Patents Assigned to Novo Nordisk Biotech, Inc.
  • Metalloprotease having increased activity
    Patent number: 5807729
    Abstract: The present invention relates to a novel metalloprotease obtainable from a fungus having increased proteolytic activity. Additionally, the invention related to isolated nucleic acid fragments encoding said metalloprotease as well as vectors, DNA constructs, and recombinant host cells comprising said nucleic acid fragments.
    Type: Grant
    Filed: May 17, 1995
    Date of Patent: September 15, 1998
    Assignees: Novo Nordisk A/S, Novo Nordisk Biotech, Inc.
    Inventors: Jeffrey R. Shuster, Mark Madden, Donna L. Moyer, Claus Fuglsang, Sven Branner
  • Purified Myceliophthora laccases and nucleic acids encoding same
    Patent number: 5795760
    Abstract: The present invention relates to isolated nucleic acid constructs containing a sequence encoding a Myceliophthora laccase, and the laccase proteins encoded thereby.
    Type: Grant
    Filed: September 29, 1997
    Date of Patent: August 18, 1998
    Assignees: Novo Nordisk A/S, Novo Nordisk Biotech, Inc.
    Inventors: Randy Michael Berka, Stephen H. Brown, Feng Xu, Palle Schneider, Karen M. Oxenb.o slashed.ll, Dorrit A. Aaslyng
  • Mutants of Myceliophthora laccase with enhanced activity
    Patent number: 5770419
    Abstract: The present invention relates to mutants of a blue multi-copper oxidase, comprising (a) a substitution of one or more amino acid residues with other amino acid residues, (b) an insertion of one or more amino acid residues and/or (c) a deletion of one or more amino acid residues, wherein the substitution, insertion or deletion is carried out at a position which is located no greater than 15 .ANG. from a Type I (T1) copper site. The present invention also relates to nucleic acid constructs comprising a nucleic acid sequence encoding the mutants of the present invention, host cells comprising the construct of the present invention, and methods for producing mutants of the present invention.
    Type: Grant
    Filed: August 30, 1996
    Date of Patent: June 23, 1998
    Assignee: Novo Nordisk Biotech, Inc.
    Inventors: Xu Feng, Randy M. Berka, Jill Angela Wahleithner
  • Modification of cryptic splice sites in heterologous genes expressed in fungi
    Patent number: 5770371
    Abstract: The present invention relates to methods for obtaining a fungal host cell comprising a nucleic acid sequence encoding a heterologous polypeptide, wherein at least one cryptic splice site is modified in the nucleic acid sequence. The present invention also relates to a nucleic acid sequence(s) with a modified cryptic splice site(s) as well as nucleic acid constructs, vectors, and host cells comprising said nucleic acid sequence(s). The present invention further relates to methods for recombinant production of a polypeptide encoded by said nucleic acid sequence.
    Type: Grant
    Filed: June 27, 1996
    Date of Patent: June 23, 1998
    Assignee: Novo Nordisk Biotech, Inc.
    Inventor: Sheryl Thompson
  • Purified polyporus laccases and nucleic acids encoding same
    Patent number: 5770418
    Abstract: The present invention relates to isolated nucleic acid constructs containing a sequence encoding a Polyporus laccase, and the laccase proteins encoded thereby.
    Type: Grant
    Filed: May 15, 1995
    Date of Patent: June 23, 1998
    Assignees: Novo Nordisk A/S, Novo Nordisk Biotech. Inc.
    Inventors: Debbie Sue Yaver, Feng Xu, Henrik Dalb.o slashed.ge, Palle Schneider, Dorrit A. A.ae butted.lyng
  • Method of producing iodine by use of a copper containing oxidase enzyme
    Patent number: 5766896
    Abstract: The present invention relates to a method for oxidation of iodide which comprises contacting, in an aqueous solution, a copper-containing oxidaze enzyme and a source of ionic iodide(I.sup.-), for a time and under conditions sufficient to permit the conversion of ionic iodide to iodine by the enzyme. The copper-containing enzymes may be, for example, a laccase or a bilirubin oxidaze.
    Type: Grant
    Filed: November 22, 1994
    Date of Patent: June 16, 1998
    Assignee: Novo Nordisk Biotech Inc.
    Inventor: Feng Xu
  • Bacterial donor cell useful in conjugation
    Patent number: 5763187
    Abstract: The present invention relates to a modified bacterial donor cell useful in conjugation, the cell containing i) a plasmid comprising a DNA construct encoding the polypeptide of interest, and at least one cis-acting DNA sequence required for the transfer of said plasmid by conjugation in the presence of a trans-acting mobilizing element, and ii) at least one DNA sequence encoding said trans-acting mobilizing element, wherein the donor cell has a reduced capacity, relative to a parent cell, of producing a bactericidal agent which kills or prevents growth in a population of recipient cells.
