Abstract: An acoustic monitoring system and method for the health status of an offshore wind turbine and an ocean wave are provided. The acoustic monitoring system includes a first laser transmitter, a second laser transmitter, a telephoto camera provided at a hub, a vibration detection sensor provided on a tower of a wind turbine, and four acoustic detection sensors arranged at an interval of 90° along the circumference of the tower. The first and second laser transmitters are arranged at the bottom of a nacelle of the wind turbine and emit laser lights vertically downward. The first laser transmitter, the second laser transmitter, the telephoto camera, the vibration detection sensor, and the acoustic detection sensors are connected to a data acquisition and conversion module through a transmission module. The acoustic monitoring system combines laser light detection with acoustic signal feature detection to improve stability and safety of the offshore wind turbine.

Abstract: The present disclosure discloses a degassing-free underwater dissolved carbon dioxide detection device and a detection method. The degassing-free underwater dissolved carbon dioxide detection device includes a computer, which is used to provide the driving signal and controlling parameters for the power tuning unit; the computer is connected with a laser driving control module and the power tuning unit, respectively; the laser driving control module is connected with a laser; the laser is connected with a photo-isolator; the photo-isolator is connected with a thulium-doped fiber vertical-cavity laser system; the thulium-doped fiber vertical-cavity laser system is connected with a photoacoustic cell system through a fiber collimator; the photoacoustic cell system is connected with a pre-amplifier circuit and a lock-in amplifier in sequence, and the lock-in amplifier is connected with the computer.

Abstract: An anchoring device for adapting to tide level for a wave energy power generation device comprises: a housing, wherein a central main shaft is provided at the center thereof, two ends of the central main shaft are respectively rigidly connected to a volute spring, and the interior of the housing is divided into several independent compartments, including a central compartment accommodating the central main shaft; a shaft lock and a central main gear capable of driving the central main shaft to rotate mounted on the central main shaft; and a plurality of hoisting wheel compartments arranged at equal intervals in a circumferential array around the central main shaft. A hoisting wheel is provided in each of the hoisting wheel compartments, and an anchor chain wound on the hoisting wheel protrudes out of the housing through an opening at the bottom of the hoisting wheel compartment.

Abstract: The present disclosure provides a shield sealing device and a shield sealing method, a pile wall is arranged at a shield receiving opening, the shield sealing device is mounted on the pile wall, and the shield sealing device comprises: an elastic inner ring, an inner wall of the elastic inner ring adapting to an outer wall of a cutter head of a shield tunneling machine; and a plurality of control units, the plurality of control units being mounted on the pile wall, the plurality of control units being arranged in a circumferential direction of the elastic inner ring at intervals, and a driving end of the control part pressing against an outer side of the elastic inner ring so as to extrude or release the elastic inner ring.

Abstract: It is provided a method and system for calculating a turbulent mixing intensity, a computer device and a storage medium. The method comprises: performing denoising preprocess on ocean spatiotemporal coupled shear profile data information obtained by a turbulence profiler; constructing a multi-layer turbulent mixing network with topological attributes, where the network includes nodes and connecting edges; and each node is formed by fusion of four physical properties: time series, scale data, energy local intermittency measure and phase; recognizing a cross-scale transfer process of the turbulent energy according to a presence state of the connecting edges in the network; and performing parametric calculation of the turbulent mixing intensity according to the topological attributes of the multi-layer turbulent mixing network. According to the method, quantitative calculation of a turbulent energy transport intensity in an observation area of a full ocean depth profile is implemented.

Abstract: By establishing effective methods for shrimp 3D cell culture and passage, the present invention provides a technology of continuous shrimp cell culture intended for the establishment of immortalized shrimp cell lines. The present invention provides a preparation method of matrigel for 3D cell culture of shrimp by optimizing an additive proportion of matrigel. The present invention further provides a technology of separation and 3D cell culture of shrimp haemolymph cells, where shrimp haemolymph cells adhere to and grow on the surface of the matrigel in the form of a single round cell and a cell pellet/cellular spheroid, with survival and growth abilities being superior to 2D culture effects. The above technology is achieved by optimizing a formula of complete medium for shrimp cells, selecting the medium as an anticoagulant and a diluent for shrimp haemolymph cells, selecting a 3D culture method for surface-adhered growth in the matrigel.

Abstract: A strain of ciliated protozoan is provided, which has an obvious promoting effect on germination and growth of plant seeds. The ciliated protozoan is Colpoda sp. JH2-5-4, with preservation number of CCTCC NO.C202229. After collecting over 2,000 Colpoda strains from soils across China, the present invention has screened and selected a highly efficient and active strain suitable for use as a microbial fertilizer in plant cultivation. The selected ciliated protozoan can significantly shorten the germination time of plant seeds and improve seedling survival rate.

