Abstract: A method providing next generation sequencing (NGS)-based high-resolution HLA typing as a routine clinical test. The method uses a multiplex PCR primer design for amplifying multiple human leukocyte antigen (HLA) Class I and Class II genes in a single reaction for NGS. The test quality is improved and the protocol for typing multiple HLA genes is simplified because the number of amplification reactions are reduced nearly 10-fold, to yield a substantially equimolar ratio of individual HLA gene amplification products. The invention eliminates an amplicon pooling step, reducing the reagent cost, and required sample DNA quantity.
Abstract: The invention is directed to engineered Fc gamma receptor type III (Fc?II, HNA-1) polypeptides and use of these polypeptides to detect antibodies specific for human neutrophil antigens (HNA). The invention is also directed to methods for the diagnosing and determining susceptibility for developing Transfusion Reaction Acute Lung (TRALI).
Type:
Grant
Filed:
February 5, 2020
Date of Patent:
October 4, 2022
Assignee:
One Lambda, Inc.
Inventors:
Jar-How Lee, Neng Jen Remi Shih, Julie Nguyen, Rui Pei
Abstract: The invention is directed to engineered Fc gamma receptor type III (Fc?111, HNA-1) polypeptides and use of these polypeptides to detect antibodies specific for human neutrophil antigens (HNA). The invention is also directed to methods for the diagnosing and determining susceptibility for developing Transfusion Reaction Acute Lung (TRALI).
Type:
Grant
Filed:
May 7, 2015
Date of Patent:
February 25, 2020
Assignee:
ONE LAMBDA, INC.
Inventors:
Jar-How Lee, Neng Jen Remi Shih, Julie Nguyen, Rui Pei
Abstract: The invention provides methods for detecting target nucleic acid sequences with diagnostic probes including first and second probe regions that are substantially complementary to first and second target regions respectively on a target nucleic acid strand wherein the first probe region is located 5? to the second probe region. The first probe region is substantially complementary to the first target region, on the target nucleic acid strand, which also includes a second target region, wherein when the first target region is contiguous with the second target region on the target nucleic acid strand, then the first and second probe regions on the diagnostic probe are separated by a spacer region of nucleic acid.
Abstract: Provided herein are compositions comprising native and denatured human leukocyte antigens (HLA) and methods of making said compositions. Also provided herein are methods and kits for the detection of antibodies to native HLAs.
Type:
Grant
Filed:
December 23, 2014
Date of Patent:
May 31, 2016
Assignee:
ONE LAMBDA, INC.
Inventors:
Paul Terasaki, Adam Idica, Chun-Tsan Deng
Abstract: Provided herein are compositions comprising native and denatured human leukocyte antigens (HLA) and methods of making said compositions. Also provided herein are methods and kits for the detection of antibodies to native HLAs.
Type:
Grant
Filed:
February 16, 2011
Date of Patent:
January 6, 2015
Assignee:
One Lambda, Inc.
Inventors:
Adam Idica, Chun-Tsan Deng, Paul I. Terasaki
Abstract: The present invention relates to methods for screening for binding interactions using multiple sets of microparticles, wherein said set has the same identifiable characteristic and wherein one of more sets comprise subsets of microparticles and said subset presents at least one unique probe that acts as a binding partner for a target molecule in a biological sample. In particular, the invention provides for methods of detecting tissue-typing antigens in donor tissue or recipient tissue using these multiple sets of microparticles.
Abstract: Provided herein are compositions comprising native and denatured human leukocyte antigens (HLA) and methods of making said compositions. Also provided herein are methods and kits for the detection of antibodies to native HLAs.
Type:
Application
Filed:
February 16, 2011
Publication date:
August 18, 2011
Applicant:
One Lambda, Inc.
Inventors:
Adam Idica, Chun-Tsan Deng, Paul I. Terasaki
Abstract: The present invention relates to methods for screening for binding interactions using multiple sets of microparticles, wherein said set has the same identifiable characteristic and wherein one of more sets comprise subsets of microparticles and said subset presents at least one unique probe that acts as a binding partner for a target molecule in a biological sample. In particular, the invention provides for methods of detecting tissue-typing antigens in donor tissue or recipient tissue using these multiple sets of microparticles.