Abstract: The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.
Type:
Application
Filed:
March 17, 2014
Publication date:
May 19, 2016
Applicant:
OPX Biotechnologies, Inc.
Inventors:
Hans LIAO, Christopher Patrick MERCOGLIANO, Travis Robert WOLTER, Michael Tai Man LOUIE, Wendy Kathleen RIBBLE, Tanya LIPSCOMB, Eileen Colie SPINDLER, Michael LYNCH
Abstract: The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.
Type:
Application
Filed:
March 17, 2014
Publication date:
April 30, 2015
Applicant:
OPX Biotechnologies, Inc.
Inventors:
Hans LIAO, Christopher Patrick MERCOGLIANO, Travis Robert WOLTER, Michael Tai Man LOUIE, Wendy Kathleen RIBBLE, Tanya LIPSCOMB, Eileen Colie SPINDLER, Michael D LYNCH
Abstract: The present invention relates to methods, systems and compositions, including genetically modified microorganisms, directed to achieve decreased microbial conversion of 3-hydroxypropionic acid (3-HP) to aldehydes of 3-HP. In various embodiments this is achieved by disruption of particular aldehyde dehydrogenase genes, including multiple gene deletions. Among the specific nucleic acids that are deleted whereby the desired decreased conversion is achieved are aldA, aldB, puuC), and usg of E. coli. Genetically modified microorganisms so modified are adapted to produce 3-HP, such as by approaches described herein.
Type:
Application
Filed:
May 12, 2014
Publication date:
March 12, 2015
Applicant:
OPX Biotechnologies, Inc.
Inventors:
Michael D. Lynch, Christopher P. Mercogliano, Matthew L. Lipscomb, Tanya E. W. Lipscomb
Abstract: The present invention relates to methods, systems and compositions, including genetically modified microorganisms, adapted to exhibit increased tolerance to 3-hydroxypropionic acid (3-HP), particularly through alterations to interrelated metabolic pathways identified herein as the 3-HP toleragenic pathway complex (“3HPTGC”). In various embodiments these organisms are genetically modified so that an increased 3-HP tolerance is achieved. Also, genetic modifications may be made to provide at least one genetic modification to any of one or more 3-HP biosynthesis pathways in microorganisms comprising one or more genetic modifications of the 3HPTGC.
Type:
Application
Filed:
February 12, 2014
Publication date:
February 26, 2015
Applicants:
The Regents of the University of Colorado, OPX Biotechnologies, Inc.
Inventors:
TANYA E. W. LIPSCOMB, MICHAEL D. LYNCH, RYAN T. GILL
Abstract: Bio-based renewable 3-hydroxypropionic acid (3-HP) may be produced through fermentation processes utilizing genetically modified microorganisms such as, for example, genetically modified E. coli strains. The practice of the invention may include cultivating or culturing (meant to be synonymous) cells or genetically modified microorganisms, including in large-scale fermentation.
Abstract: This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes 3-hydroxypropionic acid.
Type:
Grant
Filed:
September 27, 2010
Date of Patent:
November 11, 2014
Assignees:
OPX Biotechnologies, Inc., The Regents of the University of Colorado, a Body Corporate
Inventors:
Michael D. Lynch, Ryan T. Gill, Tanya E. W. Lipscomb
Abstract: A process for producing high purity 3-hydroxypropionic acid from a fermentation cell broth is described. The 3-hydroxypropionic acid can be converted to a variety of products, such as acrylamide, 3-hydroxypropionic esters, acrylic esters, and 3-HP amide. This process features a high degree of product flexibility, limited or no solvent recycle, discrete waste streams, an efficient water removal process, and efficient recovery of products and solvents with proven and scalable equipment.
Abstract: Microorganism compositions are described that comprise combinations of genetic modifications that include a genetic modification to increase oxaloacetate alpha-decarboxylase enzymatic activity. By such genetic modification a 3-hydroxypropionic acid (“3-HP”) production pathway is provided or improved. In various embodiments, comprising other genetic modifications, including selected gene disruptions, 3-HP production is greater than in a control microorganism lacking such combinations of genetic modifications.
Type:
Grant
Filed:
September 27, 2010
Date of Patent:
August 19, 2014
Assignee:
OPX Biotechnologies, Inc.
Inventors:
Michael D Lynch, Christopher P. Mercogliano
Abstract: Embodiments herein generally relate to methods, compositions, systems and uses for enabling bio-production of or increasing bio-production of alcohol molecules by microorganisms. Certain embodiments relate to compositions and methods enabling or increasing the bio-production of 4-carbon alcohol molecules by bacteria. In some embodiments, compositions and methods relate to introducing isobutyryl-CoA isomerase to a culture of microorganisms to enable or increase the bio-production of four-carbon alcohols. Variations of biosynthesis pathways for microbial bio-production of butanol and/or isobutanol are provided.
