Abstract: Disclosed herein are methods and compositions (e.g., oligonucleotide primers) for isothermal amplification and detection of M. pneumoniae nucleic acids in a sample. In some embodiments, the methods include contacting a sample with a set of LAMP primers specific for a M. pneumoniae CARDS toxin-encoding nucleic acid under conditions sufficient to produce an M. pneumoniae nucleic acid amplification product and detecting the resulting M. pneumoniae amplification product. Kits including sets of LAMP primers for detection of M. pneumoniae CARDS toxin nucleic acids are also provided herein.
Type:
Grant
Filed:
February 12, 2016
Date of Patent:
March 19, 2019
Assignees:
The United States of America, as represented by the Secretary, Department of Health and Human Services, P3S Corporation
Inventors:
Jonas M. Winchell, Brianna Petrone, Maureen H. Diaz, Bernard J. Wolff