Patents Assigned to Oncu Limited
  • Detection of methylated DNA
    Patent number: 9766200
    Abstract: The use of ion sensitive field effect transistor (ISFET) to detect methylated nucleotides in a DNA sample is described. A method of detecting methylated nucleotides in a DNA sample may include the steps of treating a sample of DNA with a reagent which discriminates between methylated and non-methylated nucleotides to provide treated DNA, amplifying the treated DNA and optionally sequencing the amplified DNA. An ISFET is used to monitor the addition of one or more dNTPs in the strand extension reactions during the amplification and/or sequencing step. Suitable apparatus is also provided.
    Type: Grant
    Filed: February 23, 2015
    Date of Patent: September 19, 2017
    Assignee: ONCU LIMITED
    Inventors: Christofer Toumazou, Melpomeni Kalofonou
  • Detection of methylated DNA
    Patent number: 8986981
    Abstract: The use of ion sensitive field effect transistor (ISFET) to detect methylated nucleotides in a DNA sample is described. A method of detecting methylated nucleotides in a DNA sample may include the steps of treating a sample of DNA with a reagent which discriminates between methylated and non-methylated nucleotides to provide treated DNA, amplifying the treated DNA and optionally sequencing the amplified DNA. An ISFET is used to monitor the addition of one or more dNTPs in the strand extension reactions during the amplification and/or sequencing step. Suitable apparatus is also provided.
    Type: Grant
    Filed: March 14, 2011
    Date of Patent: March 24, 2015
    Assignee: Oncu Limited
    Inventors: Christofer Toumazou, Melpomeni Kalofonou