Abstract: This invention discloses a model test apparatus and method for shield post-wall grouting and tube sheet uplift under high water pressure conditions, comprising main body box, tension apparatus, shield system, grouting system, ground stress loading system, water injection system, and monitoring system. The main body box comprises the shield shell system and model soil inside. The ground stress loading system and water injection system realize the simulation of the complex underground soil and muddy water environment by applying stress and water pressure to the model soil inside the main box. While the tension apparatus makes the shield shell moving forward, the grouting system injects slurry into the gap between the shield shell and the tube sheet. The integrated model test of visual shield grouting and tube sheet uplift, the real-time multi-physical field responses, and the longitudinal deformation mechanism have been realized and revealed under high water pressure conditions.

Abstract: Disclosed is a method for determining 224Ra in a sediment by using a pulse ionization chamber emanometer, which belongs to the technical field of analysis and measurement. A pulse ionization chamber emanometer (PIC), a new emanometer, is used. Based on the half-life characteristics of different radon isotopes, one can separate the 220Rn activity from the total counts by dual counting. The resulting 220Rn measurement then can be used to determine the 224Ra activity in sediment according to the principle of secular radioactive equilibrium.

Abstract: This application relates to a laser-plasma composite cladding head and a composite cladding method, and belongs to the field of surface processing of metallic materials. In the laser-plasma composite cladding head of this application, a ring laser beam is split into two half-ring laser beams through an upper roof lens to avoid a tungsten electrode. The two half-ring laser beams pass through a left 45° reflective lens to be coaxial with the tungsten electrode and then are integrated into a complete ring laser beam through a lower roof lens, thereby allowing a coaxial output of the ring laser beam and a plasma beam. This application integrates the advantages of laser cladding and plasma cladding and adopts coaxial compounding and synchronous powder-feeding.

Abstract: The disclosure discloses a method and application for rapid and accurate chromosomal location of economic traits in laver, belonging to the fields of genomics and molecular breeding, wherein comprising the following steps: distinguishing the different genotype sectors by the color difference of pigment mutants, releasing monospores based on the asexual reproduction of single-genotype sectors, forming offspring CMD population; performing QTL-seq analysis on the extreme phenotype pools of offspring CMD population by SNP/InDel markers; and combining KASP and RNA-seq to predict the location of the genes of discrete traits or major QTL. The disclosure may solve the problem of difficulty in genetic analysis of various traits caused by the genotypic chimeric haploid characteristics.

Abstract: A synchronous single-liquid grouting slurry, its technology and application for large diameter shield engineering under water-rich, high-pressure and weak soil strata conditions, comprising raw materials: 1050-1200 parts of gold tailing, 420-480 parts of silicate cement clinker, 220-240 parts of fly ash, 45-120 parts of waste clay brick, 65-95 parts of slag, 25-45 parts of limestone tailing, 70-80 parts of steel slag, 30-45 parts of silica fume, 15-22 parts of desulfurized gypsum, and 9-15 parts of quick-setting and early-strength composite additive. The invention controls the d50, d85 and d95 of the material particles as 35-40, 42-48 and 50-55 ?m, respectively. Gold tailing with the particle size of 120-600 ?m being used as the fine aggregate, their volume fractions are 40-60%. The slurry production technique, comprising crushing-sieving-superfine ball milling-homogenization-particle size classification-variable speed mixing being developed.

Abstract: Disclosed are an aerogel with a hierarchical pore structure formed using a pulsed laser technology, and a preparation method and use thereof. In the preparation method, a nano silicon-containing inorganic material as a freezing element, a biomass polymer as a cross-linking agent, and deionized water as a solvent are mixed and a resulting mixture is left to stand and gelatinized to obtain a hydrogel; the hydrogel is frozen to form ice crystals therein, and the ice crystals are removed by freeze-drying to obtain a micron-nano porous aerogel; the micron-nano porous aerogel is subjected to customized millimeter-scale punching using a pulsed laser to obtain an aerogel with a millimeter-micron-nano hierarchical pore structure.

Abstract: A method including contacting a target molecule in a sample with a probe, the probe containing a target molecule binding domain capable of specifically binding to the target molecule and at least one reaction blocking modification, carrying out a first processing on a first region of the sample to cause the probe in the first region to be attached to a first tag sequence, carrying out a second processing on a second region that is different from the first region in the sample to cause the probe in the second region to be attached to a second tag sequence, the second tag sequence being different from the first tag sequence, and determining a composition of the probe attached to the first tag sequence and the probe attached to the second tag sequence.

