Abstract: The invention provides methods of analyzing a secreted protein from a cell encapsulated in a microdrop. The microdrop is formulated with biotinylated matrix molecules at a reduced ratio of biotin to matrix molecules compared with previous formulations. The reduced ratio is advantageous for improving the resolution of detection and allows simultaneous detection of multiple secreted proteins and/or multiple cell surface markers. The invention further provides inter alia methods of isolating IgG isotype antibodies that have switched from IgM isotype.

Abstract: The present invention provides nucleic acid hybridization probes having a target-binding region and a labeled hairpin structure at at least one end of the probe. The hairpin-labeled probes include oligonucleotides, dendrimers, and primer-extended nucleic acids. The probes can be used in disclosed methods for detection of target nucleic acids. In addition, the oligonucleotide probes can be used in disclosed methods for primer-extension, including, e.g., random priming and PCR amplification, to produce the primer-extended hairpin-labeled probes. Also disclosed are kits comprising the hairpin-labeled oligonucleotide and dendrimer probes. Further, the present invention provides biomolecules (e.g., peptides, polypeptides, carbohydrates, lipids, and the like) that are labeled via linkage to labeled hairpin structures.

Abstract: The invention provides methods of analzying a secreted protein from a cell encapsulated in a microdrop. The microdrop is formulated with biotinylated matrix molecules at a reduced ratio of biotin to matrix molecules compared with previous formulations. The reduced ratio is advantageous for improving the resolution of detection and allows simultaneous detection of multiple secreted proteins and/or multiple cell surface markers. The invention further provides inter alia methods of isolating IgG isotype antibodies that have switched from IgM isotype.

Abstract: The invention provides methods of analzying a secreted protein from a cell encapsulated in a microdrop. The microdrop is formulated with biotinylated matrix molecules at a reduced ratio of biotin to matrix molecules compared with previous formulations. The reduced ratio is advantageous for improving the resolution of detection and allows simultaneous detection of multiple secreted proteins and/or multiple cell surface markers. The invention further provides inter alia methods of isolating IgG isotype antibodies that have switched from IgM isotype.