Abstract: A method for the identification of the presence of E. coli, and Enterococcus faecalis and/or Enterococcus faecium in liquid or liquified samples. The method employs novel oligonucleotide primers based on sequences of the E. coli LamB gene and the Enterococcus faecalis/faecium transposase gene Tn1546. The method involves enrichment of the liquid or liquified sample, preferably using a selective medium, obtaining DNA from bacteria in the sample, and PCR amplification of the DNA using the novel oligonucleotide primers. Kits with the primers are also contemplated.
Abstract: A rapid method for detecting the presence or absence of coliform bacteria in a liquid or liquified dairy sample, for example, skimmed milk, lowfat milk, or whole milk. A growth medium containing a fluorogenic substrate is combined with the dairy sample and is incubated for a brief period of time, for example for about 4 hours, after which a first fluorescence value of 4-methylumbelliferone is measured. The dairy sample is incubated again for about 2-8 hours after which a second fluorescence value of 4-methylumbelliferone is measured. Total, fecal, or thermotolerant coliform bacteria are determined to be present in the sample if the second fluorescent value exceeds the first fluorescent value by a predetermined 4-methylumbelliferone concentration threshold.
Type:
Grant
Filed:
March 29, 2001
Date of Patent:
January 28, 2003
Assignee:
Nye Colifast AS
Inventors:
Ingun Tryland, James D. Berg, Kari Skjanes
Abstract: A rapid method for detecting the presence or absence of coliform bacteria in a liquid or liquified dairy sample, for example skimmed milk. A growth medium containing a fluorogenic substrate is combined with the sample and is incubated for a brief period of about 7-9 hours after which a single fluorescence value is measured. Total or thermotolerant coliform bacteria are determined to be present in the sample if the single fluorescent measurement exceeds a predetermined threshold value.
Type:
Grant
Filed:
August 12, 1999
Date of Patent:
December 26, 2000
Assignee:
NYE Colifast, AS
Inventors:
Gro .O slashed.fjord, Kari Skj.ang.nes, Nina Aalen, James D. Berg