Abstract: Disclosed are transformed cells comprising one or more genetic modifications that increase the lipid content of the cell, e.g., relative to an unmodified cell of the same type. Also disclosed are methods for increasing the lipid content of a cell by increasing the activity of one or more proteins in the cell and/or by decreasing the activity of one or more proteins in the cell.
Type:
Grant
Filed:
December 29, 2015
Date of Patent:
September 1, 2020
Assignee:
NOVOGY, INC.
Inventors:
Arthur J. Shaw, IV, Johannes Pieter Van Dijken, Annapurna Kamineni, Jonathan Friedlander, Vasiliki Tsakraklides, Maureen Hamilton, Elena E. Brevnova
Abstract: Disclosed are nucleotide sequences and corresponding amino acid sequences of Arxula adeninivorans genes that can be utilized to manipulate the lipid content and/or composition of a cell. Methods and compositions for utilizing this information are disclosed to increase the lipid content or modify the lipid composition of a cell by either increasing or decreasing the activity of certain genetic targets.
Type:
Grant
Filed:
May 29, 2015
Date of Patent:
July 28, 2020
Assignee:
NOVOGY, INC.
Inventors:
Elena E. Brevnova, Arthur J. Shaw, IV, Johannes P. Van Dijken
Abstract: DGA1 catalyzes the final enzymatic step for converting acyl-CoA and 1,2-diacylglycerol to triacylglycerols (TAG) and CoA in yeast. Disclosed are methods for expression in an oleaginous yeast host of polynucleotide sequences encoding DGA1 from Rhodosporidium toruloides, Lipomyces starkeyi, Aurantiochytrium limacinium, Aspergillus terreus, or Claviceps purpurea. Also described herein are engineered recombinant host cells of Yarrowia lipolytica comprising heterologous DGA1 polynucleotides encoding DGA1 proteins, or functionally active portions thereof, having the capability of producing increased lipid production and possessing the characteristic of enhanced glucose consumption efficiency.
Type:
Grant
Filed:
February 24, 2015
Date of Patent:
April 16, 2019
Assignee:
NOVOGY, INC.
Inventors:
Elena E. Brevnova, Arthur J. Shaw, IV, Emily H. Greenhagen
Abstract: DGA1 catalyzes the final enzymatic step for converting acyl-CoA and 1,2-diacylglycerol to triacylglycerols (TAG) and CoA in yeast. Disclosed are methods for expression in an oleaginous yeast host of polynucleotide sequences encoding DGA1 from Rhodosporidium toruloides, Lipomyces starkeyi, Aurantiochytrium limacinium, Aspergillus terreus, or Claviceps purpurea. Also described herein are engineered recombinant host cells of Yarrowia lipolytica comprising heterologous DGA1 polynucleotides encoding DGA1 proteins, or functionally active portions thereof, having the capability of producing increased lipid production and possessing the characteristic of enhanced glucose consumption efficiency.
Type:
Application
Filed:
February 24, 2015
Publication date:
December 15, 2016
Applicant:
NOVOGY, INC.
Inventors:
Elena E. Brevnova, Arthur J. Shaw, Emily H. Greenhagen
Abstract: Disclosed are genetically engineered organisms, such as yeast and bacteria, that have the ability to metabolize atypical nitrogen sources, such as melamine and cyanamide. Fermentation methods using the genetically engineered organisms are also described. The methods of the invention are robust processes for the industrial bioproduction of a variety of compounds, including commodities, fine chemicals, and pharmaceuticals.