Abstract: The present invention provides an improved process for producing a fermentation product in a fermentation medium, including ethanol, which process include a fermentation step, comprising subjecting the fermentation medium to at least one surfactant and at least one carbohydrate-source generating enzyme.
Type:
Application
Filed:
January 14, 2005
Publication date:
September 18, 2008
Applicants:
Novozymes A/S, Novozymes North America, Inc.
Inventors:
Eric Allain, Kevin S. Wenger, Henrik Bisgard-Frantzen, Lori Henderson, Carmen Costable
Abstract: A novel fungal enzyme having lysozyme activity has been isolated. The invention further relates to a fungal polypeptide having lysozyme activity and belonging to the GH25 family, wherein the enzyme is selected from the group consisting of (a) a polypeptide comprising an amino acid sequence, which has at least 80% identity with amino acids 1 to 233 of SEQ ID NO:2; (b) a polypeptide comprising an amino acid sequence, which has at least 80% identity with the polypeptide encoded by the lysozyme encoding part of the nucleotide sequence inserted into a plasmid present in strain DSM 16084; (c) a polypeptide which is encoded by a nucleotide sequence which hybridizes under high stringency conditions with a polynucleotide probe consisting of the complementary strand of nucleotides 84 to 782 of SEQ ID NO:1; or (d) a fragment of (a), (b) or (c) that has lysozyme activity.
Abstract: A method of solubilizing protease crystals and/or protease precipitate in a fermentation broth comprising a) diluting the fermentation broth 100-2000% (w/w); b) adding a divalent salt; and c) adjusting the pH value of the fermentation broth to a pH value below pH 5.5.
Type:
Application
Filed:
March 7, 2008
Publication date:
September 18, 2008
Applicant:
Novozymes A/S
Inventors:
Sune Jakobsen, Kim Uhre Hansen, Svend Kaasgaard
Abstract: The present invention relates to granules comprising a core matrix and one or more coatings, wherein the core matrix comprises: An active compound, a synthetic polymer, where the amount of polymer added is 0.1 to 10% by weight of the matrix and one or more antioxidants and/or reducing agents, where the amount of antioxidant and/or reducing agent is 0.2 to 5% by weight of the matrix.
Type:
Grant
Filed:
June 30, 2003
Date of Patent:
September 16, 2008
Assignee:
Novozymes A/S
Inventors:
Ole Simonsen, Erik Kjaer Markussen, Hanne Rojel, Svend Kaasgaard, Thomas Honger Callisen, Christian Isak Jorgensen
Abstract: The present invention relates to phytase variants, their preparation and uses, which phytase variants, when aligned according to FIG. 1, are amended as compared to a model phytase in at least one of a number of positions. Preferred model phytases are basidiomycete and ascomycete phytases, such as Peniophora phytase and Aspergillus phytases. Preferred phytase variants exhibits amended activity characteristics, such as improved specific activity and/or improved thermostability.
Type:
Application
Filed:
November 19, 2007
Publication date:
September 11, 2008
Applicant:
Novozymes A/S
Inventors:
Allan Svendsen, Soren Flensted Lassen, Dirk Kostrewa, Luis Pasamontes, Martin Lehman, Andrea Tomschy, Adolphus Van Loon, Kurt Vogel, Markus Wyss
Abstract: New detergent compositions and the use of enzyme combinations therein are disclosed. The compositions have enhanced stability of non protease enzymes present in the compositions.
Type:
Application
Filed:
October 8, 2007
Publication date:
September 11, 2008
Applicant:
Novozymes A/S
Inventors:
Mikael Mikkelsen, Niels Munk Ryom, Claus Ladefoged, Sandra Friis-Jensen
Abstract: The present invention relates to phytase variants, their preparation and uses, which phytase variants, when aligned according to FIG. 1, are amended as compared to a model phytase in at least one of a number of positions. Preferred model phytases are basidiomycete and ascomycete phytases, such as Peniophora phytase and Aspergillus phytases. Preferred phytase variants exhibits amended activity characteristics, such as improved specific activity and/or improved thermostability.
Type:
Application
Filed:
November 19, 2007
Publication date:
September 11, 2008
Applicant:
Novozymes A/S
Inventors:
Allan Svendsen, Soren Flensted Lassen, Dirk Kostrewa, Luis Pasamontes, Martin Lehmann, Andrea Tomschy, Adolphus Van Loon, Kurt Vogel, Markus Wyss
Abstract: The present invention relates to polypeptides having phytase activity. These polypeptides have an amino acid sequence which has at least 70% identity to either of three phytases derived from the bacterium Buttiauxella, and which comprises at least one of the following amino acids at the position indicated: 119N, 120L, and/or 121E. These phytases have an improved specific activity. Additional specific amino acid substitutions are also disclosed which characterize and distinguish additional phytases of the invention having improved properties such as temperature and/or pH stability, pH activity profile, temperature activity profile, substrate profile, improved performance in animal feed in vitro or in vivo. The invention also relates to isolated polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
Type:
Application
Filed:
January 30, 2008
Publication date:
September 11, 2008
Applicant:
NOVOZYMES A/S
Inventors:
Carsten Sjoeholm, Soeren Flensted Lassen, Lars Kobberoee Skov, Leonardo De Maria
Abstract: The present invention relates to a method for producing cheese comprising treating cheese milk, or a fraction of cheese milk, with a phospholipase and adding whey protein to the cheese milk.
