Abstract: A method of purifying nucleic acids using solid phases that are modified by a blocking reagent to limit the binding area and/or binding site of the solid phases to reduce the binding capacity of the solid phase to the nucleic acids. The starting amount of the nucleic acids exceeds the binding capacity of the solid phase, and the amount of the purified nucleic acid is in a fix amount, not proportional to the starting amount of the nucleic acids.
Type:
Application
Filed:
February 1, 2024
Publication date:
August 8, 2024
Applicant:
OMEGA BIO-TEK, INC.
Inventors:
Christopher Stuart, Daniel Flanigan, Julie Baggs
Abstract: A unique novel reagent container device with a unique grid structure that allows for the sealing of liquids including volatile liquids. The reagent container device of the present disclosure comprises a container that can be sealed and a support system that forms a grid for sealing the top of the container. Such support system does not restrict liquid flow nor forms an additional section or well within the container device.
Type:
Application
Filed:
February 13, 2023
Publication date:
August 17, 2023
Applicant:
Omega Bio-tek, Inc.
Inventors:
Steven Cupello, Travis Butts, Julie Baggs, Qi Guo
Abstract: The invention provides methods and kits for nucleic acid purification and fragment selection and recovery. By adjusting salt concentrations and/or pH of the binding buffers, only nucleic acid fragments with desired size ranges are able to reversibly and non-specifically bind to a solid surface in certain salt concentrations and/or pH conditions, and can be subsequently eluted and/or recovered from the solid surface in water and/or a low salt eluting buffer.
Abstract: A method of isolation of nucleic acids from a biological sample of cells comprising a combination of a solid phase cell nuclei isolation procedure with a solid phase nucleic acid isolation method.
Abstract: A method of isolation of nucleic acids from a biological sample of cells comprising a combination of a solid phase cell nuclei isolation procedure with a solid phase nucleic acid isolation method.