Abstract: A composition and method for staining cellular DNA are disclosed. The composition of the present invention is an aqueous alcoholic solution that includes a cationic stain, a metabisulfite, and an alcohol preferably selected from methanol, ethanol, and mixtures thereof. In a preferred embodiment, the cationic stain is thionin.

Abstract: A method for detecting malignancy-associated changes. A sample of cells is obtained and stained to identify the nuclear DNA material. The sample is imaged with a digital microscope. Objects of interest are identified in the sample of cells based on the intensity of the pixels that comprise the object versus the average intensity of all pixels in the slide image. An exact edge is located for each object and variations in the illumination intensity of the microscope are compensated for. A computer system calculates feature values for each object and, based on the value of the features, a determination is made whether the cell exhibits malignancy-associated changes or not.

Abstract: Apparatus for manually controlling a motorized microscope stage for movement about the X, Y and Z axes. The apparatus includes a main housing having an X axis control knob and a Y axis control knob rotatably mounted to the housing. A Z axis control knob is provided for controlling movement about the Z axis. The Z axis knob is mounted to a removable module so that the position of the Z axis control knob is adjustable with respect to the other knobs. There is a controller for generating stage control signals in response to movement of the knobs. A plug-in connector connects the apparatus to the microscope stage controller such that the stage control signals create corresponding movements of the motorized stage. The apparatus simulates the controls of a nonmotorized stage while maintaining compatibility with existing stage controller units. The apparatus is designed to provide tactile position feedback to the user similar to that of a manual non-motorized microscope.