Abstract: A sample-to-answer microfluidic system and method including a microfluidic instrument with a detection system for detecting target assay products, data display, vertically oriented receiving member for receiving a microfluidic cartridge and an actuator assembly including a vertically oriented actuator. The system also includes a microfluidic cartridge with reagent pouches including a flow through reagent pouch, reagent wells in fluidic connection with one another. Methods of using the sample-to-answer microfluidic system are also provided.

Abstract: A sample-to-answer microfluidic system and method including vertical microfluidic device and automated actuation mechanisms, such as, but not limited to, automated mechanical and/or magnetic actuation, is disclosed. In some embodiments, the sample-to-answer microfluidic system includes a vertically oriented microfluidic device and a rotating actuator in relation to the microfluidic device, and wherein the microfluidic device and the rotating actuator are operating in the XZ plane. Additionally, methods of using the sample-to-answer microfluidic system are provided.

Abstract: Disclosed herein is an apparatus for controlling assay processes performed in a microfluidic cartridge used in sample-to-answer and point-of-care diagnostic instruments. The apparatus controls reagent dispensing into a microfluidic cartridge, magnetic bead based movement of analytes within a microfluidic cartridge, as well as nucleic acid sample preparation, amplification, and detection processes.

Abstract: The present invention provides methods and devices for simple, low power, automated processing of biological samples through multiple sample preparation and assay steps. The methods and devices described facilitate the point-of-care implementation of complex diagnostic assays in equipment-free, non-laboratory settings.

Abstract: Disclosed herein is an apparatus for containing and dispensing reagent into a microfluidic cartridge for use in sample-to-answer or point-of-care instruments. The apparatus contains a reagent dispensing unit, such as a reagent blister. The blister can contain one or more vessels for containment of reagents and a rupturable seal that blocks the flow of reagent from the reagent blister through an interface and into the microfluidic cartridge.

Abstract: The presently disclosed subject matter provides devices and methods for sample extraction from a swab during biological sample processing. In particular embodiments, the devices and methods are configured for use in conjunction with microfluidic devices for sample processing.

Abstract: The presently disclosed subject matter provides devices and methods for sample extraction from a swab during biological sample processing. In particular embodiments, the devices and methods are configured for use in conjunction with microfluidic devices for sample processing.

Abstract: The present invention provides methods and devices for simple, low power, automated processing of biological samples through multiple sample preparation and assay steps. The methods and devices described facilitate the point-of-care implementation of complex diagnostic assays in equipment-free, non-laboratory settings.

Abstract: An apparatus and method for simple, low power, automated processing of biological samples through multiple preparation and assay steps. The apparatus and methods described facilitate the point-of-care implementation of diagnostic assays. The apparatus includes mechanical actuating elements using linear and/or rotational motion.

Abstract: The present invention provides methods and devices for simple, low power, automated processing of biological samples through multiple sample preparation and assay steps. The methods and devices described facilitate the point-of-care implementation of complex diagnostic assays in equipment-free, non-laboratory settings.