Abstract: Expanded bed isomerization of glucose syrup to a glucose-fructose mixture having a fructose proportion exceeding about 40% of the glucose-fructose content by passing 30-55% w/w of glucose syrup through a cell-mass glucose isomerase preparation in the form of a bed of uniform particles of controlled size in the range of 150-2000 micrometers at a superficial syrup velocity of 0.5-15 meters an hour. Velocity and particle size are inversely related to create bed expansion within the range of 5-100%.Bed life may be extended by substituting fresh catalyst for inactivated catalyst.The isomerase should have an activity exceeding about 100 IGIC u/gm.The isomerase should be weighted by incorporation therein of finely divided alumina particles.

Abstract: An enzymatic intralenticular cataract treatment method for removal of nuclear cortical and subcapsular regions of the cataractous lens through enzymatic digestion thereof, which comprises introduction of a concentrated solution of proteinase into the nuclear and cortical regions of a cataractous lens by way of a cannula inserted through a very tiny incision in the cornea and puncture opening into the lens capsule, leaving all other structures within the eye intact, after which a gas bubble is introduced from the cannula into the lens, the gas bubble serving to maintain the enzyme compartmentalized within the subcapsular region.As illustrated in FIG. 4, a preferred embodiment of the apparatus involved with the method comprises an enzyme reservoir connected through a delivery means and a four port, two channel valve to a cannula. The cannula is filled with enzyme solution then the delivery means and valve are operated by the surgeon so as to carry out the above described treatment method.