Abstract: This invention is used for the multiplex analysis method of cells or tissues by combination of multiple cubicles and permeable membrane. Utilizing the diffusion based reagents administration; cells and tissues in multiple cubicles receive signals evenly in their cubicles as parallel manner. This device makes efficient multiplex cells or tissues assays with simple plate layout. According to the molecular permeable mechanism, single spike of stimuli as chemical trigger, light activation, electric triggers or temperature shift can induce multiple cells or tissues response at once. Then accompanying with calibrator molecules as for the navigation of stimuli and assisted by suitable computer simulation, accurate kinetic cells or tissues response analysis is achieved.