Abstract: A system for producing a library of oligomer comprising at least two monomers in a positionally determined array comprise a plurality of synthesis supports, a first plurality of support carriers wherein each carrier has a uniform array of distinct support holding positions for the synthesis supports; means for contacting each array of synthesis supports with a different monomer to provide a first chemical transformation of the synthesis supports; a second plurality of support carriers wherein each carrier has a uniform array of distinct support holding positions for receiving the chemically transformed synthesis supports contained in the first plurality of support carriers; transfer apparatus for transferring a selected row or column of synthesis supports from each of the first plurality of carriers to each of the second plurality of carriers to enable the supports in the second to undergo at least a second chemical transformation; and whereby each support position in each carrier identifies the chemical comp

Abstract: A system for producing a library of oligomer comprising at least two monomers in a positionally determined array comprise a plurality of synthesis supports, a first plurality of support carriers wherein each carrier has a uniform array of distinct support holding positions for the synthesis supports; means for contacting each array of synthesis supports with a different monomer to provide a first chemical transformation of the synthesis supports; a second plurality of support carriers wherein each carrier has a uniform array of distinct support holding positions for receiving the chemically transformed synthesis supports contained in the first plurality of support carriers; transfer apparatus for transferring a selected row or column of synthesis supports from each of the first plurality of carriers to each of the second plurality of carriers to enable the supports in the second to undergo at least a second chemical transformation; and whereby each support position in each carrier identifies the chemical comp

Abstract: A multiple channel high throughput purification system for purifying a plurality of samples, preferably four or more samples from a chemical library. The high throughput purification uses chromatography, and more preferably, super critical fluid chromatography. Four parallel channels are provided in this system and coupled to a common analyzer and computer. The four channels direct the selected sample flow through a separator, such as an SFC column, a detector, such as a UV detector, to detect peaks within the sample flow, and a micro sample valve that splits a sampling of the flow to an analyzer, such as a mass spectrometer. The system also utilizes unique back pressure regulator assemblies and pressure relief assemblies to maintain a selected pressure within the purification channel. While the sample flow continues, the mass spectrometer simultaneously analyzes the sampling to determine if a target compound is within the sample portion.

Abstract: Y—X—C(R′)═C(R″)COOR″′  (A1)

Abstract: A high throughput liquid chromotagraphy column assembly for separating a large sample with a selected mass weight and fluid volume. The assembly includes a loading column with a loading chamber with a first inner diameter and a first length. The loading chamber contains a packing material on to which sample is loaded and spatially distributed. The length of the loading chamber's volume is sufficient to fully load the sample therein but the length of the loading chamber is insufficient to achieve the selected chromotagraphic separation. A separation column is in fluid communication with the loading column. The separation column has a separation chamber with a diameter smaller than the loading column's inner diameter and a length greater than the loading column's length. In one embodiment, the loading column has a diameter two or more times greater than the separation chamber's diameter, and the loading column has a length one-half or less than the length of the separation chamber.

Abstract: A wash station assembly and method for washing selected samples in a sample containing assembly. The wash station assembly includes a wash station having a frame and a fluid dispensing assembly connected to the frame. The fluid dispensing assembly has a selector valve that is connectable to a plurality of fluid lines and is adjustable to allow only one of the fluids to pass through the selector valve at a time. An array of syringes is coupled to the selector valve and positioned to receive the fluid passing through the selector valve. Each syringe includes a check valve that prevents backflow of fluid or air into the syringe through the pipetting needles. A distribution manifold is connected to the selector valve and receives the fluid passing through the selector valve. The manifold has a plurality of distribution channels coupled to the syringes, to direct the fluid into the syringes. Each distribution channel has a manifold valve therein that prevents backflow of fluid into the manifold from the syringes.

Abstract: The present invention provides novel compounds, novel compositions, methods of their use, and methods of their manufacture, where such compounds are pharmacologically useful inhibitors of Protein Tyrosine Phosphatases (PTPase's) such as PTP1B. CD45, SHP-1, SHP-2, PTP&agr;, LAR and HePTP or the like. The compounds are useful in the treatment of type I diabetes, type II diabetes, impaired glucose tolerance, insulin resistance, obesity, immune dysfunctions including autoimmunity diseases with dysfunctions of the coagulation system, allergic diseases including asthma, osteoporosis, proliferative disorders including cancer and psoriasis, diseases with decreased or increased synthesis or effects of growth hormone, diseases with decreased or increased synthesis of hormones or cytokines that regulate the release of/or response to growth hormone, diseases of the brain including Alzheimer's disease and schizophrenia, and infectious diseases.

