Abstract: The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity.

Abstract: The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity.

Abstract: The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity.

Abstract: A culture insert carrier for supporting at least one culture insert in a culture tray having at least one well, wherein the culture insert carrier comprises a plane member with at least one opening for insertion of the at least one culture insert in such a way that the culture insert carrier supports the at least one culture insert in a position in the culture tray is provided. Further, a culture insert comprising a plurality of suspension elements including a first set of suspension elements and a second set of suspension elements, the suspension elements being adapted to suspend the culture insert in a plurality of vertical positions, including a first vertical position and a second vertical position, in relation to a frame is provided.

Abstract: The present invention relates to a culture insert carrier, a culture insert and a culture system for culturing and testing of different kinds of cells, such as for example skin models. A culture insert carrier for supporting at least one culture insert in a culture tray having at least one well, wherein the culture insert carrier comprises a plane member with at least one opening for insertion of the at least one culture insert in such a way that the culture insert carrier supports the at least one culture insert in a position in the culture tray is provided. Further, a culture insert comprising a plurality of suspension elements including a first set of suspension elements and a second set of suspension elements, the suspension elements being adapted to suspend the culture insert in a plurality of vertical positions, including a first vertical position and a second vertical position, in relation to a frame is provided.

Abstract: A stack of communicating trays for cultivation of cells, whereby the tray stack is equipped with a gas exchanger having at least one processed aperture for fast and substantially uniform distribution of gas to the trays.

Abstract: The present invention relates to a culture insert carrier, a culture insert and a culture system for culturing and testing of different kinds of cells, such as for example skin models. A culture insert carrier for supporting at least one culture insert in a culture tray having at least one well, wherein the culture insert carrier comprises a plane member with at least one opening for insertion of the at least one culture insert in such a way that the culture insert carrier supports the at least one culture insert in a position in the culture tray is provided. Further, a culture insert comprising a plurality of suspension elements including a first set of suspension elements and a second set of suspension elements, the suspension elements being adapted to suspend the culture insert in a plurality of vertical positions, including a first vertical position and a second vertical position, in relation to a frame is provided.

Abstract: A container and closure assembly, affording a simple, quick, ergonomical, and reliable closing operation, provides an intermediate, partly open position that is reliably indicated. The distance of rotation of the closure relative to the container necessary for closing the closure is short, so that quick and simple closing of the container is provided. The closure is mounted to the container by a projection that is cooperatively received in a track. Either the closure or the container may be provided with the projection, the other being provided with the track. In a preferred embodiment, the track includes a protuberance that hinders the movement of the projection past a predetermined position along the track.

Abstract: Two-dimensional microcarriers for culture of anchorage dependent cells are formed having two opposed and parallel anchorage surfaces and a thickness so thin that cells attach only to the surfaces and not between the surfaces. Preferably, each of the surfaces is less than about 500 microns in each of its two dimensions, the thickness is not more than about 35 microns, the microcarriers have a shape resulting from the orthogonal or oblique intersection of two parallel planes separated by a distance of not more than 35 microns with a solid cylinder and the microcarriers are made from a hydrophobic polymeric material such as polystyrene, polycarbonate or polyterephthalate. The microcarriers can be microdisks having a density of between 0.90 and 1.10 g/cm.sup.3. The anchorage surfaces may be treated to enable cell attachment by applying a thin metallic deposit such as a titanium deposit or by electrical corona discharge. The microcarriers may be transparent to light waves and capable of withstanding sterilization.

Abstract: A method for modifying the surface of a solid polymer wherein the polymer surface is exposed to an aqueous solution containing a two-ring heterocyclic compound that is described in more detail herein. The polymer and the two-ring heterocyclic compound are irradiated with electromagnetic radiation having a wavelength ranging from about 10 nm to about 400 nm to photochemically immobilize the two-ring heterocyclic compound to the polymer.