Abstract: A device for the electrochemical detection of at least one biochemical molecule contained in a liquid from a group of predetermined biochemical molecules includes a holder (1) for taking up the liquid, with at least one reference electrode (RE) and at least one counterelectrode (GE) and a multiplicity of working electrodes (AE1, AE2, AE3). At least one working electrode is provided for the detection of each biochemical molecule, and is coated with a molecule that is complementary to the respective biochemical molecule, so that the biochemical molecules can be detected simultaneously. The device includes a potentiostat (P) for generating a predetermined voltage profile between the working electrodes and the reference electrode, a current/voltage converter (S1, S2, S3) connected downstream of each of the working electrodes for holding all of the working electrodes at the same potential, and a measurement device (Ad) for measuring the currents flowing through the working electrodes.
Type:
Grant
Filed:
February 10, 2005
Date of Patent:
January 30, 2007
Assignee:
November Aktiengesellschaft Gesellschaft für Molekulare Medizin
Inventors:
Jürgen Schülein, Björn Grassl, Jörg Hassmann
Abstract: The invention relates to a method for identifying a first polymer (4, 7) which is bonded to a first phase (5) that reflects electromagnetic waves. Said method comprises the following steps: (a) bringing the first polymer (4, 7) into contact with a second polymer (3, 8), which is bonded to a solid second phase (1) by metallic clusters (2), said second phase being permeable to electromagnetic waves; (b) irradiating the second phase (1) with electromagnetic waves; and (c) determining the change in the properties of the reflected electromagnetic waves.
Type:
Grant
Filed:
June 10, 2000
Date of Patent:
June 27, 2006
Assignee:
november Aktiengesellschaft Gesellschaft für Molekulare Medizin
Abstract: A method is disclosed for detecting a nucleotide sequence by a polymerase chain reaction, the nucleotide sequence is a constituent of a double-stranded nucleic acid molecule which is in solution and formed of a strand and a counter-strand. A first primer is added to the solution and is complementary to a first segment of the strand located at the 5′-terminus of a central segment. A second primer is added and is complementary to a 5′-terminal second segment of the counter-strand. The solution is brought into contact with a third primer whose 5′-terminal end is bound to a solid phase. The third primer is complementary to the central segment of the strand containing the nucleotide sequence. A reaction vessel is closed which contains the solution. The solution is repeatedly heated and cooled and the nucleic acid molecule is detected in the closed reaction vessel.
Type:
Grant
Filed:
November 16, 2000
Date of Patent:
June 11, 2002
Assignee:
November Aktiengesellschaft Gesellschaft fuer Molekulare
Medizin