Abstract: Disclosed are novel compounds, novel compositions, methods of their use, and methods of their manufacture, where such compounds of Formula 1 are pharmacologically useful inhibitors of Protein Tyrosine Phosphatases (PTPase's) including PTP1B, T cell PTP, wherein n, m, X, R1, R2, R3, R4, R5, R6, and R7 are defined more fully in the description. The compounds are useful in the treatment of type I diabetes, type II diabetes, impaired glucose tolerance, insulin resistance, obesity, and other diseases.

Abstract: Y—X—C(R′)═C(R″)COOR′″  (A1) The present invention relates to novel protein tyrosine phosphatase modulating compounds having the general structure shown in Formula (A1), to methods for their preparation, to compositions comprising the compounds, to their use for treatment of human and animal disorders, to their use for purification of proteins or glycoproteins, and to their use in diagnosis. The invention relates to modulation of the activity of molecules with phosphotyrosine recognition units, including protein tyrosine phosphatases (PTPases) and proteins with Src-homology-2 domains, in in vitro systems, microorganisms, eukaryoic cells, whole animals and human beings. R′ and R″ are independently selected from the group consisting of hydrogen, halo, cyano, nitro, trihalomethyl, alkyl, arylalkyl.

Abstract: A pressure regulator assembly usable in, as an example, a multiple channel high throughput purification system. The pressure regulator assembly includes an inlet line and an outlet line connectable to a fluid channel. A regulator body has a regulator inlet connected to the inlet line and a regulator outlet connected to the outlet line. The regulator body has a chamber therein in fluid communication with the regulator inlet and outlet. A nozzle is in fluid communication with the regulator inlet and has a nozzle outlet adjacent to the chamber. A stem is axially aligned with the nozzle outlet and has a regulating surface adjacent to the nozzle outlet to restrict the fluid flow through the chamber to the regulator outlet. A mounting rod is attached to a mounting portion on the stem. The mounting rod and stem are axially moveable in the regulator body relative to the nozzle outlet.

Abstract: A microsampling device useable in a multiple channel high throughput purification system for purifying a plurality of samples, preferably four or more samples from a chemical library. One aspect of the invention provides a microsampling device for use in the high throughput fluid system. The microsampling device includes a body with a sample flow inlet, a sample flow outlet, and a sample passageway there between. The sample flow inlet and outlet are positionable for fluid communication with the sample flow path of the fluid system. The body has a carrier flow inlet and a carrier flow outlet positioned before fluid communication with the carrier fluid flow path of the high throughput fluid system. The carrier flow inlet and carrier flow outlet are axially misaligned. A stem is movably disposed in the body and in fluid communication with the sample passageway. The stem is moveable between first and second positions.

Abstract: A fraction collection assembly having a frame and a dispensing head movably connected to the frame for movement about three axes. The dispensing head has a dispensing tube that receives and dispenses the sample portion. The dispensing head is movable between a dispensing position, a rinse position, and a pickup position. A receiving container with a receiving well is positioned to receive the sample portion of the selected sample dispensed from the dispensing tube when the dispensing head is in the dispensing position. The receiving well has a 1-to-1 corresponding position to the well from which the sample is originally taken from the supplying container. The rinse station is connected to the frame and positioned to removably receive the dispensing tube when the dispensing head is in the rinse position.

Abstract: A multiple channel high throughput purification system for purifying a plurality of samples, preferably four or more samples from a chemical library. The high throughput purification uses chromatography, and more preferably, super critical fluid chromatography. Four parallel channels are provided in this system and coupled to a common analyzer and computer. The four channels direct the selected sample flow through a separator, such as an SFC column, a detector, such as a UV detector, to detect peaks within the sample flow, and a micro sample valve that splits a sampling of the flow to an analyzer, such as a mass spectrometer. The system also utilizes unique back pressure regulator assemblies and pressure relief assemblies to maintain a selected pressure within the purification channel. While the sample flow continues, the mass spectrometer simultaneously analyzes the sampling to determine if a target compound is within the sample portion.

