Abstract: A method of forming a column (100 and 200) for use with an analytical chemical instrument. The method includes placing a frit (102 and 202) in proximity to a distal end (110 and 210) of a tube (104 and 204) having an internal bore (106 and 206) adapted to receive packing material (108 and 208) for selectively interacting with an analyte of interest in a sample. The method further includes laser welding the frit to the tube and inserting packing material within the internal bore of the tube. A column formed in accordance with this method.

Abstract: A device for centrifugation and robotic manipulation of specimen samples, including incubating eggs, and uses thereof are provided. The device may advantageously be used for the incubation of avian, reptilian or any type of vertebrate eggs. The apparatus comprises a mechanism for holding samples individually, rotating them individually, rotating them on a centrifuge collectively, injecting them individually with a fixative or other chemical reagent, and maintaining them at controlled temperature, relative humidity and atmospheric composition. The device is applicable to experiments involving entities other than eggs, such as invertebrate specimens, plants, microorganisms and molecular systems.

Abstract: A bioreactor apparatus and cell culturing system is provided for the automated cultivation and processing of living cells remotely both on earth and in low gravity which utilizes a generally cylindrical reactor vessel that may be optionally rotated about its cylindrical axis while allowing the entrance of fresh or recycled fluid and the removal, optionally, of spent medium, medium to be recycled or filtered or unfiltered medium for the collection of samples. A method of exchanging gases between the culture medium and ambient gases is provided. A fresh-medium storage bag and peristaltic pump is used for batch feeding, perfusion or sample collection. An enclosure and manifold representing an additional level of chemical containment and a series of pinch valves for the periodic collection of samples of suspended cells or cell-free medium is disposed therein together with a humidity control system.

Abstract: The present invention relates to improved techniques for separating cells, particles and molecules important to medical science and biotechnology because separation is frequently the limiting factor for many biological processes. The apparatus and method of use provides an innovative method for quantitatively separating cells, proteins, or other particles, using multistage, magnetically, electromagnetically assisted separation technology, (“MAGSEP”). The MAGSEP technology provides a separation technology applicable to medical, chemical, cell biology, and biotechnology processes. Moreover, the instant invention relates to a method for separating and isolating mixtures of combinatorial synthesized molecules such that a variety of products are prepared, in groups, possessing diversity in size, length, (molecular weight), and structural elements. These are then analyzed for the ability to bind specifically to an antibody, receptor, or other ligate.

Abstract: A quarter turn quick connect fitting assembly (10) for connecting miniature fluid conduits, such as tubing, includes a first fitting (12) having radially projecting pins (28) and a second fitting (14) located within a rotatable quarter turn nut (16) having spiral grooves (48) which receive the pins. The first fitting defines a central passage (26) and the second fitting defines a central passage (42) which may each be configured to slidably receive a hollow tube set (104), or to threadably receive an externally threaded mating fitting. The quarter turn quick connect fitting can be configured to attach to the end of a column, and can include an adapter (158), which houses a guard column (160), or a frit (188) for filtering the fluid passing through the quarter turn quick connect fitting assembly.

Abstract: A quarter turn quick connect fitting assembly (10) for connecting miniature fluid conduits, such as tubing, includes a first fitting (12) having radially projecting pins (28) and a second fitting (14) located within a rotatable quarter turn nut (16) having spiral grooves (48) which receive the pins. The first fitting defines a central passage (26) and the second fitting defines a central passage (42) which may each be configured to slidably receive a hollow tube set (104), or to threadably receive an externally threaded mating fitting. The quarter turn quick connect fitting can be configured to attach to the end of a column, and can include an adapter (158), which houses a guard column (160), or a frit (188) for filtering the fluid passing through the quarter turn quick connect fitting assembly. The quarter turn quick connect fitting is assembled by sliding the pins of the first fitting into the grooves of the quarter turn nut and rotating the quarter turn nut through approximately 90.degree.

