask, placing the sampled solution in a decantation chamber (2) arranged above the analysis plate (4), allowing the solution to decant in order to obtain a thin layer of cells on the analysis plate (4). It includes a step of measuring the cell density of the sampled solution, the measurement being carried out in the decantation chamber (2). A device for producing a cell analysis plate allowing such a method to be carried out is also described.

Abstract: A device includes a reception chamber (5) for a cell suspension having an open bottom (6) extending opposite a cell deposition area (7) of the analysis plate (1), the bottom (6) of the chamber (5) being in fluid communication with an absorption material (14) for cell preservation liquid for absorbing the latter and enabling a homogeneous decantation deposition on the deposition area (7), the absorption material (14) being provided about the cell deposition area (7). The absorption material (14) includes a compressed area (16) extending between the bottom (6) of the reception chamber (5) and the analysis plate (1) about the cell deposition area (7), and a non-compressed area (17) extending about the compressed area (16).

Abstract: A system for analyzing a cell suspension includes at least one well for decanting (1) the suspension and elements (2, 3) for analyzing the cell deposits at the bottom of the well over time during the decanting, so as to allow an analysis of the cells by strata (T1, T2, T3) as a function of their rate of decantation.

Abstract: A tray for carrying at least one bottle that has to be positioned and held precisely on the tray, includes a support on which the bottle is intended to be placed, at least two rails running substantially parallel to one another along the support, the rails including at least one shoulder extending above the support toward the other rail so that a lower edge of the bottle can fit between and be immobilized in the rails in a longitudinal or transverse direction and in an upwards direction, each rail having at least one imprint, the imprints being positioned facing one another, each imprint being designed to accommodate an immobilizing element provided on the bottle so that the bottle can be immobilized in the rails in a longitudinal or transverse direction. A bottle used with a tray such as this is also described.