Abstract: An intraocular injection system for injecting a substance into an eye globe of an animal or human eye, the system comprising an injection device with a piercing member for injection of the substance into the eye globe, the intraocular injection system providing an automated piercing of the eye globe by the piercing member. The system further comprises a robotic manipulator carrying the injection device, the robotic manipulator adapted and configured to move the injection device to a pre-injection position, a detection device adapted and configured to detect a position of a patch placed onto the eye globe and to control the robotic manipulator to move the injection device to the pre-injection position, the pre-injection position being based upon the detected position of the patch on the eye globe.