Abstract: Methods for assaying properties in tissues or organs of drugs and other chemical compounds and substances include ex vivo normothermic perfusion with a fluid containing a test substance to obtain data regarding the tissue or organ, the substance and/or an interaction of the substance and the tissue or organ. The data can be used as, for example, part of a submission to a government regulatory organization.

Abstract: An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. The method includes perfusing the organ at hypothermic and/or normothermic temperatures. The methods further include perfusing the organ at hypothermic and/or normothermic temperatures with a second perfusate containing a substance for reacting with the organ. Condition of the organ may be automatically monitored, and the perfusion process can be automatically controlled using a control program.

Abstract: An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. The method includes perfusing the organ at hypothermic and/or normothermic temperatures, preferably after hypothermic organ flushing for organ transport and/or storage. The method can be practiced with prior or subsequent static or perfusion hypothermic exposure of the organ. During the period in which the organ is preserved and/or maintained, the organ may be additionally be perfused to obtain data regarding the fluid and/or organ. The data may then be used to ultimately provide information regarding the perfusion efficacy and allow for altering of the perfusion parameters.

Abstract: An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. The apparatus and methods include the organ cassette with one or more openings configured to allow tubing to pass through the openings and be connected to the organ or tissue within the cassette, and including a pressure control device to allow pressure inside the portable housing to be varied.

Abstract: A cannula connects an organ with a perfusion system that monitors, treats, sustains and/or restores the viability of the organ and/or that transports and/or stores the organ. At least part of a cannula may be made of a soft elastomer to help avoid damage to the organ. In embodiments, the cannula includes a top portion, a bottom portion, a sealing ring and two compression straps. In embodiments, the cannula includes a top portion and a flexible bottom portion that may be attached to the top portion. In embodiments, part of the cannula is inserted directly into an artery and a suture is used to tie the artery in place. Also, the cannula may include an attachment feature that can be used to connect the cannula to an organ platform or chair. Various features of the cannula may allow a visual check for and venting of air bubbles in the cannula.

Abstract: A population of progenitor cells and methods for obtaining and culturing the progenitor cells, that are useful in fields including regenerative medicine (tissue regeneration), transplantation, and cancer research.

Abstract: The invention is targeted at the process of separating gas, such as air, from a liquid path. Specifically, the invention provides a means to remove gas from a dynamic liquid path, manage the removed gas and liquid path. The invention provides a means to remove gas from a dynamic liquid path using the buoyant property of gas in a less buoyant liquid, having ingress and egress ports for liquid and gas flow, and separate points of egress for liquid and trapped gas and integral liquid channels.

Abstract: Living cellular material may be preserved by incubating the cellular material in a culture medium containing at least one sugar, particularly for at least three hours, and then subjecting the cellular material to a preservation protocol, such as freezing, vitrification, freeze-drying and desiccation.

Abstract: A method for controlling a pump for delivery of liquid to an organ over a series of fixed-length time intervals f, each interval f comprising a time t1 and a time t2 wherein t1+t2 equals the length of interval f. The method comprises allowing output pressure of the pump to decrease over time t1, increasing output pressure of the pump over time t2, comparing achieved pump output pressure to a predetermined pressure at about the end of interval f, and at least one of (i) adjusting t1 and t2 if necessary so the predetermined pressure is approximated by the output pressure at the end of the next interval f, and (ii) adjusting a rate of change of the output pressure during at least one of t1 and t2 if necessary so the predetermined pressure is approximated by the output pressure at the end of the next interval f.

Abstract: An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. The method includes perfusing the organ at hypothermic and/or normothermic temperatures, preferably after hypothermic organ flushing for organ transport and/or storage. The method can be practiced with prior or subsequent static or perfusion hypothermic exposure of the organ. Organ viability is restored by restoring high energy nucleotide (e.g., ATP) levels by perfusing the organ with a medical fluid, such as an oxygenated cross-linked hemoglobin-based bicarbonate medical fluid, at normothermic temperatures.

