Patents Assigned to On-Chip Biotechnologies Co., Ltd
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Publication number: 20240091775Abstract: One object of the present invention is to provide a method or apparatus for purifying target particles from high-concentration particles in a short time. The above problem can be solved by a method for purifying target particles, characterized in that the method comprises a step of sorting the target particles from a high concentration of non-target particles, wherein the sorting step is repeated for three times or more.Type: ApplicationFiled: November 28, 2023Publication date: March 21, 2024Applicant: ON-CHIP BIOTECHNOLOGIES CO., LTD.Inventors: Kazuo TAKEDA, Masayuki ISHIGE, Yuu FUJIMURA, Takahide INO, Yuji MORISHITA, Kosuke OSAWA, Soichiro TSUDA
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Publication number: 20230383240Abstract: An object of the present invention is to purify and concentrate differentiating cells derived from ES cells, iPS cells, or the like without damaging them.Type: ApplicationFiled: August 15, 2023Publication date: November 30, 2023Applicant: ON-CHIP BIOTECHNOLOGIES CO., LTD.Inventors: Kazuo TAKEDA, Yuu FUJIMURA, Takahide INO, Masayuki ISHIGE, Jin AKAGI
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Patent number: 11826752Abstract: One object of the present invention is to provide a method or apparatus for purifying target particles from high-concentration particles in a short time. The above problem can be solved by a method for purifying target particles, characterized in that the method comprises a step of sorting the target particles from a high concentration of non-target particles, wherein the sorting step is repeated for three times or more.Type: GrantFiled: August 24, 2021Date of Patent: November 28, 2023Assignee: ON-CHIP BIOTECHNOLOGIES CO., LTD.Inventors: Kazuo Takeda, Masayuki Ishige, Yuu Fujimura, Takahide Ino, Yuji Morishita, Kosuke Osawa, Soichiro Tsuda
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Publication number: 20230295700Abstract: A microfluidic nucleic acid detection kit and a detection device are provided. The microfluidic nucleic acid detection kit is provided includes a kit body and a reagent card interconnected. The kit body can slide relative to the reagent card to control the kit to work. The microfluidic nucleic acid detection kit further includes a gas source cavity, a liquid cavity, a nucleic acid amplification cavity, and a gas tail cavity. The gas tail cavity communicates with the nucleic acid amplification cavity through an exhaust gas flow channel. The exhaust gas flow channel is provided with a check valve. The detection device includes the microfluidic nucleic acid detection kit, a first housing, a power module, a display module, a first heating module, and a first detection module. The microfluidic nucleic acid detection kit is removably inserted into the first housing.Type: ApplicationFiled: May 10, 2022Publication date: September 21, 2023Applicant: CHENGDU ONE-CHIP BIOTECHNOLOGY CO., LTD.Inventors: Zhifeng ZHANG, Jianlong LI
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Patent number: 11725179Abstract: An object of the present invention is to purify and concentrate differentiating cells derived from ES cells, iPS cells, or the like without damaging them.Type: GrantFiled: May 12, 2016Date of Patent: August 15, 2023Assignee: ON-CHIP BIOTECHNOLOGIES CO., LTD.Inventors: Kazuo Takeda, Yuu Fujimura, Takahide Ino, Masayuki Ishige, Jin Akagi
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Patent number: 11371984Abstract: An apparatus for analyzing particles in a solution includes a unit configured to place a flow cell having a flow path for flowing a sample solution containing the particles; a unit configured to illuminate the sample solution flowing through the flow path of the flow cell; a photodetector that detects a scattered light and/or fluorescence generated from the particles in the sample solution; and a unit configured to analyze the particles based on their signal intensities detected by the photodetector, wherein the flow cell has the flow path formed in a substrate, a reflection plane is formed on the side surface of the flow path, the reflection plane leads the lights generated in the flow path of the flow cell and advancing in the substrate in-plane direction to a specified region of the surface of the flow cell, and the photodetector detects the light exiting from the specified region to the outside.Type: GrantFiled: April 6, 2019Date of Patent: June 28, 2022Assignee: ON-CHIP BIOTECHNOLOGIES CO., LTD.Inventor: Kazuo Takeda
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Patent number: 11280792Abstract: The object of the present invention is to provide an evaluation method capable of accurately determining a metastasis of cancer, the stage of cancer progression, or the malignancy of cancer.Type: GrantFiled: September 6, 2018Date of Patent: March 22, 2022Assignees: ON-CHIP BIOTECHNOLOGIES CO., LTD., SHIZUOKA PREFECTUREInventors: Kazuo Takeda, Namiko Yamashita, Yuu Fujimura, Kaori Nishio, Yasuhiro Koh, Masaru Watanabe, Fumiaki Koizumi, Yuri Uehara
