Patents Assigned to Panomics, Inc.
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Publication number: 20120046200Abstract: A method is provided for screening for agents that affect protein degradation rates, the method comprising: taking a library of cells, the cells expressing a fusion protein comprising a reporter protein and a protein encoded by a sequence from a cDNA library derived from a sample of cells, the sequence from the cDNA library varying within the cell library; contacting the library of cells with a plurality of agents which may affect protein degradation rates; for each agent, selecting cells in the library which express short-lived proteins based on whether the cells have different reporter signal intensities than other cells in the library, the difference being indicative of the selected cells expressing shorter lived fusion proteins than the fusion proteins expressed by the other cells in the library; and characterizing the fusion proteins expressed by the selected cells for each agent.Type: ApplicationFiled: November 3, 2011Publication date: February 23, 2012Applicant: Panomics, Inc.Inventors: Xianqiang Li, Xin Jiang
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Publication number: 20110256536Abstract: This invention provides methods, compositions and systems to detect a nucleic acid of interest in a two-stage amplification. The two-stage amplification begins with a first non-enzymatic accumulation of an amplification oligomer that is the target substrate for a second nucleic acid amplification or assay. Two or more amplification oligomers can be used to allow multiplexed amplifications of two or more nucleic acids of interest with deconvolution based on unique detection signals or unique signal locations.Type: ApplicationFiled: April 21, 2011Publication date: October 20, 2011Applicant: Panomics, Inc.Inventors: Gary McMaster, Yunqing Ma
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Publication number: 20110223606Abstract: This invention provides methods of quantitating nucleic acids from problematic samples, such as aged samples, formalin fixed samples, paraffin embedded samples, samples with aneuploid cells, and cells with fragmented nucleic acids. Methods include techniques to efficiently solubilize the nucleic acids under non-denaturing conditions from preserved clinical samples without resort to organic extractions, to normalize cell counts regardless of aneuploidy, to access the fragmentation state of the nucleic acids, and to provide standard curves for degraded nucleic acid samples.Type: ApplicationFiled: May 17, 2011Publication date: September 15, 2011Applicant: Panomics, Inc.Inventors: Gary McMaster, Joan Davies, Yunqing Ma, Yuling Luo
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Patent number: 8017360Abstract: Methods for detecting and optionally quantitating one or more target nucleic acids are provided, in which a surrogate nucleic acid is captured to each target nucleic acid, amplified, and detected. Compositions, kit, and systems related to the methods are also described.Type: GrantFiled: November 9, 2006Date of Patent: September 13, 2011Assignee: Panomics, Inc.Inventors: Yuling Luo, Son Bui
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Patent number: 7981842Abstract: A method is provided for identifying complexes between a transcription factor and another protein, such as another different transcription factor. The method includes the steps of isolating from a biological sample transcription factor complexes based on whether the transcription factor complexes include a particular type of transcription factor; and identifying which of the multiple different proteins are present in the isolated transcription factor complexes.Type: GrantFiled: September 5, 2001Date of Patent: July 19, 2011Assignee: Panomics, Inc.Inventor: Xianqiang Li
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Patent number: 7951539Abstract: This invention provides methods, compositions and systems to detect a nucleic acid of interest in a two-stage amplification. The two-stage amplification begins with a first non-enzymatic accumulation of an amplification oligomer that is the target substrate for a second nucleic acid amplification or assay. Two or more amplification oligomers can be used to allow multiplexed amplifications of two or more nucleic acids of interest with deconvolution based on unique detection signals or unique signal locations.Type: GrantFiled: October 7, 2009Date of Patent: May 31, 2011Assignee: Panomics, Inc.Inventors: Gary McMaster, Yunqing Ma
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Patent number: 7927798Abstract: Methods of detecting one or more nucleic acids from whole blood or plasma are provided. The nucleic acids are captured on a solid support and detected. Compositions, kits, and systems related to the methods are also described.Type: GrantFiled: October 4, 2006Date of Patent: April 19, 2011Assignee: Panomics, Inc.Inventors: Zhi Zheng, Yuling Luo, Gary McMaster
