Abstract: An active/resilient grinding media inside a tube containing a sample is oscillated rapidly by a homogenizer so that the active media is driven in a first direction until it impacts a first end of the tube, which causes it to deform and store an energy charge as it decelerates and stops, and it then accelerates rapidly in the second opposite direction under the discharging force of the stored energy toward the opposite second end of the tube. This cycle of the active media decelerating/charging and then discharging/accelerating is repeated throughout the entire oscillatory processing of the sample. The result is much higher velocities of the active media and therefore much greater impact forces when the sample and active media collide, producing increased efficiency in disruption and size-reduction of the sample particles.

Abstract: An active/resilient grinding media inside a tube containing a sample is oscillated rapidly by a homogenizer so that the active media is driven in a first direction until it impacts a first end of the tube, which causes it to deform and store an energy charge as it decelerates and stops, and it then accelerates rapidly in the second opposite direction under the discharging force of the stored energy toward the opposite second end of the tube. This cycle of the active media decelerating/charging and then discharging/accelerating is repeated throughout the entire oscillatory processing of the sample. The result is much higher velocities of the active media and therefore much greater impact forces when the sample and active media collide, producing increased efficiency in disruption and size-reduction of the sample particles.

Abstract: An active/resilient grinding media inside a tube containing a sample is oscillated rapidly by a homogenizer so that the active media is driven in a first direction until it impacts a first end of the tube, which causes it to deform and store an energy charge as it decelerates and stops, and it then accelerates rapidly in the second opposite direction under the discharging force of the stored energy toward the opposite second end of the tube. This cycle of the active media decelerating/charging and then discharging/accelerating is repeated throughout the entire oscillatory processing of the sample. The result is much higher velocities of the active media and therefore much greater impact forces when the sample and active media collide, producing increased efficiency in disruption and size-reduction of the sample particles.

Abstract: Methods and systems of analyzing a biological sample to detect the presence of a virus are disclosed, that include mechanically homogenizing the biological sample, wherein the biological sample is unprocessed, to lyse the unprocessed biological sample to produce a homogenized sample; amplifying the viral nucleic acids in the homogenized sample, where such amplification occurs without any isolation, separation, purification, or extraction of the viral nucleic acids in the homogenized sample; and analyzing the amplified viral nucleic acids to detect the presence of the virus.

Abstract: An ultrasonic system for homogenizing a sample includes an ultrasonic transducer and a control system. The transducer includes a horn or other probe that oscillates to produce cavitation in the sample and a converter that drives the horn through its oscillatory motion. The control system includes a closed-loop amplitude-control configuration and process. In particular, the control system includes a user interface, a controller, and a high-frequency driver, all connected together in a closed-loop configuration for enabling amplitude-control feedback. Control software includes programming for the closed-loop amplitude-control process including receiving a user-inputted desired amplitude of oscillatory horn motion, driving the transducer at a corresponding power level, determining the actual amplitude of oscillatory horn motion, and automatically adjusting the power level to the transducer to maintain the desired amplitude during operation of the ultrasonic system.

Abstract: An active/resilient grinding media inside a tube containing a sample is oscillated rapidly by a homogenizer so that the active media is driven in a first direction until it impacts a first end of the tube, which causes it to deform and store an energy charge as it decelerates and stops, and it then accelerates rapidly in the second opposite direction under the discharging force of the stored energy toward the opposite second end of the tube. This cycle of the active media decelerating/charging and then discharging/accelerating is repeated throughout the entire oscillatory processing of the sample. The result is much higher velocities of the active media and therefore much greater impact forces when the sample and active media collide, producing increased efficiency in disruption and size-reduction of the sample particles.

Abstract: A mounting apparatus for holding tubes of samples for processing by a homogenizer. The mounting apparatus includes a processing plate and at least one cassette, with the plate for mounting to the homogenizer, the cassette for mounting to the plate, and the tube for holding by the cassette. In some embodiments, the cassette includes one or more tube-retention assemblies for removably securing the tube against axial, lateral, and/or rotational movement, for example, using centrifugal forces of the homogenizer for retention assistance. In some embodiments, the cassette is removably mounted to the plate, for example, using centrifugal forces of the homogenizer for retention assistance. And in some embodiments, the tube can be replaced in the cassette with the cassette mounted to the plate adjacent an access opening through which the tube can pass, while centrifugal forces assist in retaining the tubes in the cassette during operation of the homogenizer.