Abstract: The present disclosure discloses a degassing-free underwater dissolved carbon dioxide detection device and a detection method. The degassing-free underwater dissolved carbon dioxide detection device includes a computer, which is used to provide the driving signal and controlling parameters for the power tuning unit; the computer is connected with a laser driving control module and the power tuning unit, respectively; the laser driving control module is connected with a laser; the laser is connected with a photo-isolator; the photo-isolator is connected with a thulium-doped fiber vertical-cavity laser system; the thulium-doped fiber vertical-cavity laser system is connected with a photoacoustic cell system through a fiber collimator; the photoacoustic cell system is connected with a pre-amplifier circuit and a lock-in amplifier in sequence, and the lock-in amplifier is connected with the computer.

Abstract: By establishing effective methods for shrimp 3D cell culture and passage, the present invention provides a technology of continuous shrimp cell culture intended for the establishment of immortalized shrimp cell lines. The present invention provides a preparation method of matrigel for 3D cell culture of shrimp by optimizing an additive proportion of matrigel. The present invention further provides a technology of separation and 3D cell culture of shrimp haemolymph cells, where shrimp haemolymph cells adhere to and grow on the surface of the matrigel in the form of a single round cell and a cell pellet/cellular spheroid, with survival and growth abilities being superior to 2D culture effects. The above technology is achieved by optimizing a formula of complete medium for shrimp cells, selecting the medium as an anticoagulant and a diluent for shrimp haemolymph cells, selecting a 3D culture method for surface-adhered growth in the matrigel.

Abstract: An equipment and application method for sinkage detection and active escape of deep-sea mining vehicle, comprising: the sinkage detection and warning devices and the self-escape equipment. The sinkage detection and warning devices are installed on the left and right sides of each track of the deep-sea mining vehicle, and it monitors the contact conditions between the tracks and the sediment, and the distance from the bottom of the track to the device is measured. The self-escape equipment comprises the active interactive grouting system, which connects to intelligent steering and retractable grouting heads. The intelligent steering and retractable grouting heads are also located on the left and right sides of each track. This invention can detect the complex driving environment of the seafloor automatically and combine the existing grouting technology and materials to achieve active grouting escape in case of excessive sinkage.

Abstract: This invention discloses a model test apparatus and method for shield post-wall grouting and tube sheet uplift under high water pressure conditions, comprising main body box, tension apparatus, shield system, grouting system, ground stress loading system, water injection system, and monitoring system. The main body box comprises the shield shell system and model soil inside. The ground stress loading system and water injection system realize the simulation of the complex underground soil and muddy water environment by applying stress and water pressure to the model soil inside the main box. While the tension apparatus makes the shield shell moving forward, the grouting system injects slurry into the gap between the shield shell and the tube sheet. The integrated model test of visual shield grouting and tube sheet uplift, the real-time multi-physical field responses, and the longitudinal deformation mechanism have been realized and revealed under high water pressure conditions.

Abstract: This invention discloses a model test device and method for curtain grouting and excavation of tunnels in high-temperature, water-rich and weak strata, comprising weak strata simulation system, working condition simulation system, curtain grouting system, and monitoring and data acquisition system. The weak strata simulation system is the main box used to fill the soft soil. The working condition simulation system includes a high geothermal simulation system, a water pressure simulation system with stepwise loading of water pressure and volume, and a three-dimensional confining pressure simulation system with stepwise controllable loading of confining pressure. The invention can realize the whole test process simulation of grouting reinforcement and excavation of tunnels in weak strata effectively under the action of confining pressure and geothermal heat, and it has a positive reference effect on actual soft stratum tunnel curtain grouting and safe excavation.

Abstract: The present disclosure belongs to the field of petroleum engineering, and specifically relates to a subsea Christmas tree re-prediction method integrating Kalman filter and Bayesian network. The subsea Christmas tree re-prediction method integrating Kalman filter and Bayesian network includes three steps: digital twin model establishment, degradation process re-prediction model establishment, and remaining useful life calculation model establishment.

