Patents Assigned to Oxford Gene Technology IP Limited
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Publication number: 20130190202Abstract: A device for individually analysing cells of interest, comprising (a) a channel for receiving the contents of a cell of interest, wherein the channel has an input end and an output end, and (b) a cell trapping site in proximity to the input end of the channel, wherein (i) the input end of the channel is adapted such that an intact cell of interest cannot enter the channel; and (ii) the channel contains one or more analytical components for analysing the contents of the cell of interest. In use, a cell is applied to the device, where it is trapped by the cell trapping means. The cell cannot enter the channel intact, but its contents can be released in situ to enter the channel's input end. The contents can then move down the channel, towards the output end, and they encounter the immobilised reagents, thereby permitting analysis of the cell contents.Type: ApplicationFiled: January 17, 2013Publication date: July 25, 2013Applicant: OXFORD GENE TECHNOLOGY IP LIMITEDInventor: OXFORD GENE TECHNOLOGY IP LIMITED
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Patent number: 8372629Abstract: A device for individually analysing cells of interest, comprising (a) a channel for receiving the contents of a cell of interest, wherein the channel has an input end and an output end, and (b) a cell trapping site in proximity to the input end of the channel, wherein (i) the input end of the channel is adapted such that an intact cell of interest cannot enter the channel; and (ii) the channel contains one or more analytical components for analysing the contents of the cell of interest. In use, a cell is applied to the device, where it is trapped by the cell trapping means. The cell cannot enter the channel intact, but its contents can be released in situ to enter the channel's input end. The contents can then move down the channel, towards the output end, and they encounter the immobilised reagents, thereby permitting analysis of the cell contents.Type: GrantFiled: May 3, 2006Date of Patent: February 12, 2013Assignee: Oxford Gene Technology IP LimitedInventors: Edwin Southern, Wouter Meuleman, Dietrich Wilhelm Karl Lueerssen, Natalie Milner
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Publication number: 20120156675Abstract: A convenient way of isolating individual cells permits individual analysis of their contents. A capture support for individually capturing cells of interest comprises a first surface including at least one well sized to accommodate an individual cell, wherein the support is made of a differentially permeable material which permits transfer of a solvent and any low molecular weight species through the support from a second surface of the support to a well, but which is substantially impermeable to biopolymers. Single cells are captured, their contents are released, and the contents of individual cells are then analysed within a chamber containing suitable analytical components e.g. immobilised nucleic acid probes, immobilised antibodies, etc. Analysis of a single cell's genome, transcriptome, proteome, etc. thus becomes possible.Type: ApplicationFiled: June 9, 2010Publication date: June 21, 2012Applicant: OXFORD GENE TECHNOLOGY IP LIMITEDInventors: Dietrich Wilhelm Karl Lueerssen, Daniele Malleo
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Patent number: 8084211Abstract: A method of analysing a polynucleotide target involves incubating the target with an oligonucleotide probe, generally an array of immobilised oligonucleotide probes, to form a duplex, and using ligase or polymerase to extend one chain of the duplex. A point mutation or variable number tandem repeat section may be analysed. Arrays of immobilised oligonucleotides are provided for use in the method.Type: GrantFiled: September 7, 2006Date of Patent: December 27, 2011Assignee: Oxford Gene Technology IP LimitedInventors: Edwin Mellor Southern, Clare Elizabeth Pritchard, Stephen Charles Case-Green
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Patent number: 7399846Abstract: A method of making a set of labelled compounds by the use of a preferably particulate support, comprises dividing the support into lots, performing a different chemical reaction on each lot of the support, e.g. to couple a chemical moiety to that lot of the support, tagging a fraction of each lot of the support with a different label, and combining the said lots of the support. The steps are repeated several times, preferably to build up oligomer molecules carrying labels which identify the nature and position of a monomer unit of the oligomer, and which are releasable from the support. Preferred labels, which are releasable from the compounds by cleavage to provide charged groups for analysis by mass spectrometry, are groups of the trityl (trimethylphenyl) family. Also claimed are libraries of these labels and their use in assays and nucleic acid analysis methods.Type: GrantFiled: May 11, 2004Date of Patent: July 15, 2008Assignee: Oxford Gene Technology IP LimitedInventors: Edwin Mellor Southern, Mikhail Sergeevich Shchepinov, John Nicholas Housby, Alan Lewis Hamilton, John Kenneth Elder
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Publication number: 20080138909Abstract: Peptides can be derivatised such that, when ionised and analysed by mass spectrometry, those containing arginine residues give characteristic peak patterns. Peaks corresponding to arginine-containing peptides can therefore be selected from a mass spectrum in order to simplify and improve peptide analysis. Suitable labels give derivatised peptides that have the ability to form both a stabilised ion species ([P]+) and a protonated ion molecular species ([P+H]+) that differ by one average mass unit. A characteristic peak pattern is seen.Type: ApplicationFiled: December 8, 2004Publication date: June 12, 2008Applicant: OXFORD GENE TECHNOLOGY IP LIMITEDInventors: Susan Wheeler, Mikhail Sergeevich Shchepinov, Edwin Mellor Southern
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Patent number: 7192707Abstract: A method of analyzing a polynucleotide target involves incubating the target with an oligonucleotide probe, generally an array of immobilized oligonucleotide probes, to form a duplex, and using ligase or polymerase to extend one chain of the duplex. A point mutation or variable number tandem repeat section may be analyzed. Arrays of immobilized oligonucleotides are provided for use in the method.Type: GrantFiled: November 6, 2003Date of Patent: March 20, 2007Assignee: Oxford Gene Technology IP LimitedInventors: Edwin Mellor Southern, Clare Elizabeth Pritchard, Stephen Charles Case-Green
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Patent number: 6780981Abstract: A method of making a set of labelled compounds by the use of a preferably particulate support, comprises dividing the support into lots, performing a different chemical reaction on each lot of the support, e.g. to couple a chemical moiety to that lot of the support, tagging a fraction of each lot of the support with a different label, and combining the said lots of the support. The steps are repeated several times, preferably to build up oligomer molecules carrying labels which identify the nature and position of a monomer unit of the oligomer, and which are releasable from the support. Preferred labels, which are releasable from the compounds by cleavage to provide charged groups for analysis by mass spectrometry, are groups of the trityl (trimethylphenyl) family. Also claimed are libraries of these labels and their use in assays and nucleic acid analysis methods.Type: GrantFiled: May 8, 2001Date of Patent: August 24, 2004Assignee: Oxford Gene Technology IP LimitedInventors: Edwin Mellor Southern, Mikhail Sergeevich Shchepinov, John Nicholas Housby, Alan Lewis Hamilton, John Kenneth Elder
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Patent number: 6770751Abstract: A method of analysing a polynucleotide target involves incubating the target with an oligonucleotide probe, generally an array of immobilized oligonucleotide probes, to form a duplex, and using ligase or polymerase to extend one chain of the duplex. A point mutation or variable number tandem repeat section may be analyzed. Arrays of immobilized oligonucleotides are provided for use in the method.Type: GrantFiled: August 23, 2001Date of Patent: August 3, 2004Assignee: Oxford Gene Technology IP LimitedInventors: Edwin Mellor Southern, Clare Elizabeth Pritchard, Stephen Charles Case-Green