Abstract: The invention relates to compositions and methods for targeting sequence modifications in one or more genes of a related family of genes using enhanced homologous recombination techniques. These techniques may be used to create animal or plant models of disease as well as to identify new targets for drug or pathogen screening.

Abstract: Compositions and methods are provided for rapidly evolving specific protein domains employing a recombinase and recombination intermediates.

Abstract: Methods and compositions are providing for modulating cellular activity. In the subject methods, target cells are contacted with both a BER inhibitor and a sensitizing agent, e.g., either a radiosensitizing agent and/or a chemotherapeutic agent, where the cells may optionally be contacted with a DSBR inhibitor, such as a RAD inhibitor, e.g., a RAD51 inhibitor. Also provided are pharmaceutical preparations, as well as kits thereof, that find use in practicing the subject methods.

Abstract: Provided herein are methods for inhibiting cell proliferation in an individual comprising administering to the individual a composition comprising a Rad51 inhibitor. Also provided herein is a method for inhibiting the growth of a cell comprising administering to said cell a composition comprising a Rad51 inhibitor. Such methods can further include the step of providing radiation or DNA damaging agents after administration of said Rad51 inhibitor. Also described herein are methods which are performed in vivo and/or on cancerous cells.

Abstract: The invention relates to compositions and methods for targeting sequence modifications in one or more genes of a related family of genes using enhanced homologous recombination techniques. These techniques may be used to create animal or plant models of disease as well as to identify new targets for drug or pathogen screening.

Abstract: Nucleoproteins comprising overlapping homologous DNA sequences coated with a recombinase are employed for introduction into cells to produce double D-loop structures. The sequences may be modified with various moieties which result in modification of DNA. Formation of the D-loops results in inhibition of replication and transcription, where the moieties can be employed for permanent scission or modification of the DNA. The methods and compositions can be used for investigating physiological processes, for producing animal models, and for inhibition of an undesirable phenotype in vivo.