Abstract: The present invention provides novel compounds, novel compositions, methods of their use, and methods of their manufacture, where such compounds are pharmacologically useful inhibitors of Protein Tyrosine Phosphatases (PTPase's) such as PTP1B, CD45, SHP-1, SHP-2, PTP&agr;, LAR and HePTP or the like. The compounds are useful in the treatment of type I diabetes, type II diabetes, impaired glucose tolerance, insulin resistance, obesity, immune dysfunctions including autoimmunity diseases with dysfunctions of the coagulation system, allergic diseases including asthma, osteoporosis, proliferative disorders including cancer and psoriasis, diseases with decreased or increased synthesis or effects of growth hormone, diseases with decreased or increased synthesis of hormones or cytokines that regulate the release of/or response to growth hormone, diseases of the brain including Alzheimer's disease and schizophrenia, and infectious diseases.

Abstract: A docking station includes remotely actuated locking mechanisms for secure registration of reaction blocks, and provides for introduction of gases, liquids, and vacuum to the reaction blocks.

Abstract: Compounds of formula A are disclosed: ##STR1## wherein R.sup.1 is aralkyl or cycloalkyl; R.sup.2 is cycloalkylmethyl, alkyl, or aralkyl;R.sup.3 is hydrogen, alkyl, substituted phenyl (including p-phenoxy-phenyl), or fluorene;R.sup.4 is hydrogen, alkyl, substituted phenyls (including 1-alkyl-4-carboxy-substituted phenyls), alkyl carboxylic acids;R.sup.5 is hydrogen or R.sup.1 and R.sup.5 taken together forming a tetrahydroisoquinoline ring or a piperidine ring.These compounds exhibit inhibitory action against fructose-1,6-bisphosphatase (FBPase) and are indicated in the treatment or management of Type II diabetes.

Abstract: A wash station assembly and method for washing selected samples in a sample containing assembly. The wash station assembly includes a wash station having a frame and a fluid dispensing assembly connected to the frame. The fluid dispensing assembly has a selector valve that is connectable to a plurality of fluid lines and is adjustable to allow only one of the fluids to pass through the selector valve at a time. An array of syringes is coupled to the selector valve and positioned to receive the fluid passing through the selector valve. Each syringe includes a check valve that prevents backflow of fluid or air into the syringe through the pipetting needles. A distribution manifold is connected to the selector valve and receives the fluid passing through the selector valve. The manifold has a plurality of distribution channels coupled to the syringes, to direct the fluid into the syringes. Each distribution channel has a manifold valve therein that prevents backflow of fluid into the manifold from the syringes.

Abstract: Y--X--C(R').dbd.C(R")COOR"'(A1)The present invention relates to novel protein tyrosine phosphatase modulating compounds having the general structure shown in Formula (A1), to methods for their preparation, to compositions comprising the compounds, to their use for treatment of human and animal disorders, to their use for purification of proteins or glycoproteins, and to their use in diagnosis. The invention relates to modulation of the activity of molecules with phosphotyrosine recognition units, including protein tyrosine phosphatases (PTPases) and proteins with Src-homology-2 domains, in in vitro systems, microorganisms, eukaryoic cells, whole animals and human beings. R' and R" are independently selected from the group consisting of hydrogen, halo, cyano, nitro, trihalomethyl, alkyl, arylalkyl. R"' is selected from the group consisting of hydrogen, alkyl, substituted alkyl, aryl, arylalkyl. X is aryl. Y is selected from hydrogen or ##STR1## wherein (*) indicates a potential point of attachment to X.

Abstract: There are disclosed methods useful for the inhibition of inducible nitric oxide synthase by the adminstration of a compound of the formula I:. when R.sub.2 is H then R.sub.1 is selected from the group consisting of:1) aryl,--NH--C.sub.0 -C.sub.10 alkyl -aryl, N-(C.sub.0 -C.sub.10 alkyl substituted aryl).sub.2, --C.sub.0 -C.sub.10 alkyl substituted aryl, --N(C.sub.0 -C.sub.10 alkyl substituted aryl)(C.sub.0 -C.sub.

Abstract: The present invention relates to polysubsituted imidazole having formula 1 ##STR1## These compounds are useful for restoring the sensitivity of multidrug resistant cells to cancer chemotherapeutic agents.