Patents Assigned to Panvera, Inc.
  • Process for reverse transcriptase activity measurement using fluorescence polarization
    Patent number: 6100039
    Abstract: Described is a process for detecting reverse transcriptase activity and, thereby, reverse transcriptase inhibitors using fluorescence polarization, comprising, mixing a DNA primer with an RNA template. Then forming an RNA/DNA duplex utilizing the reverse transcriptase and removing the RNA from the RNA/DNA duplex to form single-stranded DNA. Finally, adding a fluorescent-labeled oligonucleotide complementary to the single-stranded DNA for hybridizing to the single-stranded DNA; and, measuring the fluorescence polarization.
    Type: Grant
    Filed: January 28, 1999
    Date of Patent: August 8, 2000
    Assignee: Panvera, Inc.
    Inventors: Thomas J. Burke, Randall E. Bolger, Francis J. Lenoch