Patents Assigned to Oxford Nanopore Technologies Limited
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Publication number: 20200266568Abstract: A kit comprising first and second component parts adapted for connection to each other, wherein: the first component part comprises: a first array of electrical connectors; two substantially parallel lateral walls, one provided on either side of the first array of electrical connectors at a predetermined position with respect to the first array of electrical connectors; two rails provided between the first array of electrical connectors and the lateral walls, one rail being on either side of the first array of electrical connectors, wherein each rails extends at least along a length of the first array of electrical connectors, and has a front tip positioned at a predetermined position with respect to the first array of electrical connectors; a front contact point; an overhang for receiving the second component; the second component part comprises: a second array of electrical connectors, for connection to the first array of electrical connectors; a front end being configured to fit to a width between the paraType: ApplicationFiled: September 28, 2017Publication date: August 20, 2020Applicant: Oxford Nanopore Technologies LimitedInventors: David Waterman, Richard Smith
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Patent number: 10739341Abstract: The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses aptamers and transmembrane pores.Type: GrantFiled: February 14, 2013Date of Patent: August 11, 2020Assignee: Oxford Nanopore Technologies LimitedInventors: Daniel John Turner, Daniel George Fordham, Roger Charles Gill, Clive Gavin Brown, Stuart Reid, James Anthony Clarke, James White
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Publication number: 20200179920Abstract: Apparatus and methods for controlling the insertion of a membrane channel into a membrane are disclosed. In one arrangement a first bath holds a first liquid in contact with a first surface of a membrane. A second bath holds a second liquid in contact with a second surface of the membrane. The membrane separates the first and second liquids. A first electrode contacts the first liquid. A second electrode contacts the second liquid. A driving unit applies a potential difference across the membrane via the first and second electrodes to promote insertion of a membrane channel into the membrane from the first liquid or the second liquid. A membrane voltage reduction unit is connected in series with the membrane. The driving unit applies a driving voltage across the membrane voltage reduction unit and the membrane, the driving voltage providing the potential difference across the membrane.Type: ApplicationFiled: November 24, 2017Publication date: June 11, 2020Applicant: Oxford Nanopore Technologies LimitedInventors: Daniel Garalde, James Clarke, Mike Jennison, Andrew Heron
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Patent number: 10675412Abstract: A piston head for a syringe pump comprises a barrier portion (11) for driving fluid through a syringe pump barrel (30), wherein a peripheral section (13,14) of the barrier portion (11) is shaped to seal against the syringe pump barrel (30); and a re-silent member (15) arranged to resist deformation of the shaped peripheral section (13,14) of the barrier portion (11).Type: GrantFiled: September 11, 2012Date of Patent: June 9, 2020Assignee: Oxford Nanopore Technologies LimitedInventors: Anthony Jones, Jonathan Upsdell
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Publication number: 20200024654Abstract: The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore; (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.Type: ApplicationFiled: September 29, 2017Publication date: January 23, 2020Applicant: Oxford Nanopore Technologies LimitedInventors: Andrew John Heron, James Edward Graham, Richard Alexander Gutierrez, Rebecca Victoria Bowen, James White, Clive Gavin Brown, Daniel George Fordham
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Publication number: 20200010511Abstract: Provided herein relate to modified or mutant forms of secretin and compositions comprising the same. In particular, the modified or mutant forms of secretin permits efficient capture and/or translocation of an analyte through the modified or mutant secretin nanopores. Methods for using unmodified secretin or the modified or mutant forms of secretin and compositions, for example, for characterizing an analyte, e.g., a target polynucleotide, are also provided.Type: ApplicationFiled: February 12, 2018Publication date: January 9, 2020Applicant: Oxford Nanopore Technologies LimitedInventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh
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Publication number: 20190352687Abstract: The invention relates to new methods for synthesising polynucleotide molecules according to a predefined nucleotide sequence. The invention also relates to methods for the assembly of synthetic polynucleotides following synthesis, as well as systems and kits for performing the synthesis and/or assembly methods.Type: ApplicationFiled: January 19, 2018Publication date: November 21, 2019Applicant: Oxford Nanopore Technologies LimitedInventors: John Milton, Sobia Nayyar, Jan Riedl, Ryosuke Ogaki
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Publication number: 20190352709Abstract: Methods of characterizing an analyte using a nanopore. One aspect features methods for characterizing a double-stranded polynucleotide using a nanopore, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.Type: ApplicationFiled: November 29, 2017Publication date: November 21, 2019Applicant: Oxford Nanopore Technologies LimitedInventors: James Clarke, James White, Richard Muscat, Jessica Mary May Knott, Ramiz Iqbal Nathani, Andrew John Heron, Mark John Bruce, Lakmal Nishantha Jayasinghe, Domenico Caprotti, David Jackson Stoddart, Rebecca Victoria Bowen, Christopher James Wright, Paul Richard Moody
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Publication number: 20190330282Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.Type: ApplicationFiled: March 2, 2017Publication date: October 31, 2019Applicant: Oxford Nanopore Technologies LimitedInventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace
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Publication number: 20190249231Abstract: A method for determining the presence, absence or amount of two or more target polynucleotides in a sample comprising additional components, the method comprising: (i) contacting the sample with a panel of two or more probes under conditions suitable for hybridisation of the target polynucleotides to the probes, wherein: (a) each probe comprises a non-hybridisation region and a hybridisation region that specifically hybridises to one of the target polynucleotides to form a hybridised probe; and (b) the hybridisation region of a probe of the panel comprises one or more non-natural nucleotides; (ii) contacting the sample prepared in step (i) with a transmembrane pore through which a single stranded polynucleotide but not a double stranded polynucleotide can pass and applying a potential difference to the transmembrane pore such that the hybridised probes in the sample interact with the pore; (iii) measuring current blockades having a duration within a defined window, wherein: (a) the one or more non-natural nucType: ApplicationFiled: October 20, 2017Publication date: August 15, 2019Applicant: Oxford Nanopore Technologies LimitedInventors: Nicholas Antony Smith, Daniel John Turner, Daniel George Fordham, James White
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Publication number: 20190203286Abstract: A target polynucleotide is expanded. In respect of each nucleotide in the target polynucleotide, the target polynucleotide comprises clock nucleotides and at least one signal nucleotide in a predetermined order. The clock nucleotides have a predetermined sequence common to each nucleotide in the target polynucleotide. The at least one signal nucleotide is characteristic of the identity of the respective nucleotide in the target polynucleotide. During translocation of the expanded polynucleotide through a nanopore, electrical measurements dependent on the polynucleotide within the pore are made, to derive an analysis signal. Clock signals derived from the clock nucleotides are identified. Relative to the positions of the identified clock signals, nucleotide signals derived from the least one signal nucleotide are derived to analyse the target polynucleotide.Type: ApplicationFiled: October 17, 2018Publication date: July 4, 2019Applicant: Oxford Nanopore Technologies LimitedInventors: Stuart William Reid, Gavin Harper
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Publication number: 20190202876Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using the mutant forms of lysenin.Type: ApplicationFiled: April 6, 2017Publication date: July 4, 2019Applicant: Oxford Nanopore Technologies LimitedInventors: Lakmal Jayasinghe, Mark John Bruce, Luke McNeill, Ramiz Iqbal Nathani, Pratik Raj Singh, Neil Roger Wood, Stephen Robert Young
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Publication number: 20190071721Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.Type: ApplicationFiled: March 2, 2017Publication date: March 7, 2019Applicant: Oxford Nanopore Technologies LimitedInventors: Lakmal JAYASINGHE, Elizabeth Jayne WALLACE
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Publication number: 20190032798Abstract: A one-way valve, comprises a valve housing; a valve member provided within the valve housing, the valve member being operative to open and close the valve and comprising a diaphragm with a central orifice to allow fluid to pass from one side of the diaphragm to the other; a valve inlet provided on a first side of the diaphragm; and a valve outlet provided on a second side of the diaphragm; wherein the configuration of the diaphragm and the valve housing biases the diaphragm to seal the inlet at rest.Type: ApplicationFiled: June 26, 2018Publication date: January 31, 2019Applicant: Oxford Nanopore Technologies LimitedInventors: Anthony Jones, Jonathan Upsdell