Abstract: An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. The apparatus and methods include the organ cassette with one or more openings configured to allow tubing to pass through the openings and be connected to the organ or tissue within the cassette, and including a pressure control device to allow pressure inside the portable housing to be varied.

Abstract: An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. The apparatus and methods include the organ cassette with one or more openings configured to allow tubing to pass through the openings and be connected to the organ or tissue within the cassette, and including a pressure control device to allow pressure inside the portable housing to be varied.

Abstract: Living cellular material may be preserved by incubating the cellular material in a culture medium containing at least one sugar, particularly for at least three hours, and then subjecting the cellular material to a preservation protocol, such as freezing, vitrification, freeze-drying and desiccation.

Abstract: Living cellular material may be preserved by incubating the cellular material in a culture medium containing at least one sugar, particularly for at least three hours, and then subjecting the cellular material to a preservation protocol, such as freezing, vitrification, freeze-drying and desiccation.

Abstract: A method for vitrification of a tissue or organ includes immersing the tissue or organ in increasing concentrations of cryoprotectant solution at a temperature greater than ?15° C. to a cryoprotectant concentration sufficient for vitrification; cooling the tissue or organ at an average rate of from 2.5–100° C. per minute to a temperature between ?80° C. and the glass transition temperature; and further cooling the tissue or organ at an average rate less than 30° C. per minute to a temperature below the glass transition temperature to vitrify the tissue or organ. After the vitrified tissue or organ has been stored, the tissue or organ may be removed from vitrification by warming the tissue or organ at an average rate of from 20–40° C. per minute to a temperature between ?80° C. and the glass transition temperature; further warming the tissue or organ at a rate greater than 80° C. per minute to a temperature above ?75° C.; and reducing the concentration of the cryoprotectant.

Abstract: The invention relates to a population of progenitor cells and methods for obtaining and culturing the progenitor cells. Methods and compositions of the present invention can be useful in fields including regenerative medicine (tissue regeneration), transplantation, and cancer research.

Abstract: The disclosure relates to methods of identifying biological processes associated with non-fetal progenitor cells, and of identifying molecules and factors that regulate or modulate these processes, and is useful in fields including regenerative medicine (tissue regeneration), transplantation, and cancer research.

Abstract: An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. The method includes perfusing the organ at hypothermic and/or normothermic temperatures, preferably after hypothermic organ flushing for organ transport and/or storage. The method can be practiced with prior or subsequent static or perfusion hypothermic exposure of the organ. Organ viability is restored by restoring high energy nucleotide (e.g., ATP) levels by perfusing the organ with a medical fluid, such as an oxygenated cross-linked hemoglobin-based bicarbonate medical fluid, at normothermic temperatures. In perfusion, organ perfusion pressure is preferably controlled in response to a sensor disposed in an end of tubing placed in the organ, by a pneumatically pressurized medical fluid reservoir, providing perfusion pressure fine tuning, overpressurization prevention and emergency flow cut-off.

Abstract: A unified solution system for organ and/or tissue preservation and/or hypothermic blood substitution can be used to prepare multiple solutions for use in various stages of organ procurement, preservation and transplantation and bloodless surgery procedures.

Abstract: An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. The method includes perfusing the organ at hypothermic and/or normothermic temperatures, preferably after hypothermic organ flushing for organ transport and/or storage. The method can be practiced with prior or subsequent static or perfusion hypothermic exposure of the organ. Organ viability is restored by restoring high energy nucleotide (e.g., ATP) levels by perfusing the organ with a medical fluid, such as an oxygenated cross-linked hemoglobin-based bicarbonate medical fluid, at normothermic temperatures. In perfusion, organ perfusion pressure is preferably controlled in response to a sensor disposed in an end of tubing placed in the organ, by a pneumatically pressurized medical fluid reservoir, providing perfusion pressure fine tuning, overpressurization prevention and emergency flow cut-off.