Abstract: An isolated chimeric recombinant protein has cutinase activity. The protein includes a cutinase catalytic domain and a polymer binding domain operably linked by a proline/threonine-rich linker domain. The proline/threonine-rich linker domain includes at least 50% proline or threonine residues over a stretch of 15 to 55 consecutive amino acids.
Type:
Grant
Filed:
August 9, 2017
Date of Patent:
March 12, 2019
Assignees:
Ozymes, National Research Council of Canada
Inventors:
Kofi Abokitse, Peter C. K. Lau, Stephan Grosse
Abstract: Multi-domain recombinant proteins have a cutinase catalytic domain. The cutinase catalytic domain is a modular domain, which may be combined with other protein domains that also function in a modular fashion. For example, the cutinase catalytic domain can be operably linked to a polymer binding domain via a linker domain (e.g., a threonine/proline-rich linker polypeptide). The cutinase catalytic domain can be from an endogenous cutinase having a multi-domain modular organization, such as from the actinobacterium Kineococcus radiotolerans. The recombinant proteins are used in compositions, methods and uses, such as for use in industrial applications.
Type:
Grant
Filed:
February 16, 2016
Date of Patent:
September 5, 2017
Assignees:
NATIONAL RESEARCH COUNCIL OF CANADA, OZYMES
Inventors:
Kofi Abokitse, Peter C. K. Lau, Stephan Grosse