Abstract: Methods of obtaining mutant nucleic acid sequences that demonstrate elevated oxaloacetate a-decarboxylase activity are provided. Compositions, such as genetically modified microorganisms that comprise such mutant nucleic acid sequences, are described, as are methods to obtain the same.
Abstract: Three biosynthetic pathways are disclosed for microorganism bio-production of 1,4-Butanediol from various carbon sources. Exemplary methods are provided. The recombinant microorganisms comprising any of these 1,4-Butanediol biosynthesis pathways may also comprise genetic modifications directed to improved tolerance for 1,4-Butanediol.
Abstract: This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a fatty acid or fatty acid derived product, wherein the modified microorganism produces fatty acyl-CoA intermediates via a malonyl-CoA dependent but malonyl-ACP independent mechanism
Type:
Application
Filed:
August 9, 2013
Publication date:
February 20, 2014
Applicant:
OPX Biotechnologies, Inc.
Inventors:
Hans Liao, Eileen Spindler, Joseph R. Warner, Michael Louie, Wendy Ribble, Brittany Prather, Ron Evans, Tanya E.W. Lipscomb, Michael D. Lynch
Abstract: Methods of obtaining mutant nucleic acid sequences that demonstrate elevated oxaloacetate ?-decarboxylase activity are provided. Compositions, such as genetically modified microorganisms that comprise such mutant nucleic acid sequences, are described, as are methods to obtain the same.
Abstract: This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes 3-hydroxypropionic acid.
Type:
Application
Filed:
June 12, 2013
Publication date:
February 13, 2014
Applicants:
THE REGENTS OF THE UNIVERSITY OF COLORADO, OPX BIOTECHNOLOGIES, INC.
Inventors:
Michael D. LYNCH, Ryan T. GILL, Tanya E.W. LIPSCOMB
Abstract: A process for producing high purity 3-hydroxypropionic acid from a fermentation cell broth is described. The 3-hydroxypropionic acid can be converted to a variety of products, such as acrylamide, 3-hydroxypropionic esters, acrylic esters, and 3-HP amide. This process features a high degree of product flexibility, limited or no solvent recycle, discrete waste streams, an efficient water removal process, and efficient recovery of products and solvents with proven and scalable equipment.
Abstract: This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes 3-hydroxypropionic acid.
Type:
Application
Filed:
September 27, 2010
Publication date:
March 21, 2013
Applicant:
OPX Biotechnologies, Inc.
Inventors:
Michael D. Lynch, Ryan T. Gill, Tanya E.W. Lipscomb
Abstract: The present invention relates to methods, systems and compositions, including genetically modified microorganisms, adapted to exhibit increased tolerance to 3-hydroxypropionic acid (3-HP), particularly through alterations to interrelated metabolic pathways identified herein as the 3-HP toleragenic pathway complex (“3HPTGC”). In various embodiments these organisms are genetically modified so that an increased 3-HP tolerance is achieved. Also, genetic modifications may be made to provide at least one genetic modification to any of one or more 3-HP biosynthesis pathways in microorganisms comprising one or more genetic modifications of the 3HPTGC.
Type:
Application
Filed:
March 9, 2012
Publication date:
October 18, 2012
Applicants:
The Regents of the University of Colorado, a body corporate, OPX Biotechnologies, Inc.
Inventors:
Tanya E.W. Lipscomb, Michael D. Lynch, Ryan T. Gill
Abstract: Methods of obtaining mutant nucleic acid sequences that demonstrate elevated oxaloacetate ?-decarboxylase activity are provided. Compositions, such as genetically modified microorganisms that comprise such mutant nucleic acid sequences, are described, as are methods to obtain the same.
Abstract: Methods of obtaining mutant nucleic acid sequences that demonstrate elevated oxaloacetate ?-decarboxylase activity are provided. Compositions, such as genetically modified microorganisms that comprise such mutant nucleic acid sequences, are described, as are methods to obtain the same.
Abstract: This invention relates to microorganism cells that are modified to increase conversion of carbon dioxide and/or carbon monoxide to a product, such as a fatty acid methyl ester, and to related methods and systems. A pathway from the Calvin Benson Cycle to the product is provided, which in various embodiments involves use of heterologous proteins that exhibit desired enzymatic conversions.