Abstract: The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity.

Abstract: The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity.

Abstract: The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity.

Abstract: A culture insert carrier for supporting at least one culture insert in a culture tray having at least one well, wherein the culture insert carrier comprises a plane member with at least one opening for insertion of the at least one culture insert in such a way that the culture insert carrier supports the at least one culture insert in a position in the culture tray is provided. Further, a culture insert comprising a plurality of suspension elements including a first set of suspension elements and a second set of suspension elements, the suspension elements being adapted to suspend the culture insert in a plurality of vertical positions, including a first vertical position and a second vertical position, in relation to a frame is provided.

Abstract: The present invention relates to a culture insert carrier, a culture insert and a culture system for culturing and testing of different kinds of cells, such as for example skin models. A culture insert carrier for supporting at least one culture insert in a culture tray having at least one well, wherein the culture insert carrier comprises a plane member with at least one opening for insertion of the at least one culture insert in such a way that the culture insert carrier supports the at least one culture insert in a position in the culture tray is provided. Further, a culture insert comprising a plurality of suspension elements including a first set of suspension elements and a second set of suspension elements, the suspension elements being adapted to suspend the culture insert in a plurality of vertical positions, including a first vertical position and a second vertical position, in relation to a frame is provided.

Abstract: A stack of communicating trays for cultivation of cells, whereby the tray stack is equipped with a gas exchanger having at least one processed aperture for fast and substantially uniform distribution of gas to the trays.

Abstract: The present invention relates to a culture insert carrier, a culture insert and a culture system for culturing and testing of different kinds of cells, such as for example skin models. A culture insert carrier for supporting at least one culture insert in a culture tray having at least one well, wherein the culture insert carrier comprises a plane member with at least one opening for insertion of the at least one culture insert in such a way that the culture insert carrier supports the at least one culture insert in a position in the culture tray is provided. Further, a culture insert comprising a plurality of suspension elements including a first set of suspension elements and a second set of suspension elements, the suspension elements being adapted to suspend the culture insert in a plurality of vertical positions, including a first vertical position and a second vertical position, in relation to a frame is provided.

Abstract: A method for modifying the surface of a solid polymer wherein the polymer surface is exposed to an aqueous solution containing a two-ring heterocyclic compound that is described in more detail herein. The polymer and the two-ring heterocyclic compound are irradiated with electromagnetic radiation having a wavelength ranging from about 10 nm to about 400 nm to photochemically immobilize the two-ring heterocyclic compound to the polymer.