Abstract: An ultrasonic system for homogenizing a sample includes an ultrasonic transducer and a control system. The transducer includes a horn or other probe that oscillates to produce cavitation in the sample and a converter that drives the horn through its oscillatory motion. The control system includes a closed-loop amplitude-control configuration and process. In particular, the control system includes a user interface, a controller, and a high-frequency driver, all connected together in a closed-loop configuration for enabling amplitude-control feedback. Control software includes programming for the closed-loop amplitude-control process including receiving a user-inputted desired amplitude of oscillatory horn motion, driving the transducer at a corresponding power level, determining the actual amplitude of oscillatory horn motion, and automatically adjusting the power level to the transducer to maintain the desired amplitude during operation of the ultrasonic system.

Abstract: A processing plate assembly for mounting to a homogenizer and for holding tubes containing samples to be homogenized. The processing plate assembly includes a mounting structure and one or plural tube holders. The mounting structure removably or permanently mounts to the homogenizer and can be provided by for example a flat plate-like mounting structure. The tube holders are attached to the mounting structure and hold the tubes in generally tangential use positions with a centroid of each tube positioned along a longitudinal axis of the tube and axially offset from a radius line of the processing plate that is perpendicular to the tube axis. In this way, the homogenizer imparts sinusoidal swashing forces on the tubes that urge the tubes forward into securement in their tube holders and that produces improved homogenization in the tubes.

Abstract: A tube holder for mounting a sample-tube assembly to a homogenizing device includes at least one clamp, at least one retainer, and a mount. The mount couples the tube holder to the homogenizing device. Each clamp defines a tube channel and a transverse ledge surface, with the tube channel having a lateral opening through which a tube of the tube assembly can be easily inserted and removed for example with one hand. And each retainer defines a retaining surface that extends over the respective tube channel and opposes the respective ledge surface to cooperatively form a receptacle that receives/retains a cap of the tube assembly against axial tube motion during homogenization. In some embodiments, multiple retaining surfaces are included for retaining different types of tube assemblies, multiple clamps and retainers are included for holding multiple tube assemblies simultaneously, and/or ejection mechanisms are included for ease of ejecting the tube assemblies.

Abstract: Agitation mechanisms for homogenization devices for processing sample materials in tubes that are secured by tube holders to the agitation mechanisms. Each agitation mechanism includes a first rotary member having a first fixed rotational axis, a second rotary member having a second fixed rotational axis, and a connecting member that extends between them, is rotationally mounted to them at third and fourth non-fixed rotational axes, and to which the tube holder is mounted, with the first and third rotational axes defining a first offset, and with the second and fourth rotational axes defining a second offset. When the first rotary member is driven through rotation, the sample in the tube in the tube holder on the connecting member is driven through a nonlinearly reciprocating motion profile to produce a grinding shear action to better homogenize the samples. Other disclosed embodiments produce linearly reciprocating motion profiles.

Abstract: A flow disrupter in a tube chamber of a tube assembly for homogenizing sample materials includes a flow-disrupting body that extends generally transversely into the tube chamber and divides the tube chamber into two sub-chambers. The flow-disrupting body includes at least one narrowed flow passageway through which the sample flows back and forth in both axially reciprocating directions as the tube assembly is vigorously shaken at high speeds faster and more reliably than what can be accomplished by hand shaking. And the flow-disrupting body includes at least two flow-interrupting surfaces facing generally in opposite axial directions and against which the sample impacts in each respective axially reciprocating direction as the tube assembly is vigorously shaken. In this way, the vigorous high-speed shaking of the tube assembly including the flow disrupter results in significant particle-size reduction of the sample by mechanical shear, fluid shear, cavitation, and/or pressure differentials.

Abstract: A processing plate assembly for mounting to a homogenizer and for holding tubes containing samples to be homogenized. The processing plate assembly includes a mounting structure and one or plural tube holders. The mounting structure removably or permanently mounts to the homogenizer and can be provided by for example a flat plate-like mounting structure. The tube holders are attached to the mounting structure and hold the tubes in generally tangential use positions with a centroid of each tube positioned along a longitudinal axis of the tube and axially offset from a radius line of the processing plate that is perpendicular to the tube axis. In this way, the homogenizer imparts sinusoidal swashing forces on the tubes that urge the tubes forward into securement in their tube holders and that produces improved homogenization in the tubes.