Abstract: The present disclosure provides an in-situ observation system for a bottom boundary layer (BBL) over a shallow-water cohesive seabed and an arrangement method thereof. It establishes a low-cost and easy-operation hydraulic pile foundation system (2), which can ensure the piling depth to achieve the anti-settling and stability. The stainless-steel sticks are assembled freely to construct the interference-free observation unit (1). As the porous discs are used between the feet of the observation unit (1) and the top of the bottom piles, the observation system can be accurately fixed to the pile foundation. It is thus not limited by the self-weight and can integrate various instruments upon requirement. The components in this system can be easily obtained and conveniently maintained. The present disclosure has the advantages of low-cost and stability, can be widely used for long-term in-situ observation of the BBL.

Abstract: This invention discloses a model test device and method for curtain grouting and excavation of tunnels in high-temperature, water-rich and weak strata, comprising weak strata simulation system, working condition simulation system, curtain grouting system, and monitoring and data acquisition system. The weak strata simulation system is the main box used to fill the soft soil. The working condition simulation system includes a high geothermal simulation system, a water pressure simulation system with stepwise loading of water pressure and volume, and a three-dimensional confining pressure simulation system with stepwise controllable loading of confining pressure. The invention can realize the whole test process simulation of grouting reinforcement and excavation of tunnels in weak strata effectively under the action of confining pressure and geothermal heat, and it has a positive reference effect on actual soft stratum tunnel curtain grouting and safe excavation.

Abstract: An equipment and application method for sinkage detection and active escape of deep-sea mining vehicle, comprising: the sinkage detection and warning devices and the self-escape equipment. The sinkage detection and warning devices are installed on the left and right sides of each track of the deep-sea mining vehicle, and it monitors the contact conditions between the tracks and the sediment, and the distance from the bottom of the track to the device is measured. The self-escape equipment comprises the active interactive grouting system, which connects to intelligent steering and retractable grouting heads. The intelligent steering and retractable grouting heads are also located on the left and right sides of each track. This invention can detect the complex driving environment of the seafloor automatically and combine the existing grouting technology and materials to achieve active grouting escape in case of excessive sinkage.

Abstract: This invention discloses a model test apparatus and method for shield post-wall grouting and tube sheet uplift under high water pressure conditions, comprising main body box, tension apparatus, shield system, grouting system, ground stress loading system, water injection system, and monitoring system. The main body box comprises the shield shell system and model soil inside. The ground stress loading system and water injection system realize the simulation of the complex underground soil and muddy water environment by applying stress and water pressure to the model soil inside the main box. While the tension apparatus makes the shield shell moving forward, the grouting system injects slurry into the gap between the shield shell and the tube sheet. The integrated model test of visual shield grouting and tube sheet uplift, the real-time multi-physical field responses, and the longitudinal deformation mechanism have been realized and revealed under high water pressure conditions.

Abstract: An auto-collapsible pore pressure probe device, including a support system, a penetration system and a measurement system. The support system includes a first support frame, a second support frame, a separation mechanism, a ring clamp, a fixing nut, a fixing bolt, support legs, slots, a support base, and a third support frame. The penetration system includes a rod storage wheel, a motor, a tightening mechanism, a penetration drive motor, a gear, a fixing bracket, a fixing bolt, and a friction wheel. The measurement system includes a pore pressure probe, a control cabinet, a CPTU probe, a pore pressure sensor, a probe connector, an external thread, an internal thread, a fastening strip, a connecting bolt, a connector, a data transmission and power supply cable, a displacement sensor, and a deck unit. An operating method of the pore pressure probe device is also provided.

Abstract: The present invention provides a five-component marine natural gas hydrate intelligent sensing node, comprising a titanium alloy compartment, an information acquisition unit, an integrated control chip, and a power module; the integrated control chip comprises an intelligent computing unit and a transmission unit; the intelligent computing unit is configured for acquiring quality monitoring indicators of marine natural gas hydrates by feature extraction and transmitting reduced represented features to a monitoring device on the sea surface via the transmission unit.

Abstract: The present disclosure belongs to the field of petroleum engineering, and specifically relates to a subsea Christmas tree re-prediction method integrating Kalman filter and Bayesian network. The subsea Christmas tree re-prediction method integrating Kalman filter and Bayesian network includes three steps: digital twin model establishment, degradation process re-prediction model establishment, and remaining useful life calculation model establishment.

