Abstract: The present invention provides a means to identify the alleles present in a DNA-containing sample by providing subsets of loci for amplification by multiplex PCR. The loci include the thirteen CODIS short tandem repeat (STR) loci and amelogenin. The loci within each subset are grouped so that, upon PCR amplification, the amplicons produced within a given subset do not overlap. Differential labeling of subsets makes it possible to further group the subsets into compound multiplexes for co-amplification in a single reaction vessel, and analysis in a single electrophoretic channel.

Abstract: The present invention is directed to an assay system, a kit, and a process for detecting the length of a polymorphic region of a genetic locus by means of bracketing locus compatible or specific calibrating DNA markers upon electrophoresis in a viscous medium.