Abstract: Agitation mechanisms for homogenization devices for processing sample materials in tubes that are secured by tube holders to the agitation mechanisms. Each agitation mechanism includes a first rotary member having a first fixed rotational axis, a second rotary member having a second fixed rotational axis, and a connecting member that extends between them, is rotationally mounted to them at third and fourth non-fixed rotational axes, and to which the tube holder is mounted, with the first and third rotational axes defining a first offset, and with the second and fourth rotational axes defining a second offset. When the first rotary member is driven through rotation, the sample in the tube in the tube holder on the connecting member is driven through a nonlinearly reciprocating motion profile to produce a grinding shear action to better homogenize the samples. Other disclosed embodiments produce linearly reciprocating motion profiles.

Abstract: A tube holder for mounting a sample-tube assembly to a homogenizing device includes at least one clamp, at least one retainer, and a mount. The mount couples the tube holder to the homogenizing device. Each clamp defines a tube channel and a transverse ledge surface, with the tube channel having a lateral opening through which a tube of the tube assembly can be easily inserted and removed for example with one hand. And each retainer defines a retaining surface that extends over the respective tube channel and opposes the respective ledge surface to cooperatively form a receptacle that receives/retains a cap of the tube assembly against axial tube motion during homogenization. In some embodiments, multiple retaining surfaces are included for retaining different types of tube assemblies, multiple clamps and retainers are included for holding multiple tube assemblies simultaneously, and/or ejection mechanisms are included for ease of ejecting the tube assemblies.

Abstract: A flow disrupter in a tube chamber of a tube assembly for homogenizing sample materials includes a flow-disrupting body that extends generally transversely into the tube chamber and divides the tube chamber into two sub-chambers. The flow-disrupting body includes at least one narrowed flow passageway through which the sample flows back and forth in both axially reciprocating directions as the tube assembly is vigorously shaken at high speeds faster and more reliably than what can be accomplished by hand shaking. And the flow-disrupting body includes at least two flow-interrupting surfaces facing generally in opposite axial directions and against which the sample impacts in each respective axially reciprocating direction as the tube assembly is vigorously shaken. In this way, the vigorous high-speed shaking of the tube assembly including the flow disrupter results in significant particle-size reduction of the sample by mechanical shear, fluid shear, cavitation, and/or pressure differentials.

Abstract: A flow disrupter in a tube chamber of a tube assembly for homogenizing sample materials includes a flow-disrupting body that extends generally transversely into the tube chamber and divides the tube chamber into two sub-chambers. The flow-disrupting body includes at least one narrowed flow passageway through which the sample flows back and forth in both axially reciprocating directions as the tube assembly is vigorously shaken at high speeds faster and more reliably than what can be accomplished by hand shaking. And the flow-disrupting body includes at least two flow-interrupting surfaces facing generally in opposite axial directions and against which the sample impacts in each respective axially reciprocating direction as the tube assembly is vigorously shaken. In this way, the vigorous high-speed shaking of the tube assembly including the flow disrupter results in significant particle-size reduction of the sample by mechanical shear, fluid shear, cavitation, and/or pressure differentials.

Abstract: A mounting apparatus for holding tubes of samples for processing by a homogenizer. The mounting apparatus includes a processing plate and at least one cassette, with the plate for mounting to the homogenizer, the cassette for mounting to the plate, and the tube for holding by the cassette. In some embodiments, the cassette includes one or more tube-retention assemblies for removably securing the tube against axial, lateral, and/or rotational movement, for example, using centrifugal forces of the homogenizer for retention assistance. In some embodiments, the cassette is removably mounted to the plate, for example, using centrifugal forces of the homogenizer for retention assistance. And in some embodiments, the tube can be replaced in the cassette with the cassette mounted to the plate adjacent an access opening through which the tube can pass, while centrifugal forces assist in retaining the tubes in the cassette during operation of the homogenizer.

Abstract: A combination fin-type mixer and rotor/stator-type homogenizer provides the ability to perform low-shear mixing or high-shear homogenizing by a single device without changing attachments or hardware. The low-shear mixing fins extend outwardly from the stator of the high-shear rotor/stator homogenizer, and the rotor rotates within the stator. For high-shear homogenizing, the rotor is rotated within the stator. And for low-shear mixing, the finned stator is selectively interlocked to and rotated with the rotor. A method of combining components includes resetting the device from low-shear mixing mode to high-shear homogenizing mode, and vice versa, without changing attachments or hardware.

Abstract: A plastic disruption and homogenization device. The device has rotor that is axially disposed within a stator. The stator and the rotor have several teeth to aid the disruption and homogenization process. The device consists of plastic material that allows the rotor to rotate smoothly without the use of bearings.