Abstract: A pump head quick connect assembly (10) includes a pump head (28) having a body (30) penetrated by a first locating pin (16), having a proximal end (18) and a distal end (20), and a second locating pin (22), having a proximal end (24) and a distal end (26). The proximal ends of the first and second locating pins are attached to a base plate (12). The first locating pin distal end and the second locating pin distal end project beyond the pump head body and are inserted into a first outer passage (48) and a second outer passage (50), respectively, defined by a face plate (70). The face plate further defines an internally-threaded center passage (52) that threadably receives a manually adjustable screw (38). As the center screw is tightened against the pump head body, the pump head body is forced against the base plate, thereby forming a seal and centering the pump head on the base plate to prevent cocking.

Abstract: An integral fitting and filter assembly includes a tubular body (119) having a integral proximal fitting piece (121) and an integral distal fitting piece (122). A central passage (125) extends through the proximal fitting piece and the distal fitting piece of the body. A tubular filter assembly sleeve including a filter sub-assembly (142) and tubular extension sleeve (140) is slidably received within the central passage of the body. The filter sub-assembly includes two end caps (144, 145) and a center piece (146). The center piece is packed with a selective chemical-absorbent packing material (26), that is retained within the center piece by first and second porous plugs (148) secured within opposite ends of the center piece of the end caps.

Abstract: An integral fitting and filter assembly includes a tubular body (119) having a integral proximal fitting piece (121) and an integral distal fitting piece(122). A central passage (125) extends through the proximal fitting piece and the distal fitting piece of the body. A tubular filter assembly sleeve including a filter sub-assembly (142) and tubular extension sleeve (140) is slidably received within the central passage of the body. The filter sub-assembly includes two end caps (144, 145) and a center piece (146). The center piece is packed with a selective chemical-absorbent packing material (26), that is retained within the center piece by first and second porous plugs (148) secured within opposite ends of the center piece of the end caps.

Abstract: A fitting assembly (10) mates an end of polymer micro-tubing (12) in fluid communication with an internally threaded connector (22) having an internal bottom wall (38). The fitting assembly includes a male nut (16) having external threads and a passage for receiving the end of the polymer micro-tubing. The fitting assembly additionally includes an insert (24) having an elongated stem (30) and an annular head (26). The stem projects from the rear side of the annular head for insertion into the end of the polymer micro-tubing. The annular head includes a forwardly projecting nose section (31). An elastomeric seal (40) is received on the nose section (31) and retained in place by a lip (33) included on the nose section. The seal is compressed between the head of the insert and the bottom wall of the connector.

Abstract: A valve (10) is used to introduce fluid from a syringe (22) into the inlet (88) of the pump of an HPLC instrument (12). The valve includes a valve body (14) that is connected to the HPLC instrument for fluid flow communication with the instrument inlet. A valve rotor (16) is rotatably engaged with the valve body. A collar fitting (18) included on the valve rotor is connectable to the syringe. The syringe acts as a valve lever, and is displaceable to rotate the valve rotor about an axis of rotation (32) between an "off" position, in which fluid flow between the syringe and the HPLC instrument inlet is prevented, and an "on" position, in which fluid flow between the syringe and the HPLC instrument inlet is permitted. The valve enables one-handed priming operation.

Abstract: An integral fitting and filter assembly (10) includes a tubular body (12) having a integral proximal fitting end (14) and an integral distal fitting end (16). A central passage (18) extends from the proximal fitting end to the distal fitting end of the body. A tubular sleeve (20) is slidably received within the central passage of the body. The tubular sleeve includes a stem portion (22) and a larger diameter head portion (24). The stem portion of the sleeve is packed with a selective chemical-absorbent packing material (26), that is retained within the sleeve by first and second porous plugs (28) secured within opposite ends of the stem portion of the sleeve. The sleeve is slidably inserted into the body, and is adjustably positioned longitudinally to provide a pre-column filter that may be used to mate with differently configured mating fittings, such as high-performance liquid chromatography instrument inlet fittings.