Type:
Application
Filed:
October 13, 2006
Publication date:
September 11, 2008
Applicants:
Novozymes A/S, Christian Hansen A/S
Inventors:
Tine Muxoll Fatum, Erik Hoeier, John Lyne, Mikkel Laust Broe
Abstract: An isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequence which has at least 70% identity with amino acids 22 to 450 of SEQ ID NO:4; b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334; c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO:3; and d) a fragment of (a), (b) or (c) that has alpha-amylase activity.
Type:
Application
Filed:
February 25, 2008
Publication date:
September 11, 2008
Applicant:
Novozymes A/S
Inventors:
Lan Tang, Wenping Wu, Junxin Duan, Pia-Francke Johannesen
Abstract: The present invention relates to an improved process of distilling fermented mash, wherein one or more amylases and/or proteases are added to the fermentation mash before or during distillation.
Type:
Application
Filed:
April 6, 2005
Publication date:
September 4, 2008
Applicants:
Novozymes North America, Inc., Broin and Associates, Inc.
Inventors:
Kevin S. Wenger, Eric Allain, Stephen M. Lewis, John Michael Finck, Debbie Lynn Roth
Abstract: The present invention relates to isolated polypeptides having antimicrobial activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
Abstract: The present invention relates to polypeptides having cellobiohydrolase II activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.
Type:
Application
Filed:
December 18, 2007
Publication date:
September 4, 2008
Applicant:
Novozymes A/S
Inventors:
Wenping Wu, Lene Lange, Dominique Aubert Skovlund, Ye Liu
Abstract: The present invention relates to polypeptides having alpha-amylase activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to a polypeptide having carbohydrate-binding affinity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.
Type:
Application
Filed:
December 5, 2007
Publication date:
September 4, 2008
Applicants:
Novozymes A/S, Novozymes North America, Inc.
Inventors:
Tine Hoff, Carsten Andersen, Tina Spendler, Sven Pedersen, Anders Vikso-Nielsen, Thomas Schafer, Jiyin Liu
Abstract: The invention relates to a variant of a parent fungal glucoamylase, which exhibits altered properties, in particular improved thermal stability and/or increased specific activity.
Type:
Application
Filed:
February 21, 2008
Publication date:
September 4, 2008
Applicant:
NOVOZYMES A/S
Inventors:
Bjarne Ronfeldt Nielsen, Allan Svendsen, Henrik Pedersen, Jesper Vind, Hanne Vang Hendriksen, Torben Peter Frandsen
Abstract: The present invention relates to polypeptides having antimicrobial activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.
Type:
Application
Filed:
June 10, 2004
Publication date:
September 4, 2008
Applicants:
Novozymes A/S, Universita' Degli Studi Di Trieste Dipartimento di Biochimica
Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes involved in the production of a trichothecene and (ii) the mutant produces less of the trichothecene than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells. The present invention also relates to isolated trichodiene synthases and isolated nucleic acid sequences encoding the trichodiene synthases.
Type:
Application
Filed:
January 31, 2008
Publication date:
September 4, 2008
Applicant:
Novozymes, Inc.
Inventors:
John C. Royer, Lynne M. Christianson, Gregory A. Gambetta, Howard Brody, Suzanne M. Otani, Wendy T. Yoder
Abstract: The present invention relates to a granule comprising a core and a coating, wherein the core comprises an active compound, and the coating comprises filaments prepared from atomizing a liquid coating composition having the property that liquid coating composition forms filaments upon atomization.
Abstract: The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 ?; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g.
Type:
Application
Filed:
July 30, 2007
Publication date:
August 28, 2008
Applicant:
Novozymes A/S
Inventors:
Kim Vilbour Andersen, Martin Schulein, Torben Henriksen, Lars Christiansen, Bo Damgaard, Claus Von der Osten
Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
Type:
Application
Filed:
March 21, 2008
Publication date:
August 28, 2008
Applicant:
NOVOZYMES, INC.
Inventors:
Kimberly Brown, Paul Harris, Elizabeth Zaretsky, Edward Re, Elena Vlasenko, Keith McFarland, Alfredo Lopez de Leon