Abstract: An apparatus of conducting electrophoresis experiments including a container for receiving an electrophoresis buffer and a buffer core assembly for holding gel-containing cassettes that are immersed in the buffer for molecular separation of an electrophoresis sample. The buffer core assembly defines a flow path for the electrophoresis buffer which can be circulated by means of a heat exchanger and a pump to effect controlled heat exchange between the buffer and the cassette surfaces thereby maintaining the gel and the electrophoresis process at a desirable temperature uniformly over the surfaces of the gel cassette. The buffer core assembly also has upper well for receiving a second electrically chargeable buffer in contact with the gel so as to impose an electric field on the gel for the electrophoresis in conjunction with the first buffer. The well is isolated from the first buffer to prevent mixing of the buffers and to reduce the risk of an electrical short.
Type:
Grant
Filed:
March 28, 1997
Date of Patent:
March 30, 1999
Assignee:
Novel Experimental Technology
Inventors:
Barbara L. Tippins, Roumen Bogoev, Douglas R. Levy
Abstract: An improved plastic mold for the polymerization of electrophoresis gels. The improved electrophoresis gel mold comprises non-conductive plastic film. The film is supported by a rigid plastic frame. The frame maintains the integrity of the electrophoresis gel. The molds of the present invention are particularly useful in the manufacture of precast electrophoresis gels.
Type:
Grant
Filed:
September 16, 1996
Date of Patent:
May 19, 1998
Assignee:
Novel Experimental Technology
Inventors:
William F. Alpenfels, Sheldon Engelhorn, David Manis
Abstract: An improved coated plastic mold for the polymerization of electrophoresis gels. The mold controls the effects of oxygen contamination upon gel polymerization resulting in a more uniform gel with improved properties.
Abstract: A method and apparatus for drying electrophoresis gels. The apparatus includes a base unit and gel drying frame assembly. The base unit serves as a both a support for the frame when placing the gel in the frame and as a receptacle for excess liquid used to prepare the gel for the drying process. The frame is used to securely hold the gel in place while preparing the gel for drying and also during the drying process itself.
Type:
Grant
Filed:
August 4, 1995
Date of Patent:
November 12, 1996
Assignee:
Novel Experimental Technology
Inventors:
William F. Alpenfels, Sheldon Engelhorn, David J. Manis
Abstract: A method and apparatus for drying electrophoresis gels. The apparatus includes a base unit and gel drying frame assembly. The base unit serves as a both a support for the frame when placing the gel in the frame and as a receptacle for excess liquid used to prepare the gel for the drying process. The frame is used to securely hold the gel in place while preparing the gel for drying and also during the drying process itself.
Type:
Grant
Filed:
July 16, 1993
Date of Patent:
August 15, 1995
Assignee:
Novel Experimental Technology
Inventors:
William F. Alpenfels, Sheldon Engelhorn, David J. Manis