    Type: Grant
    Filed: January 17, 1997
    Date of Patent: June 9, 1998
    Assignee: Novo Nordisk Biotech, Inc.
    Inventors: Alan P. Sloma, William R. Widner
  • Method for solubilizing proteins in organic solvents
    Patent number: 5756331
    Abstract: The present invention relates to a method for producing a protein composition soluble in organic solvents, comprising mixing a protein of interest with a surfactant and a water immiscible organic solvent in amounts and under conditions conducive to the formation of a reverse micelle solution, and evaporating the resulting reverse micelle solution to dryness.
    Type: Grant
    Filed: December 3, 1996
    Date of Patent: May 26, 1998
    Assignee: Novo Nordisk Biotech, Inc.
    Inventor: Alexander Blinkovsky
  • Gene encoding carboxypeptidase of Aspergillus niger
    Patent number: 5705376
    Abstract: The present invention relates to a gene encoding an ascomycete or deuteromycete carboxypeptidase Y gene, and host cells modified so as to produce reduced amounts of carboxypeptidase.
    Type: Grant
    Filed: February 28, 1996
    Date of Patent: January 6, 1998
    Assignee: Novo Nordisk Biotech, Inc.
    Inventors: Debbie Sue Yaver, Sheryl Ann Thompson
  • Processes for producing an enzyme
    Patent number: 5702934
    Abstract: The present invention is related to a process for producing an active enzyme comprising fermenting the proform of the active enzyme in the present of a proteolytic enzyme different from the active enzyme and capable of converting the proenzyme into an active enzyme as well as to host cells, recombinant expression vectors and host cells suitable for use in the process.
    Type: Grant
    Filed: May 4, 1994
    Date of Patent: December 30, 1997
    Assignees: Novo Nordisk A/S, Novo Nordisk Biotech, Inc.
    Inventors: Sven Hastrup, Sven Branner, Birthe Ravn J.o slashed.rgensen, Tove Christensen, Birgitte Bojer J.o slashed.rgensen, Jeffrey R. Shuster, Mark Madden, Donna L. Moyer, Claus Fuglsang
  • Thermophilic fungal expression system
    Patent number: 5695985
    Abstract: The present invention relates to recombinant thermophilic host cells comprising a nucleic acid sequence encoding a heterologous protein, and a method of producing recombinant protein utilizing same. The recombinant hosts of the present invention provide a better morphology in tank fermentations than many known fungal host cells, such as Aspergillus, which morphology results in lower viscosity levels, and therefore improved productivity.
    Type: Grant
    Filed: June 1, 1995
    Date of Patent: December 9, 1997
    Assignee: Novo Nordisk Biotech, Inc.
    Inventors: Ejner Bech Jensen, Karuppan Chettiar Boominathan
  • Method for solubilizing proteins in organic solvents
    Patent number: 5693516
    Abstract: The present invention relates to a method for producing a protein composition soluble in organic solvents, comprising mixing a protein of interest with a surfactant and a water immiscible organic solvent in amounts and under conditions conducive to the formation of a reverse micelle solution, and evaporating the resulting reverse micelle solution to dryness.
    Type: Grant
    Filed: November 27, 1995
    Date of Patent: December 2, 1997
    Assignee: Novo Nordisk Biotech, Inc.
    Inventor: Alexander Blinkovsky
  • Gene encoding carboxypeptidase of Aspergillus niger
    Patent number: 5693510
    Abstract: The present invention relates to a gene encoding an ascomycete or deuteromycete carboxypeptidase Y gene, and host cells modified so as to produce reduced amounts of carboxypeptidase.
    Type: Grant
    Filed: February 28, 1996
    Date of Patent: December 2, 1997
    Assignee: Novo Nordisk Biotech, Inc.
    Inventors: Debbie Sue Yaver, Sheryl Ann Thompson
  • Metalloprotease having increased activity
    Patent number: 5691162
    Abstract: The present invention relates to a novel metalloprotease obtainable from a fungus having increased proteolytic activity. Additionally, the invention related to isolated nucleic acid fragments encoding said metalloprotease as well as vectors, DNA constructs, and recombinant host cells comprising said nucleic acid fragments.
    Type: Grant
    Filed: May 17, 1995
    Date of Patent: November 25, 1997
    Assignees: Novo Nordisk A/S, Novo Nordisk Biotech, Inc.
