Abstract: Agrobacterium strains which have lost the capacity to proliferate vigorously in vitro or in planta, are provided, as well as transformation methods using these Agrobacterium strains.
Abstract: Obtaining double-haploid cucumber plants from haploid plants is considerably improved when immature or unfertilized ovulae or embryo sac cells, which may be contained in ovarial tissue, are isolated, the formation of callus, embryos or shoots is induced on a hormone-containing medium, these haploid plants are cultured and duplication of the genome is effected.