Abstract: A sample buffer and method are provided for preparing denatured nucleic acids and applying them to gel electrophoresis systems. The sample buffer and method enhance band flatness and sharpness, resulting in higher resolution and throughput.
Type:
Grant
Filed:
January 23, 1998
Date of Patent:
May 2, 2000
Assignee:
Novex
Inventors:
Timothy V. Updyke, Roumen A. Bogoev, Song-Hua Ke
Abstract: An apparatus for clamping electrophoresis gel cassettes to a buffer core body is provided that does not require separate clamping subassemblies. The apparatus comprises a cam pivotally coupled to a mounting block. The apparatus is inserted into an electrophoresis container and is positioned adjacent a buffer core assembly that includes gel cassettes disposed on opposite sides of a buffer core body. The cam is disposed to engage a back wall of the container to cause the mounting block to apply uniform pressure to secure the gel cassettes to the buffer core body. A method for securing an electrophoresis gel cassette to a buffer core body in an electrophoresis container is also disclosed.