Abstract: A method of real-time imaging of dissolved oxygen concentration, comprising adding an oxygen-quenched phosphorescent composition to a sample, exciting phosphorescence in the composition by illuminating the sample with pulses of light, detecting phosphorescence intensity as a function of position in the sample at first and second times following exciting pulses of light, determining oxygen concentration from the phosphorescence detected at the first and second times, generating an image of the oxygen concentration as a function of position, and repeating the exciting, detecting, determining, and image generating steps to produce a series of images showing the oxygen concentration varying over time.
Abstract: Provided is a method and system for the rapid and accurate detection of growth and metabolism of a cellular microorganism in a population of microorganisms in a non-liquid, culture medium. Further provided is a gelled culture medium containing a non-toxic, water-soluble, phosphorescent compound which measures oxygen content (partial pressure) of an microorganism also contained therein, by oxygen-dependent quenching of phosphorescence; or the gel contains a fluorescent pH indicator that demonstrates growth of the microorganism by pH-dependent intensity change or wavelength shift in the emission spectrum. Further provided is a system and method for killing undesirable microorganisms or colonies in the culture medium without harming the surrounding microorganisms.
Abstract: An illuminator for use in a darkroom or with scientific instrumentation with several light emitting diodes arranged in a regular array, a transparent structure to house the LED array, two circular end caps to prevent radiation from the end zones, an opaque decorative adhesive film surrounding the outer surface of the cylinder housing, an attachment means comprising hook and loop strips with adhesive backing and a two wire lead originating in a connector to bring power to the LED array.
Type:
Grant
Filed:
August 13, 1998
Date of Patent:
May 2, 2000
Assignee:
Oxygen Enterprises, Ltd.
Inventors:
David F. Wilson, Benjamin W. Dugan, Jennifer A. Johnson