Abstract: A method is provided for rapidly determining during a saliva specimen collection procedure the presence of an amount of saliva, and for verifying that the sample obtained is in fact saliva. Color indication by dye markers and/or enzymatic activation of color indicators provides an indication that at least a predetermined amount of saliva has been applied to an absorbent and the enzymatic reaction indicates that saliva is contained in the sample collected.
Abstract: A method of stabilizing the glucose content of a blood sample applied to a sorbent for drying is provided. A solution of sodium fluoride or other glycolysis inhibitor is applied to a sorbent. A blood sample for glucose determination is then applied to the sorbent whereupon glycolysis is immediately inhibited. The blood sample may then be dried for shipping without loss of glucose content during the drying period.
Type:
Grant
Filed:
December 17, 1991
Date of Patent:
April 20, 1993
Assignee:
Osborn Laboratories, Inc.
Inventors:
Jangbir S. Sangha, Linda R. Pflatzgraff, Melinda L. Van Hercke
Abstract: A method of gamma glutamyltransferase (GGT) analysis is suitable for use with hemolyzed fluid or dried whole blood samples which avoids the need for protein precipitation or centrifugation of the sample. Analysis is by first mixing a fluid or dried whole blood sample with a phosphate buffered saline solution containing a non-ionic biological detergent. The mixture is then vigorously agitated for one hour at room temperature. The activity of the GGT is then determined by reacting the mixture with a GGT substrate. Color is developed by a diazo coupling reaction with the enzyme reaction product. The intensity of the color is then measured.
Type:
Grant
Filed:
June 8, 1989
Date of Patent:
March 17, 1992
Assignee:
Osborn Laboratories, Inc.
Inventors:
Jane M. Rachel, Laura M. Smith, Linda R. Pfaltzgraff