Abstract: A method includes reconstituting a lyophilized microbial community. Ribonucleic acid (RNA) is extracted from at least one targeted microbe in the reconstituted microbial community at a plurality of selected time periods. The RNA as extracted at the selected time period is subjected to a multiplex analyzer and determining RNA concentrations at the plurality of selected time periods. An increase in the RNA concentration from a first of the plurality of selected time periods to a second of the plurality of time periods indicates RNA expression from the at least one targeted microbe. RNA expression from the at least one targeted microbe indicates that the at least one targeted microbe is a living microbe.

Abstract: A media supplement for culturing anaerobic bacteria is provided which comprises a filtrate of eilluent from a chemostat vessel in which a target bacterial ecosystem has been culnn-ed. Methods of using the supplement for culturing or isolating anaerobic microbial strains or cormmmities, particularly anaerobic bacteria from the human gut, are also provided.

Abstract: A media supplement for culturing anaerobic bacteria is provided which comprises a filtrate of eilluent from a chemostat vessel in which a target bacterial ecosystem has been culnn-ed. Methods of using the supplement for culturing or isolating anaerobic microbial strains or communities, particularly anaerobic bacteria from the human gut, are also provided.