Abstract: A method for predicting heaving motion parameters of a semi-submersible offshore platform based on heaving acceleration includes: in heaving motion of a semi-submersible offshore platform, representing heaving acceleration of the semi-submersible offshore platform based on a linear potential flow theory; considering a noise influence of a heaving motion measurement marine environment, a low-frequency influence caused by a slow change of the environment and an influence caused by a baseline drift error of an acceleration sensor, introducing a noise term, a low-frequency change term and a baseline drift error term, and uniformly representing the noise term, the low-frequency change term and the baseline drift error term by a unified Prony sequence; and removing a drift term from uniformly represented heaving acceleration, establishing a relationship between the heaving acceleration and heaving motion parameters in terms of the remaining Prony sequence with the drift term being removed, and estimating the heavin

Abstract: A synchronous single-liquid grouting slurry, its technology and application for large diameter shield engineering under water-rich, high-pressure and weak soil strata conditions, comprising raw materials: 1050-1200 parts of gold tailing, 420-480 parts of silicate cement clinker, 220-240 parts of fly ash, 45-120 parts of waste clay brick, 65-95 parts of slag, 25-45 parts of limestone tailing, 70-80 parts of steel slag, 30-45 parts of silica fume, 15-22 parts of desulfurized gypsum, and 9-15 parts of quick-setting and early-strength composite additive. The invention controls the d50, d85 and d95 of the material particles as 35-40, 42-48 and 50-55 ?m, respectively. Gold tailing with the particle size of 120-600 ?m being used as the fine aggregate, their volume fractions are 40-60%. The slurry production technique, comprising crushing-sieving-superfine ball milling-homogenization-particle size classification-variable speed mixing being developed.

Abstract: This invention discloses a double-liquid grouting slurry, its technology and application for super large diameter underwater shield engineering under high water pressure condition. The materials of slurry I are: 35-45 parts of cement clinker; 15-25 parts of slag; 24-35 parts of fly ash; 15-25 parts of steel slag; 5-15 parts of bentonite; 4-10 parts of limestone tailing; 0.3-2.0 parts of water reducing agent; 0.5-2.5 parts of cellulose. The materials of slurry II are: 0.2-3.8 parts of short-cut fiber; 96-99 parts of sodium silicate solution; 0.8-4.8 parts of viscous polymers. This invention generates the double-liquid slurry preparation process including crushing-screening-milling-group mixing-grouped mixing at different speeds, the volume ratio of slurry I and II is 1:1-10:1 during grouting, and the slurry is injected into the shield void through the six-point position technology at the shield tail and 3+2+1 segment splicing synchronous grouting techniques.

Abstract: A method for predicting heaving motion parameters of a semi-submersible offshore platform based on heaving acceleration includes: in heaving motion of a semi-submersible offshore platform, representing heaving acceleration of the semi-submersible offshore platform based on a linear potential flow theory; considering a noise influence of a heaving motion measurement marine environment, a low-frequency influence caused by a slow change of the environment and an influence caused by a baseline drift error of an acceleration sensor, introducing a noise term, a low-frequency change term and a baseline drift error term, and uniformly representing the noise term, the low-frequency change term and the baseline drift error term by a unified Prony sequence; and removing a drift term from uniformly represented heaving acceleration, establishing a relationship between the heaving acceleration and heaving motion parameters in terms of the remaining Prony sequence with the drift term being removed, and estimating the heavin

Abstract: The present invention discloses a kelp harvester. The kelp harvester includes a ship body and a U-shaped tripping piece, where two working chain racks are symmetrically arranged on both sides of the ship body; each working chain rack is provided with a chain and a chain driving device used to drive the chain to operate; multiple rope clamping devices are fixedly arranged on each chain; the rope clamping devices can clamp main ropes, so that when the chains operate, the rope clamping devices can drive the ship body to move along with the operation of the chains; the U-shaped tripping piece is rotatablely connected to the ship body through a hinge; and the ship body is further fixedly provided with a limiting baffle, and the limiting baffle limits the U-shaped tripping piece to stopping rotation when the U-shaped tripping piece is in a vertical state.

Abstract: The present disclosure belongs to the field of petroleum engineering, and specifically relates to a subsea Christmas tree re-prediction method integrating Kalman filter and Bayesian network. The subsea Christmas tree re-prediction method integrating Kalman filter and Bayesian network includes three steps: digital twin model establishment, degradation process re-prediction model establishment, and remaining useful life calculation model establishment.

Abstract: Provided is a method for preparing water-soluble astaxanthin complex. The method includes mixing a raw material containing astaxanthin with a solution containing an organic acid and performing cell disruption and leaching and can enable natural astaxanthin to interact with components such as proteins, nucleic acids, or polysaccharides that naturally exist in the raw material, so as to directly prepare water-soluble astaxanthin complex without modifying astaxanthin. The method requires a natural source of astaxanthin, has low equipment requirements and production costs, is simple to operate, green and safe with no organic solvent residues, and easy to industrialize. The representative figure is FIG. 1.