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Publication number: 20220062905Abstract: One object of the present invention is to provide a method or apparatus for purifying target particles from high-concentration particles in a short time. The above problem can be solved by a method for purifying target particles, characterized in that the method comprises a step of sorting the target particles from a high concentration of non-target particles, wherein the sorting step is repeated for three times or more.Type: ApplicationFiled: August 24, 2021Publication date: March 3, 2022Applicant: ON-CHIP BIOTECHNOLOGIES CO., LTD.Inventors: Kazuo TAKEDA, Masayuki ISHIGE, Yuu FUJIMURA, Takahide INO, Yuji MORISHITA, Kosuke OSAWA, Soichiro TSUDA
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Patent number: 11002660Abstract: The present invention provides an apparatus for analyzing particles in a solution including a unit configured to place a flow cell having a flow path for flowing a sample solution containing the particles; a unit configured to illuminate the sample solution flowing through the flow path of the flow cell; a photodetector that detects a scattered light and/or fluorescence generated from the particles in the sample solution; and a unit configured to analyze the particles based on their signal intensities detected by the photodetector, wherein the flow cell has the flow path formed in a substrate, a reflection plane is formed on the side surface of the flow path, the reflection plane leads the lights generated in the flow path of the flow cell and advancing in the substrate in-plane direction to a specified region of the surface of the flow cell, and the photodetector detects the light exiting from the specified region to the outside.Type: GrantFiled: April 16, 2018Date of Patent: May 11, 2021Assignee: ON-CHIP BIOTECHNOLOGIES CO., LTD.Inventor: Kazuo Takeda
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Publication number: 20200256785Abstract: A disposable flow cell formed on a substrate for separating particles from a sample solution includes a sample flow path and a sample reservoir formed on the substrate, wherein the sample flow path is connected to the sample reservoir for introducing the sample solution from the sample reservoir, the sample flow path comprises an illuminating region configured for illumination by an external illumination unit, wherein the sample reservoir is adapted to be airtightly connected to an external constant air pump such that a constant air pressure can be applied to the sample solution by the external constant air pump; and a first branched flow path and a second branched flow path perpendicularly connected, respectively, to both sides of the sample flow path at a location downstream of the illumination region, wherein the first branched flow path comprises a connection port configured for air-tight connection to an external pulse air pump.Type: ApplicationFiled: May 2, 2020Publication date: August 13, 2020Applicant: ON-CHIP BIOTECHNOLOGIES CO., LTD.Inventor: Kazuo Takeda
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Patent number: 10724938Abstract: The object of the present invention is to provide (1) a cell sorter, (2) a flow cytometer capable of detecting sideward scattered light, (3) a method for accurately measuring cell concentration, (4) a method for multicolor staining analysis without a fluorescence correction, and the like, which satisfy requirements that carry-over and cross contamination of samples do not occur.Type: GrantFiled: September 7, 2018Date of Patent: July 28, 2020Assignee: ON-CHIP BIOTECHNOLOGIES CO., LTD.Inventor: Kazuo Takeda
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Patent number: 10648899Abstract: A method for sorting a particle of interest in a solution includes storing a sample solution in a sample solution reservoir and applying a constant pressure to the sample solution; illuminating the sample solution flowing in an illumination region of the sample solution flow path upstream of a branched flow path region, and detecting the particle of interest contained in the sample solution; and upon detecting the particle of interest, opening an electromagnetic valve or electromagnetic valves that are disposed between an air pressure pump and the branched flow path region of the sample solution flow path at a timing that the detected particle of interest reaches the branched flow path region such that a pulse pressure is applied to the branched flow path region to separate the detected particle of interest from the sample solution flow path.Type: GrantFiled: November 17, 2018Date of Patent: May 12, 2020Assignee: ON-CHIP BIOTECHNOLOGIES CO., LTD.Inventor: Kazuo Takeda
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Publication number: 20190339189Abstract: The object of the present invention is to provide a technique for dispensing cells as single cells. The above problem can be solved by an apparatus for dispensing particles comprising a transparent hollow pipette for dispensing sample liquid containing particles, and an image capturing means, wherein the devise has means for capturing two or more images of the dispensing liquid in the hollow pipette; means for comparing two or more shot images, distinguishing a moved particle-like substance from an unmoved particle-like substance in the image-captured particle-like substances, and identifying the moved particle-like substance as a suspended particle; and means for dispensing a sample liquid containing a target number of suspended particles.Type: ApplicationFiled: September 15, 2017Publication date: November 7, 2019Applicant: ON-CHIP BIOTECHNOLOGIES CO., LTD.Inventors: Kazuo TAKEDA, Yuu FUJIMURA, Masayuki ISHIGE, Takahide INO, Yohsuke BANSHO, Jin AKAGI, Kosuke OSAWA
