Patents Assigned to Novozymes Delta, Limited
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Publication number: 20110020865Abstract: The present invention provides a host cell suitable for enhanced production of a protein product of choice characterised in that the host cell is genetically modified to cause over-expression of two or more helper proteins selected from a DnaJ-like protein (such as JEM1), an Hsp70 family protein (such as LHS1) and SIL1 wherein at least one of the over-expressed two or more helper proteins is selected from JEM1, LHS1 and SIL1, and wherein the DnaJ-like protein is not SCJ1.Type: ApplicationFiled: June 22, 2006Publication date: January 27, 2011Applicants: Novozymes Delta Limited, University of NottighamInventors: Thomas Payne, Darrell Sleep, Christopher John Arthur Finnis, Leslie Robert Evans
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Publication number: 20080274969Abstract: The invention relates to proteins comprising serine protease inhibiting peptides, such as Kunitz domain peptides (including, but not limited to, fragments and variants thereof) fused to albumin, or fragments or variants thereof. These fusion proteins are herein collectively referred to as “albumin fusion proteins of the invention.” These fusion proteins exhibit extended shelf-life and/or extended or therapeutic activity in solution. The invention encompasses therapeutic albumin fusion proteins, compositions, pharmaceutical compositions, formulations and kits. The invention also encompasses nucleic acid molecules and vectors encoding the albumin fusion proteins of the invention, host cells transformed with these nucleic acids and vectors, and methods of making the albumin fusion proteins of the invention using these nucleic acids, vectors, and/or host cells. The invention also relates to compositions and methods for inhibiting neutrophil elastase, kallikrein, and plasmin.Type: ApplicationFiled: May 2, 2008Publication date: November 6, 2008Applicants: NOVOZYMES DELTA LIMITED, DYAX CORP.Inventors: Hans-Peter Hauser, Thomas Weimer, Val Romberg, Scott M. Kee, Darrell Sleep, Robert Charles Ladner, Arthur C. Ley
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Publication number: 20080193977Abstract: The present invention provides a method for producing a desired protein (such as a desired heterologous protein) comprising: (a) providing a host cell comprising a first recombinant gene encoding a protein comprising the sequence of a first chaperone protein, a second recombinant gene encoding a protein comprising the sequence of a second chaperone protein and a third gene, such as a third recombinant gene, encoding a desired protein (such as a desired heterologous protein), wherein the first and second chaperones are different; and (b) culturing the host cell in a culture medium to obtain expression of the first, second and third genes.Type: ApplicationFiled: December 23, 2005Publication date: August 14, 2008Applicant: Novozymes Delta LimitedInventors: Christopher John Arthur Finnis, Darrell Sleep, Gillian Shuttleworth
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Publication number: 20080009042Abstract: A process is provided for the preparation of albumin which has extremely low levels of or is essentially free of colorants, metal ions, human proteins, host proteins, fragments of albumin, polymers or aggregates of albumin and viruses, and which is essentially non-glycated, relatively high in free thiol and with an intact C-terminus. The process comprises passing albumin (preferably expressed and secreted by transformed yeast) through positive mode cation exchange and then positive mode anion exchange chromatography. Other steps may also be employed, for example ultrafiltration, gel permeation chromatography, affinity chromatography binding the albumin and affinity chromatography binding contaminants. Elution of albumin, with a compound having affinity for albumin, from a material having no specific affinity for albumin is also disclosed, as is removal of ammonium ions with a counter-ion.Type: ApplicationFiled: April 23, 2007Publication date: January 10, 2008Applicant: NOVOZYMES DELTA LIMITEDInventors: Andrew Goodey, Darell Sleep, Hendrik Van Urk, Stephen Berezenko, John Woodrow, Richard Johnson, Patricia Wood, Stephen Burton, Alan Quirk
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Patent number: 7223561Abstract: A process is provided for the preparation of albumin which has extremely low levels of or is essentially free of colorants, metal ions, human proteins, host proteins, fragments of albumin, polymers or aggregates of albumin and viruses, and which is essentially non-glycated, relatively high in free thiol and with an intact C-terminus. The process comprises passing albumin (preferably expressed and secreted by transformed yeast) through positive mode cation exchange and then positive mode anion exchange chromatography. Other steps may also be employed, ultrafiltration, gel permeation chromatography, affinity chromatography binding the albumin by using blue dyes) and affinity chromatography binding contaminants by using an aminophenylboronic acid resin. Elution of albumin, with a compound having affinity for albumin, from a material having no specific affinity for albumin is also disclosed, as is removal of ammonium ions with a counter-ion.Type: GrantFiled: June 22, 2004Date of Patent: May 29, 2007Assignee: Novozymes Delta, LimitedInventors: Andrew Robert Goodey, Darell Sleep, Hendrik Van Urk, Stephen Berezenko, John Rodney Woodrow, Richard Alan Johnson, Patricia Carol Wood, Stephen James Burton, Alan Victor Quirk