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Patent number: 7803541Abstract: Methods of detecting two or more nucleic acids in a multiplex branched-chain DNA assay are provided. Different nucleic acids are captured through cooperative hybridization events on different, identifiable subsets of particles or at different selected positions on a spatially addressable solid support. Compositions, kits, and systems related to the methods are also described.Type: GrantFiled: May 11, 2006Date of Patent: September 28, 2010Assignee: Panomics, Inc.Inventors: Yuling Luo, Wen Yang
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Patent number: 7790378Abstract: A method is provided for screening for agents that affect protein degradation rates, the method comprising: taking a library of cells, the cells expressing a fusion protein comprising a reporter protein and a protein encoded by a sequence from a cDNA library derived from a sample of cells, the sequence from the cDNA library varying within the cell library; contacting the library of cells with a plurality of agents which may affect protein degradation rates; for each agent, selecting cells in the library which express short-lived proteins based on whether the cells have different reporter signal intensities than other cells in the library, the difference being indicative of the selected cells expressing shorter lived fusion proteins than the fusion proteins expressed by the other cells in the library; and characterizing the fusion proteins expressed by the selected cells for each agent.Type: GrantFiled: December 14, 2005Date of Patent: September 7, 2010Assignee: Panomics, Inc.Inventors: Xianqiang Li, Xin Jiang
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Publication number: 20100099626Abstract: The present invention concerns cell-penetrating peptides which comprise an amino acid sequence consisting of GLX9WRAX9WRX1LX2RSLX9WX3X4X5X6X7X8 (SEQ ID No: 1), wherein X1 is A, L or G, X2 is W or none, X3 is R or K, X4 is K, L or S, X5 is L or K, X6 is R or W, X7 is K or S, and X8 is A, V or Q, and X9 is W, F or Y. These CPPs can be used as vectors for delivering nucleic acids and/or proteins and/or peptides to cells, in vitro or in vivo.Type: ApplicationFiled: December 30, 2008Publication date: April 22, 2010Applicants: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, PANOMICS, INC.Inventors: Gilles DIVITA, Frederic HEITZ, Catherine MORRIS, Gudrun ALDRIAN-HERRADA
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Publication number: 20090292003Abstract: The present invention concerns cell-penetrating peptides which comprise an amino acid sequence consisting of GLX9RALX9RX1LX2RSLX9X3X4X5X6X7X8 (SEQ ID No: 1), wherein X1 is A, L or G, X2 is W or none, X3 is R or K, X4 is K, L or S, X5 is L or K, X6 is R or W, X7 is K or S, and X8 is A, V or Q, and X9 is W, F or Y. These CPPs can be used as vectors for delivering nucleic acids and/or proteins and/or peptides to cells, in vitro or in vivo.Type: ApplicationFiled: December 5, 2006Publication date: November 26, 2009Applicants: Centre National De La Recherche Scientifique, Panomics Inc.Inventors: Gilles Divita, Frederic Heitz, May Catherine Morris, Gudrun Aldrian-Herrada
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Patent number: 7615351Abstract: This invention provides methods, compositions and systems to detect a nucleic acid of interest in a two-stage amplification. The two-stage amplification begins with a first non-enzymatic accumulation of an amplification oligomer that is the target substrate for a second nucleic acid amplification or assay. Two or more amplification oligomers can be used to allow multiplexed amplifications of two or more nucleic acids of interest with deconvolution based on unique detection signals or unique signal locations.Type: GrantFiled: November 6, 2007Date of Patent: November 10, 2009Assignee: Panomics, Inc.Inventors: Gary McMaster, Yunqing Ma
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Patent number: 7579318Abstract: The present invention concerns cell-penetrating peptides which comprise an amino acid sequence consisting of GLX9WRAX9WRX1LX2RSLX9WX3X4X5X6X7X8 (SEQ ID No: 1), wherein X1 is A, L or G, X2 is W or none, X3 is R or K, X4 is K, L or S, X5 is L or K, X6 is R or W, X7 is K or S, and X8 is A, V or Q, and X9 is W, F or Y. These CPPs can be used as vectors for delivering nucleic acids and/or proteins and/or peptides to cells, in vitro or in vivo.Type: GrantFiled: December 6, 2005Date of Patent: August 25, 2009Assignees: Centre de la Recherche de la Scientifique, Panomics, Inc.Inventors: Gilles Divita, Frédéric Heitz, Catherine May Morris, Gudrun Aldrian-Herrada