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Publication number: 20190234861Abstract: An apparatus for analyzing particles in a solution includes a unit configured to place a flow cell having a flow path for flowing a sample solution containing the particles; a unit configured to illuminate the sample solution flowing through the flow path of the flow cell; a photodetector that detects a scattered light and/or fluorescence generated from the particles in the sample solution; and a unit configured to analyze the particles based on their signal intensities detected by the photodetector, wherein the flow cell has the flow path formed in a substrate, a reflection plane is formed on the side surface of the flow path, the reflection plane leads the lights generated in the flow path of the flow cell and advancing in the substrate in-plane direction to a specified region of the surface of the flow cell, and the photodetector detects the light exiting from the specified region to the outside.Type: ApplicationFiled: April 6, 2019Publication date: August 1, 2019Applicant: ON-CHIP BIOTECHNOLOGIES CO., LTD.Inventor: Kazuo Takeda
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Publication number: 20190154563Abstract: A method for sorting a particle of interest in a solution includes storing a sample solution in a sample solution reservoir and applying a constant pressure to the sample solution; illuminating the sample solution flowing in an illumination region of the sample solution flow path upstream of a branched flow path region, and detecting the particle of interest contained in the sample solution; and upon detecting the particle of interest, opening an electromagnetic valve or electromagnetic valves that are disposed between an air pressure pump and the branched flow path region of the sample solution flow path at a timing that the detected particle of interest reaches the branched flow path region such that a pulse pressure is applied to the branched flow path region to separate the detected particle of interest from the sample solution flow path.Type: ApplicationFiled: November 17, 2018Publication date: May 23, 2019Applicant: ON-CHIP BIOTECHNOLOGIES CO., LTD.Inventor: Kazuo Takeda
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Patent number: 10267721Abstract: The present invention provides an apparatus for analyzing particles in a solution including a unit configured to place a flow cell having a flow path for flowing a sample solution containing the particles; a unit configured to illuminate the sample solution flowing through the flow path of the flow cell; a photodetector that detects a scattered light and/or fluorescence generated from the particles in the sample solution; and a unit configured to analyze the particles based on their signal intensities detected by the photodetector, wherein the flow cell has the flow path formed in a substrate, a reflection plane is formed on the side surface of the flow path, the reflection plane leads the lights generated in the flow path of the flow cell and advancing in the substrate in-plane direction to a specified region of the surface of the flow cell, and the photodetector detects the light exiting from the specified region to the outside.Type: GrantFiled: October 27, 2015Date of Patent: April 23, 2019Assignee: On-Chip Biotechnologies Co., Ltd.Inventor: Kazuo Takeda
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Publication number: 20190078995Abstract: The object of the present invention is to provide (1) a cell sorter, (2) a flow cytometer capable of detecting sideward scattered light, (3) a method for accurately measuring cell concentration,(4) a method for multicolor staining analysis without a fluorescence correction, and the like, which satisfy requirements that carry-over and cross contamination of samples do not occur.Type: ApplicationFiled: September 7, 2018Publication date: March 14, 2019Applicant: ON-CHIP BIOTECHNOLOGIES CO., LTD.Inventor: Kazuo TAKEDA
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Patent number: 10222317Abstract: A method for sorting a cell particle in a solution includes steps of preparing an apparatus having a sample solution reservoir formed on a substrate, a main flow path formed in the substrate, a first branched flow path and a second branched flow path, and a downstream flow path; storing a sample solution; applying a constant gas pressure to the sample solution, illuminating the sample solution; determining calculating a delayed timing; and applying a pushing pressure.Type: GrantFiled: October 27, 2015Date of Patent: March 5, 2019Assignee: On-Chip Biotechnologies Co., LtdInventor: Kazuo Takeda
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Publication number: 20190011450Abstract: The object of the present invention is to provide an evaluation method capable of accurately determining a metastasis of cancer, the stage of cancer progression, or the malignancy of cancer.Type: ApplicationFiled: September 6, 2018Publication date: January 10, 2019Applicants: ON-CHIP BIOTECHNOLOGIES CO., LTD., SHIZUOKA PREFECTUREInventors: Kazuo TAKEDA, Namiko YAMASHITA, Yuu FUJIMURA, Kaori NISHIO, Yasuhiro KOH, Masaru WATANABE, Fumiaki KOIZUMI, Yuri UEHARA
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Publication number: 20180298324Abstract: An object of the present invention is to purify and concentrate differentiating cells derived from ES cells, iPS cells, or the like without damaging them.Type: ApplicationFiled: May 12, 2016Publication date: October 18, 2018Applicant: ON-CHIP BIOTECHNOLOGIES CO., LTD.Inventors: Kazuo TAKEDA, Yuu FUJIMURA, Takahide INO, Masayuki ISHIGE, Jin AKAGI