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Patent number: 10054234Abstract: A one-way valve, comprises a valve housing; a valve member provided within the valve housing, the valve member being operative to open and close the valve and comprising a diaphragm with a central orifice to allow fluid to pass from one side of the diaphragm to the other; a valve inlet provided on a first side of the diaphragm; and a valve outlet provided on a second side of the diaphragm; wherein the configuration of the diaphragm and the valve housing biases the diaphragm to seal the inlet at rest.Type: GrantFiled: July 11, 2012Date of Patent: August 21, 2018Assignee: Oxford Nanopore Technologies LimitedInventors: Anthony Jones, Jonathan Upsdell
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Patent number: 10036065Abstract: An analysis apparatus for performing biochemical analysis of a sample using nanopores comprises: a sensor device that that supports plural nanopores, reservoirs holding material for performing the analysis; a fluidics system; and plural containers for receiving respective samples, all arranged in a cartridge that is removably attachable to an electronics unit arranged to generate drive signals to perform signal processing circuit to generate output data representing the results of the analysis. The fluidics system supplies samples selectively from the containers to the sensor device using a rotary valve. In one valve, a stator defines a plurality of first ports arranged around the rotational axis and a collection chamber extending in around the axis of rotation in communication with a second port. A rotor provides a passage extending between the collection chamber and a position in communication with any one of the plurality of first ports.Type: GrantFiled: January 30, 2017Date of Patent: July 31, 2018Assignee: Oxford Nanopore Technologies LimitedInventor: Anthony Jones
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Patent number: 9885078Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.Type: GrantFiled: August 8, 2014Date of Patent: February 6, 2018Assignee: Oxford Nanopore Technologies LimitedInventors: Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Anthony Clarke
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Patent number: 9797009Abstract: The invention relates to methods using constructs comprising a helicase and an additional polynucleotide binding moiety. The helicase is attached to the polynucleotide binding moiety and the construct has the ability to control the movement of a polynucleotide. The constructs can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.Type: GrantFiled: July 18, 2013Date of Patent: October 24, 2017Assignee: Oxford Nanopore Technologies LimitedInventors: Andrew Heron, James Clarke, Ruth Moysey, Elizabeth Jayne Wallace, Mark Bruce, Lakmal Jayasinghe, Domenico Caprotti, Szabolcs Soeroes, Luke McNeill, Mihaela Misca
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Patent number: 9758823Abstract: The invention relates to a new method of characterizing a target polynucleotide. The method uses a pore and a Hel308 helicase or amolecular motor which is capable of binding to the target polynucleotide at an internal nucleotide. The helicase or molecular motor controls the movement of the target polynucleotide through the pore.Type: GrantFiled: October 18, 2012Date of Patent: September 12, 2017Assignee: Oxford Nanopore Technologies LimitedInventors: Ruth Moysey, Andrew John Heron
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Patent number: 9651519Abstract: An analysis instrument comprises plural modules connected together over a data network, each module comprising an analysis apparatus operable to perform biochemical analysis of a sample. Each module comprises a control unit that controls the operation of the analysis apparatus. The control units are addressable to select an arbitrary number of modules to operate as a cluster for performing a common biochemical analysis. The control units communicate over the data network, repeatedly during the performance of the common biochemical analysis, to determine the operation of the analysis apparatus of each module required to meet the global performance targets, on the basis of measures of performance derived from the output data produced by the modules. The arrangement of the instrument as modules interacting in this manner provides a scalable analysis instrument.Type: GrantFiled: July 29, 2015Date of Patent: May 16, 2017Assignee: Oxford Nanopore Technologies LimitedInventors: Clive Gavin Brown, James Peter Willcocks