Abstract: A method for predicting heaving motion parameters of a semi-submersible offshore platform based on heaving acceleration includes: in heaving motion of a semi-submersible offshore platform, representing heaving acceleration of the semi-submersible offshore platform based on a linear potential flow theory; considering a noise influence of a heaving motion measurement marine environment, a low-frequency influence caused by a slow change of the environment and an influence caused by a baseline drift error of an acceleration sensor, introducing a noise term, a low-frequency change term and a baseline drift error term, and uniformly representing the noise term, the low-frequency change term and the baseline drift error term by a unified Prony sequence; and removing a drift term from uniformly represented heaving acceleration, establishing a relationship between the heaving acceleration and heaving motion parameters in terms of the remaining Prony sequence with the drift term being removed, and estimating the heavin

Abstract: This invention discloses a double-liquid grouting slurry, its technology and application for super large diameter underwater shield engineering under high water pressure condition. The materials of slurry I are: 35-45 parts of cement clinker; 15-25 parts of slag; 24-35 parts of fly ash; 15-25 parts of steel slag; 5-15 parts of bentonite; 4-10 parts of limestone tailing; 0.3-2.0 parts of water reducing agent; 0.5-2.5 parts of cellulose. The materials of slurry II are: 0.2-3.8 parts of short-cut fiber; 96-99 parts of sodium silicate solution; 0.8-4.8 parts of viscous polymers. This invention generates the double-liquid slurry preparation process including crushing-screening-milling-group mixing-grouped mixing at different speeds, the volume ratio of slurry I and II is 1:1-10:1 during grouting, and the slurry is injected into the shield void through the six-point position technology at the shield tail and 3+2+1 segment splicing synchronous grouting techniques.

Abstract: A synchronous single-liquid grouting slurry, its technology and application for large diameter shield engineering under water-rich, high-pressure and weak soil strata conditions, comprising raw materials: 1050-1200 parts of gold tailing, 420-480 parts of silicate cement clinker, 220-240 parts of fly ash, 45-120 parts of waste clay brick, 65-95 parts of slag, 25-45 parts of limestone tailing, 70-80 parts of steel slag, 30-45 parts of silica fume, 15-22 parts of desulfurized gypsum, and 9-15 parts of quick-setting and early-strength composite additive. The invention controls the d50, d85 and d95 of the material particles as 35-40, 42-48 and 50-55 ?m, respectively. Gold tailing with the particle size of 120-600 ?m being used as the fine aggregate, their volume fractions are 40-60%. The slurry production technique, comprising crushing-sieving-superfine ball milling-homogenization-particle size classification-variable speed mixing being developed.

Abstract: A novel PSL family prenyltransferase has relaxed substrate specificity, which can use a variety of cyclic dipeptides and prenyl donors as substrates to produce various terpenylated diketopiperazines. An amino acid sequence of the prenyltransferase is SEQ ID NO: 1. An application of the prenyltransferase is transferring different prenyl groups to Trp-containing cyclic dipeptides. The prenyltransferase catalyzes the formation of terpenylated diketopiperazines by assembling prenyl groups onto cyclic dipeptides, which provides a new strategy for drug development of diketopiperazines.

Abstract: A method for predicting heaving motion parameters of a semi-submersible offshore platform based on heaving acceleration includes: in heaving motion of a semi-submersible offshore platform, representing heaving acceleration of the semi-submersible offshore platform based on a linear potential flow theory; considering a noise influence of a heaving motion measurement marine environment, a low-frequency influence caused by a slow change of the environment and an influence caused by a baseline drift error of an acceleration sensor, introducing a noise term, a low-frequency change term and a baseline drift error term, and uniformly representing the noise term, the low-frequency change term and the baseline drift error term by a unified Prony sequence; and removing a drift term from uniformly represented heaving acceleration, establishing a relationship between the heaving acceleration and heaving motion parameters in terms of the remaining Prony sequence with the drift term being removed, and estimating the heavin

Abstract: The present invention discloses a kelp harvester. The kelp harvester includes a ship body and a U-shaped tripping piece, where two working chain racks are symmetrically arranged on both sides of the ship body; each working chain rack is provided with a chain and a chain driving device used to drive the chain to operate; multiple rope clamping devices are fixedly arranged on each chain; the rope clamping devices can clamp main ropes, so that when the chains operate, the rope clamping devices can drive the ship body to move along with the operation of the chains; the U-shaped tripping piece is rotatablely connected to the ship body through a hinge; and the ship body is further fixedly provided with a limiting baffle, and the limiting baffle limits the U-shaped tripping piece to stopping rotation when the U-shaped tripping piece is in a vertical state.