Abstract: An ultrasonic air-in-line detection system for use in detecting air bubbles in the fluid line of a disposable cassette mounted on a main pump unit is disclosed in which a self-test procedure is periodically used to ensure that any faults in the ultrasonic air-in-line detector which do not fail safe are automatically detected. After a pumping cycle is completed, if the ultrasonic air-in-line detector indicates that there is fluid in the fluid line at the location of the ultrasonic sensor, the operating frequency of the transmitting ultrasonic transducer is changed to a non-resonant frequency for the self-test procedure. If the ultrasonic air-in-line detector still produces a signal indicating that there is fluid in the line, this indicates that there is a failure in the ultrasonic detector and a fault is indicated and the system is shut down.