    Inventors: Jeffrey R. Shuster, Mark Madden, Donna L. Moyer, Claus Fuglsang, Sven Branner
  • Gene encoding carboxypeptidase of Aspergillus niger
    Patent number: 5688663
    Abstract: The present invention relates to a gene encoding an ascomycete or deuteromycete carboxypeptidase Y gene, and host cells modified so as to produce reduced amounts of carboxypeptidase.
    Type: Grant
    Filed: February 28, 1996
    Date of Patent: November 18, 1997
    Assignee: Novo Nordisk Biotech, Inc.
    Inventors: Debbie Sue Yaver, Sheryl Ann Thompson
  • Aspergillus expression system
    Patent number: 5667990
    Abstract: The present invention relates to a novel expression system in which A. japonicus-type species are used as host cells for expression of heterologous proteins.
    Type: Grant
    Filed: June 1, 1995
    Date of Patent: September 16, 1997
    Assignee: Novo Nordisk Biotech, Inc.
    Inventors: Randy Michael Berka, Wendy Yoder, Shinobu Takagi, Karuppan Chettier Boominathan
  • Dye compositions containing purified polyporus laccases and nucleic acids encoding same
    Patent number: 5667531
    Abstract: The present invention relates to isolated nucleic acid constructs containing a sequence encoding a polyporus laccase, and the laccase proteins encoded thereby.
    Type: Grant
    Filed: June 5, 1995
    Date of Patent: September 16, 1997
    Assignees: Novo Nordisk A/S, Novo Nordisk Biotech, Inc.
    Inventors: Debbie Sue Yaver, Feng Xu, Henrik Dalb.o slashed.ge, Palle Schneider, Dorrit A. Aaslyng
  • Promoter element and signal peptide of a gene encoding a Bacillus alkaline protease and vectors comprising same
    Patent number: 5650326
    Abstract: The invention is directed to an isolated nucleic acid construct(s) comprising a nucleic acid sequence encoding an alkaline protease having a molecular weight of about 34 kD and is obtainable from a strain of Bacillus sp. Group I, as well as recombinant vectors and host cells comprising such constructs. Additionally, the invention is directed to a method for obtaining the protease. The invention is also directed to promoter and signal sequences derived from said alkaline protease as well as methods of using the promoter and signal sequences in the expression of heterologous nucleic acid sequences encoding heterologous proteins in bacteria.
    Type: Grant
    Filed: June 2, 1995
    Date of Patent: July 22, 1997
    Assignees: Novo Nordisk Biotech, Inc., Novo Nordisk A/S
    Inventors: Alan P. Sloma, Helle Outtrup, Claus Dambmann, Dorrit Anita Aaslyng
  • Recombinant methods for the production of a bacillus alkaline protease
    Patent number: 5622850
    Abstract: The invention is directed to an isolated nucleic acid construct(s) comprising a nucleic acid sequence encoding an alkaline protease having a molecular weight of about 34 kD and is obtainable from a strain of Bacillus sp. Group I, as well as recombinant vectors and host cells comprising such constructs. Additionally, the invention is directed to a method for obtaining the protease. The invention is also directed to promoter and signal sequences derived from said alkaline protease as well as methods of using the promoter and signal sequences in the expression of heterologous nucleic acid sequences encoding heterologous proteins in bacteria.
    Type: Grant
    Filed: June 2, 1995
    Date of Patent: April 22, 1997
    Assignees: Novo Nordisk Biotech, Inc., Novo Nordisk A/S
    Inventors: Alan P. Sloma, Helle Outtrup, Claus Dambmann, Dorrit A. Aaslyng
  • Method for the production of heterologous polypeptides using a promoter element and signal peptide of a bacillus gene encoding an alkaline protease
    Patent number: 5622841
    Abstract: The invention is directed to an isolated nucleic acid construct(s) comprising a nucleic acid sequence encoding an alkaline protease having a molecular weight of about 34 kD and is obtainable from a strain of Bacillus sp. Group I, as well as recombinant vectors and host cells comprising such constructs. Additionally, the invention is directed to a method for obtaining the protease. The invention is also directed to promoter and signal sequences derived from said alkaline protease as well as methods of using the promoter and signal sequences in the expression of heterologous nucleic acid sequences encoding heterologous proteins in bacteria.
    Type: Grant
    Filed: June 2, 1995
    Date of Patent: April 22, 1997
    Assignees: Novo Nordisk Biotech, Inc., Novo Nordisk A/S
    Inventors: Alan P. Sloma, Helle Outtrup, Claus Dambmann, Dorrit A. Aaslyng