Abstract: The present invention refers to a microfluidic system based on passive capillary valves and pumps, that allows a discontinuous and autonomous measurement process for extensive periods of time. The microfluidic system comprises: at least one measuring chamber, at least one inlet for the input of a biofluid, a microfluidic intake channel fluidly communicating the inlet with the measuring chamber, at least one sensor suitable for measuring a parameter of an analyte of a biofluid. A passive fluid pump is fluidly communicated with the measuring chamber, and it is adapted to generate a capillary pressure greater than the biofluid generation pressure. A retention valve is interposed between the measuring chamber and the fluid pump, and the retention passive valve is configured to stop flow of biofluid for a certain period of time, when the measuring chamber if filled with biofluid.