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Patent number: 7551271Abstract: Uncaging devices that can be used to uncage photoactivatable caged components are provided. Consistent, uniform and/or high throughput processing of reactions and assays that include caged components is provided. Masked multiwell plates that can be used for uncaging photoactivatable caged components are provided. Methods and apparatus for initiating assays involving caged components are provided.Type: GrantFiled: August 13, 2007Date of Patent: June 23, 2009Assignee: Panomics, Inc.Inventors: Frank Witney, Gary McMaster, Quan Nguyen, Steve Chen
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Patent number: 7541193Abstract: A number of caged (e.g., photoactivatable) compositions are provided, for example, caged enzyme sensors, caged binding sensors, caged nucleic acid probes, caged interfering RNAs, caged antisense nucleic acids, caged ribozymes, caged biomolecular analogs, caged transcription factors, caged antibodies, caged molecular decoys, and caged aptamers. Compositions comprising a plurality of caged components are also provided, as are labeled and optionally caged modulators. Also provided are kits for making the caged compositions and methods for making and for using the caged compositions. Related systems and devices are also provided (e.g., an uncaging device that can trigger photoactivatable assays and optionally detect signals from the assays).Type: GrantFiled: November 17, 2003Date of Patent: June 2, 2009Assignee: Panomics, Inc.Inventors: Quan Nguyen, Gary McMaster
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Publication number: 20090131269Abstract: Methods are provided for detecting and optionally quantitating multiple analytes, including nucleic acid and/or polypeptide analytes, in particle-based assays that can be highly multiplexed. Compositions, systems, and kits related to the methods are also featured.Type: ApplicationFiled: October 2, 2008Publication date: May 21, 2009Applicant: Panomics, Inc.Inventors: Jason Martin, Quan Ngoc Nguyen
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Publication number: 20080176242Abstract: This invention provides methods, compositions and systems to detect a nucleic acid of interest in a two-stage amplification. The two-stage amplification begins with a first non-enzymatic accumulation of an amplification oligomer that is the target substrate for a second nucleic acid amplification or assay. Two or more amplification oligomers can be used to allow multiplexed amplifications of two or more nucleic acids of interest with deconvolution based on unique detection signals or unique signal locations.Type: ApplicationFiled: November 6, 2007Publication date: July 24, 2008Applicant: Panomics, Inc.Inventors: Gary McMaster, Yunqing Ma
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Publication number: 20080050746Abstract: This invention provides methods of quantitating nucleic acids from problematic samples, such as aged samples, formalin fixed samples, paraffin embedded samples, samples with aneuploid cells, and cells with fragmented nucleic acids. Methods include techniques to efficiently solublize the nucleic acids under non-denaturing conditions from preserved clinical samples without resort to organic extractions, to normalize cell counts regardless of aneuploidy, to access the fragmentation state of the nucleic acids, and to provide standard curves for degraded nucleic acid samples.Type: ApplicationFiled: August 14, 2007Publication date: February 28, 2008Applicant: Panomics, Inc.Inventors: Gary McMaster, Joan Davies, Yunqing Ma, Yuling Luo
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Publication number: 20080030713Abstract: Uncaging devices that can be used to uncage photoactivatable caged components are provided. Consistent, uniform and/or high throughput processing of reactions and assays that include caged components is provided. Masked multiwell plates that can be used for uncaging photoactivatable caged components are provided. Methods and apparatus for initiating assays involving caged components are provided.Type: ApplicationFiled: August 13, 2007Publication date: February 7, 2008Applicant: Panomics, Inc.Inventors: Frank Witney, Gary McMaster, Quan Nguyen, Steve Chen
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Patent number: 7271886Abstract: Uncaging devices that can be used to uncage photoactivatable caged components are provided. Consistent, uniform and/or high throughput processing of reactions and assays that include caged components is provided. Masked multiwell plates that can be used for uncaging photoactivatable caged components are provided. Methods and apparatus for initiating assays involving caged components are provided.Type: GrantFiled: November 17, 2003Date of Patent: September 18, 2007Assignee: Panomics, Inc.Inventors: Frank Witney, Gary McMaster, Quan Nguyen, Steve Chen